Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2224 and Shew_2225 are separated by 310 nucleotides Shew_2225 and Shew_2226 are separated by 638 nucleotides
Shew_2224: Shew_2224 - DNA topoisomerase I (RefSeq), at 2,594,700 to 2,597,396
_2224
Shew_2225: Shew_2225 - succinylarginine dihydrolase (RefSeq), at 2,597,707 to 2,599,044
_2225
Shew_2226: Shew_2226 - Ig domain-containing protein (RefSeq), at 2,599,683 to 2,602,142
_2226
Position (kb)
2597
2598
2599
2600 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2597.546 kb on - strand at 2597.653 kb on + strand at 2597.653 kb on + strand at 2597.663 kb on + strand at 2597.739 kb on + strand at 2597.994 kb on + strand, within Shew_2225 at 2597.994 kb on + strand, within Shew_2225 at 2597.994 kb on + strand, within Shew_2225 at 2598.002 kb on - strand, within Shew_2225 at 2598.002 kb on - strand, within Shew_2225 at 2598.002 kb on - strand, within Shew_2225 at 2598.002 kb on - strand, within Shew_2225 at 2598.052 kb on - strand, within Shew_2225 at 2598.130 kb on - strand, within Shew_2225 at 2598.312 kb on + strand, within Shew_2225 at 2598.351 kb on + strand, within Shew_2225 at 2598.502 kb on + strand, within Shew_2225 at 2598.985 kb on + strand at 2599.357 kb on - strand at 2599.362 kb on + strand at 2599.370 kb on - strand at 2599.370 kb on - strand at 2599.559 kb on + strand at 2599.582 kb on + strand at 2599.734 kb on - strand at 2599.795 kb on + strand at 2599.810 kb on + strand at 2599.916 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 2,597,546 - -1.2 2,597,653 + -0.4 2,597,653 + -0.1 2,597,663 + +0.9 2,597,739 + +0.1 2,597,994 + Shew_2225 0.21 +0.4 2,597,994 + Shew_2225 0.21 -0.2 2,597,994 + Shew_2225 0.21 +0.6 2,598,002 - Shew_2225 0.22 +0.1 2,598,002 - Shew_2225 0.22 -0.1 2,598,002 - Shew_2225 0.22 +0.8 2,598,002 - Shew_2225 0.22 +0.2 2,598,052 - Shew_2225 0.26 +0.7 2,598,130 - Shew_2225 0.32 -2.9 2,598,312 + Shew_2225 0.45 +0.1 2,598,351 + Shew_2225 0.48 -2.5 2,598,502 + Shew_2225 0.59 -0.6 2,598,985 + +0.1 2,599,357 - +0.2 2,599,362 + +0.1 2,599,370 - -0.1 2,599,370 - +0.8 2,599,559 + +0.2 2,599,582 + +0.1 2,599,734 - +0.3 2,599,795 + -0.1 2,599,810 + -0.4 2,599,916 - -0.3
Or see this region's nucleotide sequence