Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1715 and Shew_1716 are separated by 6 nucleotides Shew_1716 and Shew_1717 are separated by 30 nucleotides Shew_1717 and Shew_1718 are separated by 163 nucleotides
Shew_1715: Shew_1715 - peptide synthase (RefSeq), at 1,967,988 to 1,969,679
_1715
Shew_1716: Shew_1716 - 3-beta hydroxysteroid dehydrogenase/isomerase (RefSeq), at 1,969,686 to 1,970,807
_1716
Shew_1717: Shew_1717 - globin (RefSeq), at 1,970,838 to 1,971,260
_1717
Shew_1718: Shew_1718 - adenosylmethionine-8-amino-7-oxononanoate aminotransferase (RefSeq), at 1,971,424 to 1,972,764
_1718
Position (kb) 1969
1970
1971 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1968.970 kb on - strand, within Shew_1715 at 1970.054 kb on + strand, within Shew_1716 at 1970.346 kb on + strand, within Shew_1716 at 1970.808 kb on + strand at 1970.958 kb on + strand, within Shew_1717 at 1971.001 kb on + strand, within Shew_1717 at 1971.141 kb on + strand, within Shew_1717 at 1971.263 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 1,968,970 - Shew_1715 0.58 -2.7 1,970,054 + Shew_1716 0.33 -2.6 1,970,346 + Shew_1716 0.59 -4.5 1,970,808 + +0.1 1,970,958 + Shew_1717 0.28 +0.0 1,971,001 + Shew_1717 0.39 +0.2 1,971,141 + Shew_1717 0.72 -2.6 1,971,263 - +0.3
Or see this region's nucleotide sequence