Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0020 and Shew_0021 are separated by 14 nucleotides Shew_0021 and Shew_0022 are separated by 152 nucleotides Shew_0022 and Shew_0023 overlap by 1 nucleotides Shew_0023 and Shew_0024 are separated by 21 nucleotides
Shew_0020: Shew_0020 - proline dipeptidase (RefSeq), at 23,495 to 24,817
_0020
Shew_0021: Shew_0021 - hypothetical protein (RefSeq), at 24,832 to 25,446
_0021
Shew_0022: Shew_0022 - TrkH family potassium uptake protein (RefSeq), at 25,599 to 27,056
_0022
Shew_0023: Shew_0023 - protoporphyrinogen oxidase (RefSeq), at 27,056 to 27,583
_0023
Shew_0024: Shew_0024 - ArsR family transcriptional regulator (RefSeq), at 27,605 to 27,910
_0024
Position (kb) 25
26
27
28 Strain fitness (log2 ratio) -2
-1
0
1
2 at 24.811 kb on - strand at 25.148 kb on + strand, within Shew_0021 at 25.223 kb on - strand, within Shew_0021 at 25.227 kb on + strand, within Shew_0021 at 25.283 kb on - strand, within Shew_0021 at 25.307 kb on + strand, within Shew_0021 at 25.365 kb on + strand, within Shew_0021 at 25.375 kb on + strand, within Shew_0021 at 25.375 kb on + strand, within Shew_0021 at 25.418 kb on - strand at 25.462 kb on - strand at 25.795 kb on + strand, within Shew_0022 at 25.823 kb on + strand, within Shew_0022 at 25.823 kb on + strand, within Shew_0022 at 25.933 kb on - strand, within Shew_0022 at 25.967 kb on + strand, within Shew_0022 at 26.182 kb on - strand, within Shew_0022 at 27.032 kb on - strand at 27.097 kb on + strand at 27.165 kb on + strand, within Shew_0023 at 27.173 kb on - strand, within Shew_0023 at 27.173 kb on - strand, within Shew_0023 at 27.208 kb on + strand, within Shew_0023 at 27.420 kb on - strand, within Shew_0023 at 27.540 kb on + strand at 27.556 kb on - strand at 27.567 kb on + strand at 27.594 kb on - strand at 27.671 kb on + strand, within Shew_0024 at 27.851 kb on - strand, within Shew_0024
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 24,811 - +0.0 25,148 + Shew_0021 0.51 -0.3 25,223 - Shew_0021 0.64 +0.2 25,227 + Shew_0021 0.64 -0.5 25,283 - Shew_0021 0.73 +0.2 25,307 + Shew_0021 0.77 +0.1 25,365 + Shew_0021 0.87 +0.3 25,375 + Shew_0021 0.88 -2.1 25,375 + Shew_0021 0.88 -1.5 25,418 - +1.8 25,462 - -0.0 25,795 + Shew_0022 0.13 +2.2 25,823 + Shew_0022 0.15 +2.5 25,823 + Shew_0022 0.15 +1.9 25,933 - Shew_0022 0.23 +2.3 25,967 + Shew_0022 0.25 +2.2 26,182 - Shew_0022 0.40 +2.7 27,032 - +2.1 27,097 + -0.2 27,165 + Shew_0023 0.21 +0.2 27,173 - Shew_0023 0.22 -0.3 27,173 - Shew_0023 0.22 -0.7 27,208 + Shew_0023 0.29 -0.4 27,420 - Shew_0023 0.69 +0.3 27,540 + -0.8 27,556 - -0.5 27,567 + -0.4 27,594 - +0.5 27,671 + Shew_0024 0.22 +0.2 27,851 - Shew_0024 0.80 +0.4
Or see this region's nucleotide sequence