Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17610 and BWI76_RS17615 are separated by 59 nucleotides BWI76_RS17615 and BWI76_RS17620 are separated by 179 nucleotides BWI76_RS17620 and BWI76_RS17625 overlap by 8 nucleotides BWI76_RS17625 and BWI76_RS17630 overlap by 1 nucleotides
BWI76_RS17610: BWI76_RS17610 - LysR family transcriptional regulator, at 3,658,719 to 3,659,612
_RS17610
BWI76_RS17615: BWI76_RS17615 - RNA pseudouridine synthase, at 3,659,672 to 3,660,358
_RS17615
BWI76_RS17620: BWI76_RS17620 - ABC transporter ATP-binding protein, at 3,660,538 to 3,661,626
_RS17620
BWI76_RS17625: BWI76_RS17625 - quaternary amine ABC transporter permease, at 3,661,619 to 3,662,248
_RS17625
BWI76_RS17630: BWI76_RS17630 - binding-protein-dependent transport system inner membrane protein, at 3,662,248 to 3,662,916
_RS17630
Position (kb)
3660
3661
3662 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3659.675 kb on - strand at 3659.733 kb on + strand at 3659.938 kb on + strand, within BWI76_RS17615 at 3660.052 kb on + strand, within BWI76_RS17615 at 3660.222 kb on + strand, within BWI76_RS17615 at 3660.298 kb on + strand at 3660.360 kb on - strand at 3660.387 kb on + strand at 3660.476 kb on + strand at 3660.476 kb on + strand at 3660.476 kb on + strand at 3660.477 kb on + strand at 3660.484 kb on + strand at 3660.485 kb on + strand at 3660.490 kb on + strand at 3660.490 kb on + strand at 3660.495 kb on + strand at 3660.501 kb on - strand at 3660.607 kb on - strand at 3660.628 kb on - strand at 3660.638 kb on + strand at 3660.683 kb on - strand, within BWI76_RS17620 at 3660.698 kb on - strand, within BWI76_RS17620 at 3660.728 kb on + strand, within BWI76_RS17620 at 3660.736 kb on - strand, within BWI76_RS17620 at 3661.137 kb on - strand, within BWI76_RS17620 at 3661.172 kb on - strand, within BWI76_RS17620 at 3661.335 kb on + strand, within BWI76_RS17620 at 3661.670 kb on - strand at 3661.687 kb on - strand, within BWI76_RS17625 at 3661.969 kb on + strand, within BWI76_RS17625 at 3662.070 kb on - strand, within BWI76_RS17625 at 3662.121 kb on - strand, within BWI76_RS17625 at 3662.132 kb on + strand, within BWI76_RS17625 at 3662.148 kb on + strand, within BWI76_RS17625 at 3662.171 kb on - strand, within BWI76_RS17625 at 3662.171 kb on - strand, within BWI76_RS17625 at 3662.171 kb on - strand, within BWI76_RS17625 at 3662.171 kb on - strand, within BWI76_RS17625 at 3662.175 kb on + strand, within BWI76_RS17625 at 3662.180 kb on + strand, within BWI76_RS17625 at 3662.180 kb on + strand at 3662.183 kb on - strand, within BWI76_RS17625 at 3662.188 kb on - strand at 3662.189 kb on - strand at 3662.200 kb on - strand at 3662.226 kb on + strand at 3662.226 kb on + strand at 3662.226 kb on + strand at 3662.226 kb on + strand at 3662.226 kb on + strand at 3662.226 kb on + strand at 3662.226 kb on + strand at 3662.226 kb on + strand at 3662.234 kb on - strand at 3662.234 kb on - strand at 3662.234 kb on - strand at 3662.234 kb on - strand at 3662.234 kb on - strand at 3662.234 kb on - strand at 3662.247 kb on + strand at 3662.266 kb on + strand at 3662.292 kb on + strand at 3662.295 kb on - strand at 3662.305 kb on - strand at 3662.352 kb on + strand, within BWI76_RS17630 at 3662.355 kb on - strand, within BWI76_RS17630 at 3662.355 kb on - strand, within BWI76_RS17630 at 3662.360 kb on - strand, within BWI76_RS17630 at 3662.360 kb on - strand, within BWI76_RS17630 at 3662.362 kb on + strand, within BWI76_RS17630 at 3662.362 kb on + strand, within BWI76_RS17630 at 3662.365 kb on - strand, within BWI76_RS17630 at 3662.420 kb on - strand, within BWI76_RS17630 at 3662.420 kb on - strand, within BWI76_RS17630 at 3662.423 kb on + strand, within BWI76_RS17630 at 3662.442 kb on + strand, within BWI76_RS17630 at 3662.442 kb on + strand, within BWI76_RS17630 at 3662.442 kb on + strand, within BWI76_RS17630 at 3662.442 kb on + strand, within BWI76_RS17630 at 3662.442 kb on + strand, within BWI76_RS17630 at 3662.442 kb on + strand, within BWI76_RS17630 at 3662.442 kb on + strand, within BWI76_RS17630 at 3662.450 kb on - strand, within BWI76_RS17630 at 3662.450 kb on - strand, within BWI76_RS17630 at 3662.466 kb on + strand, within BWI76_RS17630 at 3662.466 kb on + strand, within BWI76_RS17630 at 3662.466 kb on + strand, within BWI76_RS17630 at 3662.497 kb on - strand, within BWI76_RS17630 at 3662.554 kb on - strand, within BWI76_RS17630 at 3662.557 kb on + strand, within BWI76_RS17630 at 3662.565 kb on - strand, within BWI76_RS17630 at 3662.565 kb on - strand, within BWI76_RS17630 at 3662.568 kb on + strand, within BWI76_RS17630 at 3662.568 kb on + strand, within BWI76_RS17630 at 3662.568 kb on + strand, within BWI76_RS17630 at 3662.568 kb on + strand, within BWI76_RS17630 at 3662.568 kb on + strand, within BWI76_RS17630 at 3662.568 kb on + strand, within BWI76_RS17630 at 3662.575 kb on - strand, within BWI76_RS17630 at 3662.576 kb on - strand, within BWI76_RS17630 at 3662.576 kb on - strand, within BWI76_RS17630 at 3662.576 kb on - strand, within BWI76_RS17630 at 3662.576 kb on - strand, within BWI76_RS17630 at 3662.576 kb on - strand, within BWI76_RS17630 at 3662.576 kb on - strand, within BWI76_RS17630 at 3662.576 kb on - strand, within BWI76_RS17630 at 3662.587 kb on - strand at 3662.602 kb on - strand, within BWI76_RS17630 at 3662.602 kb on - strand, within BWI76_RS17630 at 3662.604 kb on + strand, within BWI76_RS17630 at 3662.609 kb on - strand, within BWI76_RS17630
