Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0059 and Shew_0060 are separated by 27 nucleotides Shew_0060 and Shew_0061 are separated by 50 nucleotides
Shew_0059: Shew_0059 - molybdopterin oxidoreductase (RefSeq), at 69,872 to 72,721
_0059
Shew_0060: Shew_0060 - 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein (RefSeq), at 72,749 to 73,339
_0060
Shew_0061: Shew_0061 - formate dehydrogenase, gamma subunit (RefSeq), at 73,390 to 74,397
_0061
Position (kb) 72
73
74 Strain fitness (log2 ratio) -1
0
1
2 at 71.953 kb on + strand, within Shew_0059 at 71.970 kb on - strand, within Shew_0059 at 72.064 kb on + strand, within Shew_0059 at 72.122 kb on - strand, within Shew_0059 at 72.192 kb on - strand, within Shew_0059 at 72.204 kb on - strand, within Shew_0059 at 72.204 kb on - strand, within Shew_0059 at 72.261 kb on + strand, within Shew_0059 at 72.261 kb on + strand, within Shew_0059 at 72.266 kb on + strand, within Shew_0059 at 72.269 kb on - strand, within Shew_0059 at 72.271 kb on + strand, within Shew_0059 at 72.372 kb on - strand, within Shew_0059 at 72.415 kb on + strand, within Shew_0059 at 72.556 kb on - strand at 72.614 kb on - strand at 72.629 kb on - strand at 72.644 kb on + strand at 72.987 kb on + strand, within Shew_0060 at 72.987 kb on + strand, within Shew_0060 at 72.987 kb on + strand, within Shew_0060 at 72.995 kb on - strand, within Shew_0060 at 73.003 kb on - strand, within Shew_0060 at 73.133 kb on + strand, within Shew_0060 at 73.141 kb on - strand, within Shew_0060 at 73.206 kb on + strand, within Shew_0060 at 73.365 kb on - strand at 73.374 kb on + strand at 73.638 kb on + strand, within Shew_0061 at 73.828 kb on + strand, within Shew_0061 at 73.836 kb on - strand, within Shew_0061 at 73.853 kb on - strand, within Shew_0061 at 73.988 kb on + strand, within Shew_0061 at 73.996 kb on - strand, within Shew_0061 at 74.048 kb on - strand, within Shew_0061 at 74.119 kb on - strand, within Shew_0061 at 74.119 kb on - strand, within Shew_0061 at 74.130 kb on + strand, within Shew_0061 at 74.295 kb on - strand, within Shew_0061 at 74.330 kb on - strand at 74.330 kb on - strand at 74.332 kb on + strand at 74.332 kb on + strand at 74.332 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 71,953 + Shew_0059 0.73 +0.4 71,970 - Shew_0059 0.74 +0.3 72,064 + Shew_0059 0.77 +0.5 72,122 - Shew_0059 0.79 +0.6 72,192 - Shew_0059 0.81 +1.1 72,204 - Shew_0059 0.82 +0.9 72,204 - Shew_0059 0.82 +0.6 72,261 + Shew_0059 0.84 +0.5 72,261 + Shew_0059 0.84 +0.6 72,266 + Shew_0059 0.84 +0.6 72,269 - Shew_0059 0.84 +0.6 72,271 + Shew_0059 0.84 +0.5 72,372 - Shew_0059 0.88 +1.5 72,415 + Shew_0059 0.89 +1.0 72,556 - +2.4 72,614 - +0.4 72,629 - +1.2 72,644 + +0.5 72,987 + Shew_0060 0.40 +0.1 72,987 + Shew_0060 0.40 +1.7 72,987 + Shew_0060 0.40 +0.8 72,995 - Shew_0060 0.42 +0.8 73,003 - Shew_0060 0.43 +1.0 73,133 + Shew_0060 0.65 +0.6 73,141 - Shew_0060 0.66 +0.6 73,206 + Shew_0060 0.77 +1.0 73,365 - +0.6 73,374 + +0.9 73,638 + Shew_0061 0.25 -0.3 73,828 + Shew_0061 0.43 +0.6 73,836 - Shew_0061 0.44 +1.0 73,853 - Shew_0061 0.46 -0.1 73,988 + Shew_0061 0.59 +0.5 73,996 - Shew_0061 0.60 +0.7 74,048 - Shew_0061 0.65 +0.8 74,119 - Shew_0061 0.72 +0.4 74,119 - Shew_0061 0.72 +0.2 74,130 + Shew_0061 0.73 +0.4 74,295 - Shew_0061 0.90 +0.6 74,330 - +2.4 74,330 - -0.1 74,332 + +0.5 74,332 + +0.7 74,332 + +2.0
Or see this region's nucleotide sequence