Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2663 and Shew_2664 are separated by 398 nucleotides Shew_2664 and Shew_2665 overlap by 1 nucleotides
Shew_2663: Shew_2663 - elongation factor G (RefSeq), at 3,136,916 to 3,138,964
_2663
Shew_2664: Shew_2664 - transcriptional regulator, putative (RefSeq), at 3,139,363 to 3,140,217
_2664
Shew_2665: Shew_2665 - beta-ketoacyl synthase (RefSeq), at 3,140,217 to 3,148,076
_2665
Position (kb)
3139
3140
3141 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3138.404 kb on - strand, within Shew_2663 at 3138.582 kb on + strand, within Shew_2663 at 3138.582 kb on + strand, within Shew_2663 at 3138.582 kb on + strand, within Shew_2663 at 3138.582 kb on + strand, within Shew_2663 at 3138.582 kb on + strand, within Shew_2663 at 3138.590 kb on - strand, within Shew_2663 at 3138.590 kb on - strand, within Shew_2663 at 3138.590 kb on - strand, within Shew_2663 at 3138.775 kb on + strand at 3138.775 kb on + strand at 3138.775 kb on + strand at 3138.783 kb on - strand at 3138.783 kb on - strand at 3138.797 kb on - strand at 3138.900 kb on - strand at 3139.093 kb on - strand at 3139.147 kb on - strand at 3139.610 kb on - strand, within Shew_2664 at 3139.659 kb on + strand, within Shew_2664 at 3139.683 kb on + strand, within Shew_2664 at 3139.709 kb on + strand, within Shew_2664 at 3139.920 kb on - strand, within Shew_2664 at 3139.942 kb on - strand, within Shew_2664 at 3140.157 kb on + strand at 3140.544 kb on - strand at 3141.009 kb on + strand, within Shew_2665
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 3,138,404 - Shew_2663 0.73 +0.1 3,138,582 + Shew_2663 0.81 +0.2 3,138,582 + Shew_2663 0.81 -0.3 3,138,582 + Shew_2663 0.81 +0.1 3,138,582 + Shew_2663 0.81 -0.3 3,138,582 + Shew_2663 0.81 -0.4 3,138,590 - Shew_2663 0.82 -0.1 3,138,590 - Shew_2663 0.82 -0.6 3,138,590 - Shew_2663 0.82 +0.3 3,138,775 + +0.4 3,138,775 + +1.3 3,138,775 + -0.2 3,138,783 - +0.4 3,138,783 - +0.4 3,138,797 - +0.1 3,138,900 - +0.4 3,139,093 - -0.0 3,139,147 - -0.4 3,139,610 - Shew_2664 0.29 -4.0 3,139,659 + Shew_2664 0.35 -1.5 3,139,683 + Shew_2664 0.37 -1.1 3,139,709 + Shew_2664 0.40 -3.0 3,139,920 - Shew_2664 0.65 -3.5 3,139,942 - Shew_2664 0.68 -1.0 3,140,157 + -2.7 3,140,544 - -5.1 3,141,009 + Shew_2665 0.10 -4.5
Or see this region's nucleotide sequence