Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1069 and Shew_1070 are separated by 59 nucleotides Shew_1070 and Shew_1071 are separated by 250 nucleotides Shew_1071 and Shew_1072 are separated by 76 nucleotides
Shew_1069: Shew_1069 - hydroxylamine reductase (RefSeq), at 1,230,817 to 1,232,481
_1069
Shew_1070: Shew_1070 - oxidoreductase FAD-binding subunit (RefSeq), at 1,232,541 to 1,233,626
_1070
Shew_1071: Shew_1071 - hypothetical protein (RefSeq), at 1,233,877 to 1,234,212
_1071
Shew_1072: Shew_1072 - chorismate mutase (RefSeq), at 1,234,289 to 1,236,253
_1072
Position (kb)
1232
1233
1234 Strain fitness (log2 ratio)
-1
0
1 at 1231.687 kb on + strand, within Shew_1069 at 1231.938 kb on + strand, within Shew_1069 at 1232.100 kb on - strand, within Shew_1069 at 1232.113 kb on + strand, within Shew_1069 at 1232.184 kb on + strand, within Shew_1069 at 1232.695 kb on - strand, within Shew_1070 at 1232.764 kb on + strand, within Shew_1070 at 1233.055 kb on - strand, within Shew_1070 at 1233.083 kb on + strand, within Shew_1070 at 1233.184 kb on - strand, within Shew_1070 at 1233.417 kb on - strand, within Shew_1070 at 1233.479 kb on + strand, within Shew_1070 at 1233.480 kb on - strand, within Shew_1070 at 1233.564 kb on - strand at 1233.783 kb on + strand at 1233.922 kb on - strand, within Shew_1071 at 1234.108 kb on - strand, within Shew_1071 at 1234.357 kb on + strand at 1234.371 kb on + strand at 1234.439 kb on + strand at 1234.473 kb on - strand at 1234.541 kb on - strand, within Shew_1072 at 1234.605 kb on - strand, within Shew_1072
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 1,231,687 + Shew_1069 0.52 -0.1 1,231,938 + Shew_1069 0.67 +0.0 1,232,100 - Shew_1069 0.77 -0.0 1,232,113 + Shew_1069 0.78 +0.8 1,232,184 + Shew_1069 0.82 +0.5 1,232,695 - Shew_1070 0.14 -0.8 1,232,764 + Shew_1070 0.21 -0.3 1,233,055 - Shew_1070 0.47 +0.4 1,233,083 + Shew_1070 0.50 -0.2 1,233,184 - Shew_1070 0.59 -0.3 1,233,417 - Shew_1070 0.81 +0.0 1,233,479 + Shew_1070 0.86 +0.3 1,233,480 - Shew_1070 0.86 -0.9 1,233,564 - -0.1 1,233,783 + +0.2 1,233,922 - Shew_1071 0.13 +0.5 1,234,108 - Shew_1071 0.69 +0.5 1,234,357 + +0.3 1,234,371 + +1.0 1,234,439 + +1.6 1,234,473 - -0.2 1,234,541 - Shew_1072 0.13 +0.1 1,234,605 - Shew_1072 0.16 -0.5
Or see this region's nucleotide sequence