Strain Fitness in Methanococcus maripaludis S2 around MMP_RS00740

Experiment: H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS00735 and MMP_RS00740 are separated by 306 nucleotidesMMP_RS00740 and MMP_RS00745 are separated by 77 nucleotidesMMP_RS00745 and MMP_RS00750 are separated by 15 nucleotides MMP_RS00735: MMP_RS00735 - 5 10-methenyltetrahydromethanopterin hydrogenase, at 133,625 to 134,689 _RS00735 MMP_RS00740: MMP_RS00740 - cyclase family protein, at 134,996 to 135,565 _RS00740 MMP_RS00745: MMP_RS00745 - histidinol phosphate phosphatase domain-containing protein, at 135,643 to 136,299 _RS00745 MMP_RS00750: MMP_RS00750 - fumarate hydratase, at 136,315 to 137,160 _RS00750 Position (kb) 134 135 136Strain fitness (log2 ratio) -2 -1 0 1 2at 134.255 kb on - strand, within MMP_RS00735at 134.255 kb on - strand, within MMP_RS00735at 134.424 kb on + strand, within MMP_RS00735at 134.713 kb on - strandat 134.713 kb on - strandat 134.723 kb on - strandat 134.757 kb on + strandat 134.760 kb on - strandat 134.760 kb on - strandat 134.782 kb on + strandat 134.863 kb on - strandat 134.900 kb on + strandat 134.982 kb on + strandat 135.287 kb on - strand, within MMP_RS00740at 135.338 kb on + strand, within MMP_RS00740at 135.421 kb on - strand, within MMP_RS00740at 135.422 kb on + strand, within MMP_RS00740at 135.565 kb on - strandat 135.600 kb on + strandat 135.606 kb on + strandat 135.613 kb on - strandat 135.613 kb on - strandat 135.619 kb on + strandat 135.675 kb on - strandat 135.800 kb on - strandat 136.395 kb on + strandat 136.454 kb on - strand, within MMP_RS00750

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin
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134,255 - MMP_RS00735 0.59 +0.1
134,255 - MMP_RS00735 0.59 -2.3
134,424 + MMP_RS00735 0.75 -2.3
134,713 - +0.8
134,713 - -0.5
134,723 - -1.3
134,757 + -1.0
134,760 - -0.3
134,760 - -0.2
134,782 + +1.2
134,863 - -0.1
134,900 + -0.7
134,982 + +0.0
135,287 - MMP_RS00740 0.51 -1.9
135,338 + MMP_RS00740 0.60 -0.8
135,421 - MMP_RS00740 0.75 +0.0
135,422 + MMP_RS00740 0.75 -0.5
135,565 - +0.2
135,600 + +0.8
135,606 + +0.1
135,613 - +1.1
135,613 - +2.4
135,619 + +1.1
135,675 - +0.4
135,800 - +0.2
136,395 + -0.4
136,454 - MMP_RS00750 0.16 +0.6

Or see this region's nucleotide sequence