Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0614 and Shew_0615 are separated by 154 nucleotides Shew_0615 and Shew_0616 are separated by 331 nucleotides
Shew_0614: Shew_0614 - cysteine/glutathione ABC transporter membrane/ATP-binding component (RefSeq), at 695,749 to 697,524
_0614
Shew_0615: Shew_0615 - bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase (RefSeq), at 697,679 to 700,858
_0615
Shew_0616: Shew_0616 - SH3 domain-containing protein (RefSeq), at 701,190 to 701,738
_0616
Position (kb) 697
698
699
700
701 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 696.774 kb on - strand, within Shew_0614 at 697.106 kb on - strand, within Shew_0614 at 697.141 kb on + strand, within Shew_0614 at 697.151 kb on - strand, within Shew_0614 at 697.229 kb on - strand, within Shew_0614 at 697.365 kb on + strand at 698.809 kb on + strand, within Shew_0615 at 699.170 kb on + strand, within Shew_0615 at 699.512 kb on + strand, within Shew_0615 at 699.857 kb on - strand, within Shew_0615 at 699.879 kb on + strand, within Shew_0615 at 699.900 kb on + strand, within Shew_0615 at 699.944 kb on + strand, within Shew_0615 at 700.227 kb on + strand, within Shew_0615 at 700.447 kb on - strand, within Shew_0615 at 700.734 kb on + strand at 700.742 kb on - strand at 700.742 kb on - strand at 700.742 kb on - strand at 700.749 kb on + strand at 701.245 kb on - strand, within Shew_0616 at 701.453 kb on - strand, within Shew_0616 at 701.476 kb on + strand, within Shew_0616 at 701.517 kb on + strand, within Shew_0616 at 701.633 kb on + strand, within Shew_0616
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 696,774 - Shew_0614 0.58 +2.7 697,106 - Shew_0614 0.76 +1.4 697,141 + Shew_0614 0.78 +0.6 697,151 - Shew_0614 0.79 +0.3 697,229 - Shew_0614 0.83 +1.9 697,365 + +1.3 698,809 + Shew_0615 0.36 +1.5 699,170 + Shew_0615 0.47 +1.6 699,512 + Shew_0615 0.58 +1.0 699,857 - Shew_0615 0.68 +1.2 699,879 + Shew_0615 0.69 +1.5 699,900 + Shew_0615 0.70 +0.3 699,944 + Shew_0615 0.71 +1.4 700,227 + Shew_0615 0.80 +1.7 700,447 - Shew_0615 0.87 +1.1 700,734 + +1.2 700,742 - +1.3 700,742 - +0.6 700,742 - +1.4 700,749 + +2.5 701,245 - Shew_0616 0.10 +0.1 701,453 - Shew_0616 0.48 -0.3 701,476 + Shew_0616 0.52 -0.0 701,517 + Shew_0616 0.60 -4.1 701,633 + Shew_0616 0.81 -1.0
Or see this region's nucleotide sequence