Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1679 and Shew_1680 are separated by 128 nucleotides Shew_1680 and Shew_1681 are separated by 218 nucleotides Shew_1681 and Shew_1682 are separated by 42 nucleotides Shew_1682 and Shew_1683 are separated by 30 nucleotides
Shew_1679: Shew_1679 - TetR family transcriptional regulator (RefSeq), at 1,930,027 to 1,930,584
_1679
Shew_1680: Shew_1680 - HPP family protein+B94 (RefSeq), at 1,930,713 to 1,931,183
_1680
Shew_1681: Shew_1681 - diguanylate cyclase with PAS/PAC sensor (RefSeq), at 1,931,402 to 1,932,892
_1681
Shew_1682: Shew_1682 - ribonuclease H (RefSeq), at 1,932,935 to 1,933,699
_1682
Shew_1683: Shew_1683 - hypothetical protein (RefSeq), at 1,933,730 to 1,934,305
_1683
Position (kb) 1931
1932
1933 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1930.689 kb on + strand at 1931.364 kb on + strand at 1931.420 kb on - strand at 1931.776 kb on + strand, within Shew_1681 at 1932.603 kb on - strand, within Shew_1681 at 1933.016 kb on + strand, within Shew_1682 at 1933.258 kb on + strand, within Shew_1682
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 1,930,689 + +0.2 1,931,364 + -0.4 1,931,420 - +0.3 1,931,776 + Shew_1681 0.25 -0.4 1,932,603 - Shew_1681 0.81 -4.3 1,933,016 + Shew_1682 0.11 +0.6 1,933,258 + Shew_1682 0.42 -0.4
Or see this region's nucleotide sequence