Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0056 and Shew_0057 are separated by 10 nucleotides Shew_0057 and Shew_0058 are separated by 167 nucleotides Shew_0058 and Shew_0059 are separated by 51 nucleotides
Shew_0056: Shew_0056 - 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein (RefSeq), at 67,100 to 68,776
_0056
Shew_0057: Shew_0057 - cytoplasmic chaperone TorD family protein (RefSeq), at 68,787 to 69,452
_0057
Shew_0058: Shew_0058 - twin-arginine translocation pathway signal (RefSeq), at 69,620 to 69,820
_0058
Shew_0059: Shew_0059 - molybdopterin oxidoreductase (RefSeq), at 69,872 to 72,721
_0059
Position (kb) 69
70 Strain fitness (log2 ratio) -1
0
1 at 68.688 kb on + strand at 68.825 kb on + strand at 68.825 kb on + strand at 68.991 kb on - strand, within Shew_0057 at 69.146 kb on + strand, within Shew_0057 at 69.329 kb on + strand, within Shew_0057 at 69.472 kb on - strand at 69.506 kb on + strand at 69.638 kb on + strand at 69.671 kb on + strand, within Shew_0058 at 69.671 kb on + strand, within Shew_0058 at 69.679 kb on - strand, within Shew_0058 at 69.679 kb on - strand, within Shew_0058 at 69.679 kb on - strand, within Shew_0058 at 69.679 kb on - strand, within Shew_0058 at 69.681 kb on + strand, within Shew_0058 at 69.757 kb on + strand, within Shew_0058 at 69.855 kb on + strand at 69.863 kb on - strand at 69.934 kb on - strand at 70.083 kb on + strand at 70.367 kb on - strand, within Shew_0059 at 70.381 kb on + strand, within Shew_0059 at 70.419 kb on - strand, within Shew_0059 at 70.601 kb on + strand, within Shew_0059
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 68,688 + -0.1 68,825 + +0.5 68,825 + +1.6 68,991 - Shew_0057 0.31 +0.3 69,146 + Shew_0057 0.54 +0.7 69,329 + Shew_0057 0.81 -0.5 69,472 - +1.2 69,506 + +0.5 69,638 + +0.1 69,671 + Shew_0058 0.25 +0.5 69,671 + Shew_0058 0.25 +1.5 69,679 - Shew_0058 0.29 +0.5 69,679 - Shew_0058 0.29 +0.6 69,679 - Shew_0058 0.29 +0.7 69,679 - Shew_0058 0.29 +1.2 69,681 + Shew_0058 0.30 +0.8 69,757 + Shew_0058 0.68 +0.6 69,855 + +0.4 69,863 - +0.4 69,934 - +0.6 70,083 + +0.7 70,367 - Shew_0059 0.17 +0.4 70,381 + Shew_0059 0.18 +0.3 70,419 - Shew_0059 0.19 +0.3 70,601 + Shew_0059 0.26 +1.1
Or see this region's nucleotide sequence