Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3625 and Shew_3626 are separated by 302 nucleotides Shew_3626 and Shew_3627 overlap by 4 nucleotides Shew_3627 and Shew_3628 are separated by 156 nucleotides Shew_3628 and Shew_3629 overlap by 7 nucleotides
Shew_3625: Shew_3625 - sodium:dicarboxylate symporter (RefSeq), at 4,324,508 to 4,325,734
_3625
Shew_3626: Shew_3626 - hypothetical protein (RefSeq), at 4,326,037 to 4,326,462
_3626
Shew_3627: Shew_3627 - XRE family transcriptional regulator (RefSeq), at 4,326,459 to 4,326,656
_3627
Shew_3628: Shew_3628 - MgtC/SapB transporter (RefSeq), at 4,326,813 to 4,327,295
_3628
Shew_3629: Shew_3629 - competence protein ComF (RefSeq), at 4,327,289 to 4,328,047
_3629
Position (kb)
4326
4327 Strain fitness (log2 ratio)
-1
0
1
2
3 at 4325.804 kb on - strand at 4325.918 kb on - strand at 4326.070 kb on - strand at 4326.121 kb on - strand, within Shew_3626 at 4326.163 kb on + strand, within Shew_3626 at 4326.266 kb on + strand, within Shew_3626 at 4326.651 kb on + strand at 4326.917 kb on - strand, within Shew_3628 at 4326.969 kb on - strand, within Shew_3628 at 4327.073 kb on + strand, within Shew_3628 at 4327.525 kb on + strand, within Shew_3629
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 4,325,804 - -0.6 4,325,918 - +0.5 4,326,070 - +0.4 4,326,121 - Shew_3626 0.20 +0.3 4,326,163 + Shew_3626 0.30 -1.1 4,326,266 + Shew_3626 0.54 +0.4 4,326,651 + +0.4 4,326,917 - Shew_3628 0.22 -0.5 4,326,969 - Shew_3628 0.32 +0.4 4,327,073 + Shew_3628 0.54 +3.7 4,327,525 + Shew_3629 0.31 +0.6
Or see this region's nucleotide sequence