Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2492 and Shew_2493 are separated by 85 nucleotides Shew_2493 and Shew_2494 are separated by 89 nucleotides Shew_2494 and pspB overlap by 14 nucleotides pspB and Shew_2496 are separated by 20 nucleotides
Shew_2492: Shew_2492 - hypothetical protein (RefSeq), at 2,931,878 to 2,932,972
_2492
Shew_2493: Shew_2493 - protein of unknown function DUF463, YcjX family protein (RefSeq), at 2,933,058 to 2,934,503
_2493
Shew_2494: Shew_2494 - phage shock protein C, PspC (RefSeq), at 2,934,593 to 2,934,988
_2494
Shew_2495: pspB - phage shock protein B (RefSeq), at 2,934,975 to 2,935,214
pspB
Shew_2496: Shew_2496 - phage shock protein A, PspA (RefSeq), at 2,935,235 to 2,935,918
_2496
Position (kb)
2933
2934
2935 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 2932.564 kb on + strand, within Shew_2492 at 2932.572 kb on - strand, within Shew_2492 at 2932.572 kb on - strand, within Shew_2492 at 2932.572 kb on - strand, within Shew_2492 at 2932.657 kb on + strand, within Shew_2492 at 2932.665 kb on - strand, within Shew_2492 at 2933.176 kb on - strand at 2933.266 kb on - strand, within Shew_2493 at 2933.655 kb on + strand, within Shew_2493 at 2934.148 kb on + strand, within Shew_2493 at 2934.187 kb on - strand, within Shew_2493 at 2934.524 kb on + strand at 2934.613 kb on + strand at 2934.636 kb on + strand, within Shew_2494 at 2934.926 kb on + strand, within Shew_2494 at 2935.299 kb on - strand at 2935.360 kb on + strand, within Shew_2496
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 2,932,564 + Shew_2492 0.63 -0.4 2,932,572 - Shew_2492 0.63 +0.2 2,932,572 - Shew_2492 0.63 -0.8 2,932,572 - Shew_2492 0.63 -1.2 2,932,657 + Shew_2492 0.71 -0.8 2,932,665 - Shew_2492 0.72 -1.1 2,933,176 - -0.9 2,933,266 - Shew_2493 0.14 -0.6 2,933,655 + Shew_2493 0.41 -1.2 2,934,148 + Shew_2493 0.75 -1.3 2,934,187 - Shew_2493 0.78 -0.6 2,934,524 + -1.0 2,934,613 + -6.4 2,934,636 + Shew_2494 0.11 -5.8 2,934,926 + Shew_2494 0.84 -6.9 2,935,299 - -7.3 2,935,360 + Shew_2496 0.18 -2.0
Or see this region's nucleotide sequence