Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25730 and AO356_25735 are separated by 367 nucleotides AO356_25735 and AO356_25740 are separated by 82 nucleotides AO356_25740 and AO356_25745 overlap by 11 nucleotides AO356_25745 and AO356_25750 are separated by 2 nucleotides
AO356_25730: AO356_25730 - ABC transporter substrate-binding protein, at 5,819,568 to 5,820,593
_25730
AO356_25735: AO356_25735 - 3,4-dihydroxy-2-butanone 4-phosphate synthase, at 5,820,961 to 5,822,070
_25735
AO356_25740: AO356_25740 - hypothetical protein, at 5,822,153 to 5,822,455
_25740
AO356_25745: AO356_25745 - NUDIX hydrolase, at 5,822,445 to 5,823,008
_25745
AO356_25750: AO356_25750 - GntR family transcriptional regulator, at 5,823,011 to 5,823,763
_25750
Position (kb)
5820
5821
5822
5823 Strain fitness (log2 ratio)
-1
0
1
2 at 5820.109 kb on + strand, within AO356_25730 at 5820.110 kb on - strand, within AO356_25730 at 5820.196 kb on + strand, within AO356_25730 at 5820.217 kb on + strand, within AO356_25730 at 5820.218 kb on - strand, within AO356_25730 at 5820.218 kb on - strand, within AO356_25730 at 5820.656 kb on + strand at 5820.657 kb on - strand at 5820.657 kb on - strand at 5820.674 kb on - strand at 5820.698 kb on + strand at 5820.698 kb on + strand at 5820.801 kb on + strand at 5820.802 kb on - strand at 5821.100 kb on + strand, within AO356_25735 at 5821.271 kb on + strand, within AO356_25735 at 5821.272 kb on - strand, within AO356_25735 at 5821.272 kb on - strand, within AO356_25735 at 5821.468 kb on - strand, within AO356_25735 at 5821.693 kb on - strand, within AO356_25735 at 5821.693 kb on - strand, within AO356_25735 at 5822.153 kb on - strand at 5822.163 kb on - strand at 5822.201 kb on + strand, within AO356_25740 at 5822.210 kb on + strand, within AO356_25740 at 5822.211 kb on - strand, within AO356_25740 at 5822.242 kb on - strand, within AO356_25740 at 5822.242 kb on - strand, within AO356_25740 at 5822.244 kb on - strand, within AO356_25740 at 5822.244 kb on - strand, within AO356_25740 at 5822.244 kb on - strand, within AO356_25740 at 5822.336 kb on + strand, within AO356_25740 at 5822.358 kb on - strand, within AO356_25740 at 5822.372 kb on + strand, within AO356_25740 at 5822.489 kb on - strand at 5822.489 kb on - strand at 5822.489 kb on - strand at 5822.489 kb on - strand at 5822.489 kb on - strand at 5822.497 kb on + strand at 5822.498 kb on - strand at 5822.522 kb on + strand, within AO356_25745 at 5822.524 kb on + strand, within AO356_25745 at 5822.524 kb on + strand, within AO356_25745 at 5822.557 kb on + strand, within AO356_25745 at 5822.557 kb on + strand, within AO356_25745 at 5822.557 kb on + strand, within AO356_25745 at 5822.558 kb on - strand, within AO356_25745 at 5822.558 kb on - strand, within AO356_25745 at 5822.662 kb on + strand, within AO356_25745 at 5822.662 kb on + strand, within AO356_25745 at 5822.662 kb on + strand, within AO356_25745 at 5822.662 kb on + strand, within AO356_25745 at 5822.662 kb on + strand, within AO356_25745 at 5822.663 kb on - strand, within AO356_25745 at 5822.663 kb on - strand, within AO356_25745 at 5822.663 kb on - strand, within AO356_25745 at 5822.722 kb on - strand, within AO356_25745 at 5822.845 kb on + strand, within AO356_25745 at 5822.905 kb on + strand, within AO356_25745
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM remove 5,820,109 + AO356_25730 0.53 +0.3 5,820,110 - AO356_25730 0.53 +0.5 5,820,196 + AO356_25730 0.61 -0.4 5,820,217 + AO356_25730 0.63 -0.2 5,820,218 - AO356_25730 0.63 +0.4 5,820,218 - AO356_25730 0.63 -0.2 5,820,656 + +0.1 5,820,657 - +0.2 5,820,657 - +0.4 5,820,674 - +0.6 5,820,698 + -0.7 5,820,698 + +0.3 5,820,801 + +2.4 5,820,802 - +0.1 5,821,100 + AO356_25735 0.13 +0.1 5,821,271 + AO356_25735 0.28 +0.0 5,821,272 - AO356_25735 0.28 +0.3 5,821,272 - AO356_25735 0.28 +0.2 5,821,468 - AO356_25735 0.46 +0.0 5,821,693 - AO356_25735 0.66 +0.0 5,821,693 - AO356_25735 0.66 +0.4 5,822,153 - -0.6 5,822,163 - +0.7 5,822,201 + AO356_25740 0.16 -0.5 5,822,210 + AO356_25740 0.19 +0.6 5,822,211 - AO356_25740 0.19 -0.7 5,822,242 - AO356_25740 0.29 -1.0 5,822,242 - AO356_25740 0.29 -0.2 5,822,244 - AO356_25740 0.30 +0.6 5,822,244 - AO356_25740 0.30 +0.3 5,822,244 - AO356_25740 0.30 -0.2 5,822,336 + AO356_25740 0.60 +0.1 5,822,358 - AO356_25740 0.68 -0.2 5,822,372 + AO356_25740 0.72 +0.6 5,822,489 - -0.7 5,822,489 - -0.1 5,822,489 - +0.5 5,822,489 - -0.1 5,822,489 - +0.5 5,822,497 + +0.3 5,822,498 - -1.4 5,822,522 + AO356_25745 0.14 -0.2 5,822,524 + AO356_25745 0.14 +1.2 5,822,524 + AO356_25745 0.14 -0.6 5,822,557 + AO356_25745 0.20 +0.1 5,822,557 + AO356_25745 0.20 -0.7 5,822,557 + AO356_25745 0.20 -0.2 5,822,558 - AO356_25745 0.20 +1.0 5,822,558 - AO356_25745 0.20 -0.4 5,822,662 + AO356_25745 0.38 +0.2 5,822,662 + AO356_25745 0.38 +0.1 5,822,662 + AO356_25745 0.38 +0.8 5,822,662 + AO356_25745 0.38 -1.0 5,822,662 + AO356_25745 0.38 -1.4 5,822,663 - AO356_25745 0.39 -1.7 5,822,663 - AO356_25745 0.39 -0.4 5,822,663 - AO356_25745 0.39 -0.0 5,822,722 - AO356_25745 0.49 -0.0 5,822,845 + AO356_25745 0.71 +1.1 5,822,905 + AO356_25745 0.82 +0.3
Or see this region's nucleotide sequence