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 3,659,675 - +0.5 3,659,733 + -0.0 3,659,938 + BWI76_RS17615 0.39 -0.3 3,660,052 + BWI76_RS17615 0.55 -1.2 3,660,222 + BWI76_RS17615 0.80 +0.9 3,660,298 + +1.5 3,660,360 - -0.1 3,660,387 + +0.4 3,660,476 + -0.0 3,660,476 + -0.7 3,660,476 + +0.7 3,660,477 + -1.1 3,660,484 + -0.4 3,660,485 + -0.3 3,660,490 + -0.2 3,660,490 + +0.3 3,660,495 + -0.3 3,660,501 - +0.4 3,660,607 - +0.1 3,660,628 - -1.7 3,660,638 + -0.0 3,660,683 - BWI76_RS17620 0.13 -1.0 3,660,698 - BWI76_RS17620 0.15 -3.2 3,660,728 + BWI76_RS17620 0.17 -0.1 3,660,736 - BWI76_RS17620 0.18 -0.9 3,661,137 - BWI76_RS17620 0.55 -0.9 3,661,172 - BWI76_RS17620 0.58 -0.1 3,661,335 + BWI76_RS17620 0.73 -0.0 3,661,670 - +0.4 3,661,687 - BWI76_RS17625 0.11 -0.2 3,661,969 + BWI76_RS17625 0.56 -0.7 3,662,070 - BWI76_RS17625 0.72 -0.5 3,662,121 - BWI76_RS17625 0.80 +1.1 3,662,132 + BWI76_RS17625 0.81 -0.4 3,662,148 + BWI76_RS17625 0.84 +0.4 3,662,171 - BWI76_RS17625 0.88 +1.1 3,662,171 - BWI76_RS17625 0.88 -0.0 3,662,171 - BWI76_RS17625 0.88 -0.3 3,662,171 - BWI76_RS17625 0.88 -0.1 3,662,175 + BWI76_RS17625 0.88 -0.1 3,662,180 + BWI76_RS17625 0.89 -2.3 3,662,180 + +1.5 3,662,183 - BWI76_RS17625 0.90 +0.1 3,662,188 - -0.0 3,662,189 - -1.0 3,662,200 - -1.0 3,662,226 + +1.3 3,662,226 + +0.3 3,662,226 + +0.4 3,662,226 + +0.1 3,662,226 + +0.6 3,662,226 + -1.1 3,662,226 + -0.5 3,662,226 + -0.3 3,662,234 - +0.2 3,662,234 - -0.6 3,662,234 - -0.9 3,662,234 - +0.9 3,662,234 - -0.1 3,662,234 - +0.2 3,662,247 + +0.1 3,662,266 + -0.5 3,662,292 + -0.3 3,662,295 - -0.4 3,662,305 - -0.1 3,662,352 + BWI76_RS17630 0.16 -0.5 3,662,355 - BWI76_RS17630 0.16 +0.7 3,662,355 - BWI76_RS17630 0.16 -0.1 3,662,360 - BWI76_RS17630 0.17 -3.5 3,662,360 - BWI76_RS17630 0.17 -3.7 3,662,362 + BWI76_RS17630 0.17 +0.1 3,662,362 + BWI76_RS17630 0.17 +0.4 3,662,365 - BWI76_RS17630 0.17 +0.1 3,662,420 - BWI76_RS17630 0.26 +0.3 3,662,420 - BWI76_RS17630 0.26 +0.1 3,662,423 + BWI76_RS17630 0.26 +0.3 3,662,442 + BWI76_RS17630 0.29 -0.7 3,662,442 + BWI76_RS17630 0.29 +0.0 3,662,442 + BWI76_RS17630 0.29 +0.4 3,662,442 + BWI76_RS17630 0.29 +0.6 3,662,442 + BWI76_RS17630 0.29 +0.0 3,662,442 + BWI76_RS17630 0.29 +0.4 3,662,442 + BWI76_RS17630 0.29 +0.2 3,662,450 - BWI76_RS17630 0.30 +0.5 3,662,450 - BWI76_RS17630 0.30 -1.1 3,662,466 + BWI76_RS17630 0.33 -0.7 3,662,466 + BWI76_RS17630 0.33 +0.6 3,662,466 + BWI76_RS17630 0.33 -0.2 3,662,497 - BWI76_RS17630 0.37 +1.2 3,662,554 - BWI76_RS17630 0.46 +0.4 3,662,557 + BWI76_RS17630 0.46 +0.1 3,662,565 - BWI76_RS17630 0.47 +0.2 3,662,565 - BWI76_RS17630 0.47 -0.9 3,662,568 + BWI76_RS17630 0.48 -0.4 3,662,568 + BWI76_RS17630 0.48 -0.1 3,662,568 + BWI76_RS17630 0.48 +1.2 3,662,568 + BWI76_RS17630 0.48 -0.2 3,662,568 + BWI76_RS17630 0.48 -0.8 3,662,568 + BWI76_RS17630 0.48 -0.3 3,662,575 - BWI76_RS17630 0.49 +1.2 3,662,576 - BWI76_RS17630 0.49 +0.0 3,662,576 - BWI76_RS17630 0.49 +0.2 3,662,576 - BWI76_RS17630 0.49 -2.2 3,662,576 - BWI76_RS17630 0.49 +0.5 3,662,576 - BWI76_RS17630 0.49 +0.0 3,662,576 - BWI76_RS17630 0.49 -0.1 3,662,576 - BWI76_RS17630 0.49 +0.4 3,662,587 - +2.5 3,662,602 - BWI76_RS17630 0.53 -0.9 3,662,602 - BWI76_RS17630 0.53 +0.6 3,662,604 + BWI76_RS17630 0.53 +0.1 3,662,609 - BWI76_RS17630 0.54 +0.1
Or see this region's nucleotide sequence