Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2161 and cpdB are separated by 305 nucleotides cpdB and Shew_2163 are separated by 123 nucleotides Shew_2163 and Shew_2164 are separated by 112 nucleotides
Shew_2161: Shew_2161 - peptidyl-dipeptidase Dcp (RefSeq), at 2,508,721 to 2,510,901
_2161
Shew_2162: cpdB - bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein (RefSeq), at 2,511,207 to 2,513,291
cpdB
Shew_2163: Shew_2163 - hypothetical protein (RefSeq), at 2,513,415 to 2,514,017
_2163
Shew_2164: Shew_2164 - acyl-CoA dehydrogenase domain-containing protein (RefSeq), at 2,514,130 to 2,515,920
_2164
Position (kb)
2511
2512
2513
2514 Strain fitness (log2 ratio)
-1
0
1 at 2510.258 kb on - strand, within Shew_2161 at 2510.879 kb on + strand at 2511.101 kb on + strand at 2511.109 kb on - strand at 2511.952 kb on - strand, within cpdB at 2512.021 kb on - strand, within cpdB at 2512.162 kb on + strand, within cpdB at 2512.322 kb on - strand, within cpdB at 2512.343 kb on + strand, within cpdB at 2512.494 kb on + strand, within cpdB at 2512.862 kb on + strand, within cpdB at 2513.165 kb on - strand at 2513.952 kb on + strand, within Shew_2163 at 2514.210 kb on - strand at 2514.291 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 2,510,258 - Shew_2161 0.70 +0.2 2,510,879 + +0.5 2,511,101 + +0.3 2,511,109 - -0.2 2,511,952 - cpdB Shew_2162 0.36 +1.4 2,512,021 - cpdB Shew_2162 0.39 +0.4 2,512,162 + cpdB Shew_2162 0.46 +0.9 2,512,322 - cpdB Shew_2162 0.53 -0.2 2,512,343 + cpdB Shew_2162 0.54 -0.5 2,512,494 + cpdB Shew_2162 0.62 +0.3 2,512,862 + cpdB Shew_2162 0.79 +0.2 2,513,165 - +0.2 2,513,952 + Shew_2163 0.89 +1.4 2,514,210 - -0.0 2,514,291 + +0.7
Or see this region's nucleotide sequence