Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1279 and Shew_1280 are separated by 279 nucleotides Shew_1280 and Shew_1281 overlap by 41 nucleotides Shew_1281 and Shew_1282 are separated by 617 nucleotides Shew_1282 and Shew_1283 are separated by 127 nucleotides
Shew_1279: Shew_1279 - hypothetical protein (RefSeq), at 1,479,636 to 1,480,460
_1279
Shew_1280: Shew_1280 - CopG family transcriptional regulator (RefSeq), at 1,480,740 to 1,481,165
_1280
Shew_1281: Shew_1281 - hypothetical protein (RefSeq), at 1,481,125 to 1,481,868
_1281
Shew_1282: Shew_1282 - hypothetical protein (RefSeq), at 1,482,486 to 1,482,710
_1282
Shew_1283: Shew_1283 - NrfJ-related protein (RefSeq), at 1,482,838 to 1,483,140
_1283
Position (kb)
1481
1482 Strain fitness (log2 ratio)
-2
-1
0
1 at 1480.426 kb on + strand at 1480.441 kb on + strand at 1480.614 kb on - strand at 1480.641 kb on + strand at 1480.750 kb on + strand at 1480.889 kb on + strand, within Shew_1280 at 1480.897 kb on - strand, within Shew_1280 at 1480.897 kb on - strand, within Shew_1280 at 1481.122 kb on + strand, within Shew_1280 at 1481.122 kb on + strand, within Shew_1280 at 1481.414 kb on + strand, within Shew_1281 at 1481.640 kb on - strand, within Shew_1281 at 1482.082 kb on + strand at 1482.464 kb on - strand at 1482.648 kb on + strand, within Shew_1282 at 1482.817 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 1,480,426 + -0.1 1,480,441 + +0.1 1,480,614 - +0.3 1,480,641 + +0.2 1,480,750 + +0.7 1,480,889 + Shew_1280 0.35 +0.1 1,480,897 - Shew_1280 0.37 +0.4 1,480,897 - Shew_1280 0.37 +0.6 1,481,122 + Shew_1280 0.90 -0.5 1,481,122 + Shew_1280 0.90 +0.4 1,481,414 + Shew_1281 0.39 +0.1 1,481,640 - Shew_1281 0.69 -1.9 1,482,082 + +0.0 1,482,464 - -0.0 1,482,648 + Shew_1282 0.72 -0.1 1,482,817 - +0.4
Or see this region's nucleotide sequence