Experiment: H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01205 and MMP_RS01210 are separated by 182 nucleotides MMP_RS01210 and MMP_RS01215 are separated by 197 nucleotides
MMP_RS01205: MMP_RS01205 - manganese-dependent inorganic pyrophosphatase, at 226,294 to 227,217
_RS01205
MMP_RS01210: MMP_RS01210 - sodium:solute symporter, at 227,400 to 228,833
_RS01210
MMP_RS01215: MMP_RS01215 - sodium/proline symporter PutP, at 229,031 to 230,509
_RS01215
Position (kb) 227
228
229 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 227.039 kb on - strand at 227.151 kb on - strand at 227.184 kb on + strand at 227.279 kb on + strand at 227.281 kb on + strand at 227.308 kb on + strand at 227.310 kb on + strand at 227.326 kb on - strand at 227.326 kb on - strand at 227.363 kb on - strand at 227.363 kb on - strand at 227.364 kb on + strand at 227.373 kb on - strand at 227.373 kb on - strand at 227.374 kb on + strand at 227.407 kb on - strand at 227.408 kb on + strand at 227.588 kb on + strand, within MMP_RS01210 at 227.636 kb on + strand, within MMP_RS01210 at 227.636 kb on + strand, within MMP_RS01210 at 227.792 kb on + strand, within MMP_RS01210 at 228.056 kb on - strand, within MMP_RS01210 at 228.197 kb on - strand, within MMP_RS01210 at 228.214 kb on + strand, within MMP_RS01210 at 228.215 kb on - strand, within MMP_RS01210 at 228.215 kb on - strand, within MMP_RS01210 at 228.287 kb on - strand, within MMP_RS01210 at 228.292 kb on - strand, within MMP_RS01210 at 228.292 kb on - strand, within MMP_RS01210 at 228.419 kb on + strand, within MMP_RS01210 at 228.694 kb on + strand at 228.813 kb on - strand at 228.818 kb on + strand at 228.908 kb on + strand at 228.947 kb on + strand at 228.950 kb on - strand at 228.950 kb on - strand at 228.950 kb on - strand at 228.951 kb on + strand at 229.030 kb on - strand at 229.036 kb on + strand at 229.099 kb on - strand at 229.238 kb on - strand, within MMP_RS01215 at 229.293 kb on + strand, within MMP_RS01215 at 229.542 kb on - strand, within MMP_RS01215 at 229.555 kb on - strand, within MMP_RS01215 at 229.598 kb on + strand, within MMP_RS01215 at 229.675 kb on - strand, within MMP_RS01215 at 229.714 kb on + strand, within MMP_RS01215 at 229.731 kb on - strand, within MMP_RS01215 at 229.731 kb on - strand, within MMP_RS01215 at 229.732 kb on + strand, within MMP_RS01215 at 229.732 kb on + strand, within MMP_RS01215 at 229.732 kb on + strand, within MMP_RS01215 at 229.750 kb on - strand, within MMP_RS01215 at 229.751 kb on + strand, within MMP_RS01215 at 229.790 kb on - strand, within MMP_RS01215
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin remove 227,039 - -0.3 227,151 - +3.4 227,184 + +0.4 227,279 + -0.8 227,281 + -0.6 227,308 + +0.9 227,310 + -0.3 227,326 - +0.1 227,326 - -0.2 227,363 - -0.1 227,363 - -0.0 227,364 + -0.7 227,373 - -0.2 227,373 - +0.3 227,374 + -0.3 227,407 - +0.8 227,408 + -0.3 227,588 + MMP_RS01210 0.13 -0.7 227,636 + MMP_RS01210 0.16 -0.2 227,636 + MMP_RS01210 0.16 -0.7 227,792 + MMP_RS01210 0.27 +2.1 228,056 - MMP_RS01210 0.46 +0.4 228,197 - MMP_RS01210 0.56 -2.0 228,214 + MMP_RS01210 0.57 -0.4 228,215 - MMP_RS01210 0.57 +0.1 228,215 - MMP_RS01210 0.57 +0.3 228,287 - MMP_RS01210 0.62 -0.6 228,292 - MMP_RS01210 0.62 +0.8 228,292 - MMP_RS01210 0.62 -0.8 228,419 + MMP_RS01210 0.71 -0.8 228,694 + -0.2 228,813 - -0.3 228,818 + -0.5 228,908 + -0.6 228,947 + -1.1 228,950 - -0.0 228,950 - -0.2 228,950 - +0.6 228,951 + -0.4 229,030 - -1.9 229,036 + -1.5 229,099 - +0.3 229,238 - MMP_RS01215 0.14 -1.5 229,293 + MMP_RS01215 0.18 -0.7 229,542 - MMP_RS01215 0.35 -0.3 229,555 - MMP_RS01215 0.35 -0.0 229,598 + MMP_RS01215 0.38 -0.4 229,675 - MMP_RS01215 0.44 -0.5 229,714 + MMP_RS01215 0.46 +1.6 229,731 - MMP_RS01215 0.47 -0.5 229,731 - MMP_RS01215 0.47 -1.4 229,732 + MMP_RS01215 0.47 -0.0 229,732 + MMP_RS01215 0.47 -0.2 229,732 + MMP_RS01215 0.47 +0.1 229,750 - MMP_RS01215 0.49 -0.4 229,751 + MMP_RS01215 0.49 +0.0 229,790 - MMP_RS01215 0.51 -0.9
Or see this region's nucleotide sequence