Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3588 and Shew_3589 are separated by 110 nucleotides Shew_3589 and Shew_3590 are separated by 141 nucleotides Shew_3590 and Shew_3591 overlap by 11 nucleotides
Shew_3588: Shew_3588 - Bcr/CflA subfamily drug resistance transporter (RefSeq), at 4,285,833 to 4,287,035
_3588
Shew_3589: Shew_3589 - LysR family transcriptional regulator (RefSeq), at 4,287,146 to 4,288,138
_3589
Shew_3590: Shew_3590 - hypothetical protein (RefSeq), at 4,288,280 to 4,288,630
_3590
Shew_3591: Shew_3591 - hypothetical protein (RefSeq), at 4,288,620 to 4,289,939
_3591
Position (kb) 4287
4288
4289 Strain fitness (log2 ratio) -2
-1
0
1 at 4286.152 kb on + strand, within Shew_3588 at 4287.178 kb on + strand at 4287.864 kb on - strand, within Shew_3589 at 4287.964 kb on - strand, within Shew_3589 at 4288.003 kb on + strand, within Shew_3589 at 4288.015 kb on + strand, within Shew_3589 at 4288.023 kb on - strand, within Shew_3589 at 4288.072 kb on + strand at 4288.092 kb on - strand at 4288.592 kb on + strand, within Shew_3590 at 4288.681 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 4,286,152 + Shew_3588 0.27 -1.0 4,287,178 + -0.4 4,287,864 - Shew_3589 0.72 +0.4 4,287,964 - Shew_3589 0.82 -0.2 4,288,003 + Shew_3589 0.86 -0.3 4,288,015 + Shew_3589 0.88 +0.2 4,288,023 - Shew_3589 0.88 -0.2 4,288,072 + +0.0 4,288,092 - +0.4 4,288,592 + Shew_3590 0.89 +1.1 4,288,681 - -2.1
Or see this region's nucleotide sequence