Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04860

Experiment: D-Alanine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04850 and BWI76_RS04855 are separated by 9 nucleotidesBWI76_RS04855 and BWI76_RS04860 are separated by 31 nucleotidesBWI76_RS04860 and BWI76_RS04865 overlap by 1 nucleotides BWI76_RS04850: BWI76_RS04850 - N-acetylmuramoyl-L-alanine amidase, at 1,013,104 to 1,013,655 _RS04850 BWI76_RS04855: BWI76_RS04855 - regulatory protein AmpE, at 1,013,665 to 1,014,519 _RS04855 BWI76_RS04860: BWI76_RS04860 - alpha-N-arabinofuranosidase, at 1,014,551 to 1,015,501 _RS04860 BWI76_RS04865: BWI76_RS04865 - MFS transporter, at 1,015,501 to 1,016,907 _RS04865 Position (kb) 1014 1015 1016Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1013.613 kb on - strandat 1013.675 kb on + strandat 1013.681 kb on - strandat 1013.708 kb on + strandat 1013.716 kb on - strandat 1013.716 kb on - strandat 1013.760 kb on - strand, within BWI76_RS04855at 1013.784 kb on + strand, within BWI76_RS04855at 1013.844 kb on + strand, within BWI76_RS04855at 1013.904 kb on + strand, within BWI76_RS04855at 1013.904 kb on - strand, within BWI76_RS04855at 1013.917 kb on + strandat 1014.080 kb on - strand, within BWI76_RS04855at 1014.278 kb on + strand, within BWI76_RS04855at 1014.395 kb on - strand, within BWI76_RS04855at 1014.681 kb on + strand, within BWI76_RS04860at 1014.735 kb on + strand, within BWI76_RS04860at 1014.748 kb on - strand, within BWI76_RS04860at 1014.824 kb on - strand, within BWI76_RS04860at 1014.841 kb on - strand, within BWI76_RS04860at 1015.293 kb on - strand, within BWI76_RS04860at 1015.293 kb on - strand, within BWI76_RS04860at 1015.366 kb on + strand, within BWI76_RS04860at 1015.374 kb on - strand, within BWI76_RS04860at 1015.400 kb on + strand, within BWI76_RS04860at 1015.493 kb on + strandat 1015.562 kb on - strandat 1015.683 kb on + strand, within BWI76_RS04865at 1015.683 kb on - strand, within BWI76_RS04865at 1015.885 kb on + strand, within BWI76_RS04865at 1015.893 kb on - strandat 1015.893 kb on - strand, within BWI76_RS04865at 1015.893 kb on - strand, within BWI76_RS04865at 1015.893 kb on - strand, within BWI76_RS04865at 1016.033 kb on - strand, within BWI76_RS04865at 1016.093 kb on - strand, within BWI76_RS04865at 1016.093 kb on - strand, within BWI76_RS04865at 1016.132 kb on + strand, within BWI76_RS04865at 1016.149 kb on - strand, within BWI76_RS04865at 1016.274 kb on - strandat 1016.367 kb on - strand, within BWI76_RS04865at 1016.380 kb on - strand, within BWI76_RS04865

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Alanine (C)
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1,013,613 - -2.8
1,013,675 + +0.3
1,013,681 - -0.3
1,013,708 + -0.4
1,013,716 - +1.2
1,013,716 - -1.2
1,013,760 - BWI76_RS04855 0.11 +0.2
1,013,784 + BWI76_RS04855 0.14 +0.1
1,013,844 + BWI76_RS04855 0.21 -0.3
1,013,904 + BWI76_RS04855 0.28 +0.5
1,013,904 - BWI76_RS04855 0.28 +2.3
1,013,917 + +0.3
1,014,080 - BWI76_RS04855 0.49 +0.1
1,014,278 + BWI76_RS04855 0.72 +0.0
1,014,395 - BWI76_RS04855 0.85 -0.7
1,014,681 + BWI76_RS04860 0.14 +2.1
1,014,735 + BWI76_RS04860 0.19 +0.6
1,014,748 - BWI76_RS04860 0.21 +0.0
1,014,824 - BWI76_RS04860 0.29 -0.6
1,014,841 - BWI76_RS04860 0.30 -0.6
1,015,293 - BWI76_RS04860 0.78 +0.0
1,015,293 - BWI76_RS04860 0.78 -0.0
1,015,366 + BWI76_RS04860 0.86 +0.2
1,015,374 - BWI76_RS04860 0.87 +0.2
1,015,400 + BWI76_RS04860 0.89 +1.9
1,015,493 + +0.3
1,015,562 - +0.3
1,015,683 + BWI76_RS04865 0.13 -0.3
1,015,683 - BWI76_RS04865 0.13 -0.7
1,015,885 + BWI76_RS04865 0.27 -0.6
1,015,893 - -0.1
1,015,893 - BWI76_RS04865 0.28 -0.6
1,015,893 - BWI76_RS04865 0.28 -1.2
1,015,893 - BWI76_RS04865 0.28 +0.1
1,016,033 - BWI76_RS04865 0.38 +0.8
1,016,093 - BWI76_RS04865 0.42 -0.4
1,016,093 - BWI76_RS04865 0.42 -0.3
1,016,132 + BWI76_RS04865 0.45 +0.6
1,016,149 - BWI76_RS04865 0.46 +0.7
1,016,274 - +0.3
1,016,367 - BWI76_RS04865 0.62 -0.6
1,016,380 - BWI76_RS04865 0.62 +1.3

Or see this region's nucleotide sequence