Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0053 and Shew_0054 are separated by 295 nucleotides Shew_0054 and Shew_0055 overlap by 1 nucleotides Shew_0055 and Shew_0056 are separated by 181 nucleotides
Shew_0053: Shew_0053 - formate dehydrogenase, subunit FdhD (RefSeq), at 64,717 to 65,562
_0053
Shew_0054: Shew_0054 - hypothetical protein (RefSeq), at 65,858 to 66,328
_0054
Shew_0055: Shew_0055 - hypothetical protein (RefSeq), at 66,328 to 66,918
_0055
Shew_0056: Shew_0056 - 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein (RefSeq), at 67,100 to 68,776
_0056
Position (kb) 66
67 Strain fitness (log2 ratio) -1
0
1
2 at 65.394 kb on + strand, within Shew_0053 at 65.528 kb on + strand at 65.536 kb on - strand at 65.594 kb on + strand at 65.720 kb on - strand at 65.991 kb on + strand, within Shew_0054 at 66.021 kb on - strand, within Shew_0054 at 66.063 kb on - strand, within Shew_0054 at 66.178 kb on - strand, within Shew_0054 at 66.258 kb on + strand, within Shew_0054 at 66.291 kb on - strand at 66.362 kb on + strand at 66.486 kb on + strand, within Shew_0055 at 66.545 kb on + strand, within Shew_0055 at 66.553 kb on - strand, within Shew_0055 at 66.615 kb on + strand, within Shew_0055 at 66.621 kb on - strand, within Shew_0055 at 66.673 kb on + strand, within Shew_0055 at 66.678 kb on + strand, within Shew_0055 at 66.683 kb on + strand, within Shew_0055 at 66.686 kb on - strand, within Shew_0055 at 67.030 kb on - strand at 67.207 kb on + strand at 67.269 kb on - strand, within Shew_0056 at 67.284 kb on + strand, within Shew_0056 at 67.715 kb on - strand, within Shew_0056 at 67.824 kb on + strand, within Shew_0056 at 67.832 kb on - strand, within Shew_0056 at 67.832 kb on - strand, within Shew_0056
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 65,394 + Shew_0053 0.80 +0.7 65,528 + +0.6 65,536 - +0.5 65,594 + +0.5 65,720 - -0.2 65,991 + Shew_0054 0.28 +0.2 66,021 - Shew_0054 0.35 +0.8 66,063 - Shew_0054 0.44 -0.1 66,178 - Shew_0054 0.68 +0.6 66,258 + Shew_0054 0.85 -0.0 66,291 - +0.8 66,362 + +0.5 66,486 + Shew_0055 0.27 +0.6 66,545 + Shew_0055 0.37 +0.6 66,553 - Shew_0055 0.38 +0.4 66,615 + Shew_0055 0.49 +0.1 66,621 - Shew_0055 0.50 +1.0 66,673 + Shew_0055 0.58 +1.1 66,678 + Shew_0055 0.59 +0.6 66,683 + Shew_0055 0.60 +1.2 66,686 - Shew_0055 0.61 +0.7 67,030 - +1.1 67,207 + +0.7 67,269 - Shew_0056 0.10 +0.7 67,284 + Shew_0056 0.11 +0.5 67,715 - Shew_0056 0.37 +1.2 67,824 + Shew_0056 0.43 +0.6 67,832 - Shew_0056 0.44 +0.8 67,832 - Shew_0056 0.44 +2.0
Or see this region's nucleotide sequence