Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1810 and Shew_1811 are separated by 159 nucleotides Shew_1811 and Shew_R0057 are separated by 814 nucleotides Shew_R0057 and Shew_R0058 are separated by 46 nucleotides
Shew_1810: Shew_1810 - dihydroorotate dehydrogenase 2 (RefSeq), at 2,079,784 to 2,080,809
_1810
Shew_1811: Shew_1811 - hypothetical protein (RefSeq), at 2,080,969 to 2,081,502
_1811
Shew_R0057: Shew_R0057 - tRNA-Met (RefSeq), at 2,082,317 to 2,082,393
_R0057
Shew_R0058: Shew_R0058 - tRNA-Met (RefSeq), at 2,082,440 to 2,082,516
_R0058
Position (kb)
2080
2081
2082 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2080.161 kb on + strand, within Shew_1810 at 2080.658 kb on + strand, within Shew_1810 at 2080.666 kb on - strand, within Shew_1810 at 2080.944 kb on + strand at 2080.952 kb on + strand at 2080.952 kb on + strand at 2080.960 kb on - strand at 2080.960 kb on - strand at 2081.192 kb on + strand, within Shew_1811 at 2081.192 kb on + strand, within Shew_1811 at 2081.192 kb on + strand, within Shew_1811 at 2081.200 kb on - strand, within Shew_1811 at 2081.200 kb on - strand, within Shew_1811 at 2081.200 kb on - strand, within Shew_1811 at 2081.200 kb on - strand, within Shew_1811 at 2081.293 kb on + strand, within Shew_1811 at 2081.438 kb on + strand, within Shew_1811 at 2081.438 kb on + strand, within Shew_1811 at 2081.438 kb on + strand, within Shew_1811 at 2081.499 kb on - strand at 2081.770 kb on - strand at 2081.813 kb on + strand at 2081.813 kb on + strand at 2081.821 kb on + strand at 2081.821 kb on + strand at 2081.821 kb on - strand at 2081.821 kb on + strand at 2081.939 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 2,080,161 + Shew_1810 0.37 +3.3 2,080,658 + Shew_1810 0.85 +3.1 2,080,666 - Shew_1810 0.86 +3.1 2,080,944 + -0.7 2,080,952 + -1.3 2,080,952 + -0.5 2,080,960 - -1.1 2,080,960 - -1.2 2,081,192 + Shew_1811 0.42 -2.1 2,081,192 + Shew_1811 0.42 -1.5 2,081,192 + Shew_1811 0.42 -0.8 2,081,200 - Shew_1811 0.43 -1.5 2,081,200 - Shew_1811 0.43 -2.2 2,081,200 - Shew_1811 0.43 -1.1 2,081,200 - Shew_1811 0.43 -1.8 2,081,293 + Shew_1811 0.61 -1.4 2,081,438 + Shew_1811 0.88 -0.9 2,081,438 + Shew_1811 0.88 -1.1 2,081,438 + Shew_1811 0.88 -1.0 2,081,499 - -0.6 2,081,770 - +0.0 2,081,813 + -0.4 2,081,813 + +0.0 2,081,821 + +0.1 2,081,821 + +0.3 2,081,821 - +0.5 2,081,821 + +0.5 2,081,939 - +0.5
Or see this region's nucleotide sequence