Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0298 and Shew_0299 are separated by 164 nucleotides Shew_0299 and Shew_0300 are separated by 130 nucleotides
Shew_0298: Shew_0298 - Zn-dependent hydrolase of the beta-lactamase fold (RefSeq), at 319,961 to 321,049
_0298
Shew_0299: Shew_0299 - TetR family transcriptional regulator (RefSeq), at 321,214 to 321,822
_0299
Shew_0300: Shew_0300 - sodium:neurotransmitter symporter (RefSeq), at 321,953 to 323,311
_0300
Position (kb)
321
322 Strain fitness (log2 ratio)
-1
0
1 at 320.230 kb on - strand, within Shew_0298 at 320.337 kb on - strand, within Shew_0298 at 320.463 kb on + strand, within Shew_0298 at 320.513 kb on + strand, within Shew_0298 at 320.773 kb on - strand, within Shew_0298 at 320.778 kb on + strand, within Shew_0298 at 320.791 kb on - strand, within Shew_0298 at 320.960 kb on - strand at 321.034 kb on + strand at 321.251 kb on - strand at 321.387 kb on + strand, within Shew_0299 at 321.528 kb on - strand, within Shew_0299 at 321.629 kb on + strand, within Shew_0299 at 321.725 kb on + strand, within Shew_0299 at 321.762 kb on + strand at 321.782 kb on + strand at 321.825 kb on - strand at 321.888 kb on - strand at 322.088 kb on + strand at 322.217 kb on - strand, within Shew_0300 at 322.468 kb on - strand, within Shew_0300 at 322.664 kb on + strand, within Shew_0300 at 322.672 kb on - strand, within Shew_0300
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 320,230 - Shew_0298 0.25 +0.0 320,337 - Shew_0298 0.35 +0.0 320,463 + Shew_0298 0.46 -0.5 320,513 + Shew_0298 0.51 -0.8 320,773 - Shew_0298 0.75 -0.4 320,778 + Shew_0298 0.75 +0.2 320,791 - Shew_0298 0.76 +0.1 320,960 - +0.2 321,034 + +0.4 321,251 - -0.3 321,387 + Shew_0299 0.28 +0.5 321,528 - Shew_0299 0.52 +1.3 321,629 + Shew_0299 0.68 +0.8 321,725 + Shew_0299 0.84 +0.6 321,762 + +0.7 321,782 + +1.3 321,825 - +0.1 321,888 - +0.1 322,088 + +0.6 322,217 - Shew_0300 0.19 -0.2 322,468 - Shew_0300 0.38 -0.2 322,664 + Shew_0300 0.52 -0.6 322,672 - Shew_0300 0.53 +0.1
Or see this region's nucleotide sequence