Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2542 and Shew_2543 are separated by 133 nucleotides Shew_2543 and Shew_2544 are separated by 168 nucleotides Shew_2544 and Shew_2545 are separated by 233 nucleotides
Shew_2542: Shew_2542 - transcription activator, effector binding (RefSeq), at 2,998,654 to 2,999,112
_2542
Shew_2543: Shew_2543 - glyoxalase family protein (RefSeq), at 2,999,246 to 2,999,665
_2543
Shew_2544: Shew_2544 - phosphoglucomutase/phosphomannomutase alpha/beta/subunit (RefSeq), at 2,999,834 to 3,001,588
_2544
Shew_2545: Shew_2545 - diguanylate cyclase (RefSeq), at 3,001,822 to 3,002,727
_2545
Position (kb)
2999
3000
3001
3002 Strain fitness (log2 ratio)
-1
0
1 at 2998.842 kb on + strand, within Shew_2542 at 2998.959 kb on - strand, within Shew_2542 at 2999.014 kb on - strand, within Shew_2542 at 2999.410 kb on + strand, within Shew_2543 at 2999.411 kb on - strand, within Shew_2543 at 2999.571 kb on + strand, within Shew_2543 at 2999.612 kb on + strand, within Shew_2543 at 2999.612 kb on + strand, within Shew_2543 at 2999.703 kb on - strand at 2999.703 kb on - strand at 2999.756 kb on - strand at 2999.897 kb on + strand at 3000.473 kb on - strand, within Shew_2544 at 3000.825 kb on - strand, within Shew_2544 at 3001.347 kb on + strand, within Shew_2544 at 3001.806 kb on - strand at 3001.920 kb on + strand, within Shew_2545 at 3001.939 kb on - strand, within Shew_2545 at 3001.941 kb on + strand, within Shew_2545 at 3002.086 kb on + strand, within Shew_2545 at 3002.150 kb on + strand, within Shew_2545 at 3002.439 kb on - strand, within Shew_2545
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 2,998,842 + Shew_2542 0.41 +0.2 2,998,959 - Shew_2542 0.66 +0.4 2,999,014 - Shew_2542 0.78 +0.4 2,999,410 + Shew_2543 0.39 -0.3 2,999,411 - Shew_2543 0.39 +0.9 2,999,571 + Shew_2543 0.77 +0.1 2,999,612 + Shew_2543 0.87 +0.7 2,999,612 + Shew_2543 0.87 -0.3 2,999,703 - +0.4 2,999,703 - -0.7 2,999,756 - -0.1 2,999,897 + +0.3 3,000,473 - Shew_2544 0.36 -0.6 3,000,825 - Shew_2544 0.56 -1.2 3,001,347 + Shew_2544 0.86 -0.5 3,001,806 - +0.3 3,001,920 + Shew_2545 0.11 -0.3 3,001,939 - Shew_2545 0.13 -0.1 3,001,941 + Shew_2545 0.13 +0.8 3,002,086 + Shew_2545 0.29 -0.8 3,002,150 + Shew_2545 0.36 -0.4 3,002,439 - Shew_2545 0.68 -0.3
Or see this region's nucleotide sequence