Experiment: Urea (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3058 and Dshi_3059 are separated by 3 nucleotides Dshi_3059 and Dshi_3060 are separated by 78 nucleotides Dshi_3060 and Dshi_3061 are separated by 151 nucleotides Dshi_3061 and Dshi_3062 overlap by 4 nucleotides
Dshi_3058: Dshi_3058 - hypothetical protein (RefSeq), at 3,225,943 to 3,228,627
_3058
Dshi_3059: Dshi_3059 - diaminopimelate decarboxylase (RefSeq), at 3,228,631 to 3,229,926
_3059
Dshi_3060: Dshi_3060 - hypothetical protein (RefSeq), at 3,230,005 to 3,230,340
_3060
Dshi_3061: Dshi_3061 - hypothetical protein (RefSeq), at 3,230,492 to 3,230,662
_3061
Dshi_3062: Dshi_3062 - argininosuccinate lyase (RefSeq), at 3,230,659 to 3,232,050
_3062
Position (kb)
3228
3229
3230 Strain fitness (log2 ratio)
-1
0
1 at 3227.685 kb on - strand, within Dshi_3058 at 3227.768 kb on - strand, within Dshi_3058 at 3227.943 kb on - strand, within Dshi_3058 at 3228.014 kb on - strand, within Dshi_3058 at 3228.128 kb on - strand, within Dshi_3058 at 3228.138 kb on - strand, within Dshi_3058 at 3228.219 kb on - strand, within Dshi_3058 at 3228.293 kb on - strand, within Dshi_3058 at 3228.312 kb on - strand, within Dshi_3058 at 3228.362 kb on - strand at 3228.557 kb on - strand at 3228.574 kb on + strand at 3228.611 kb on + strand at 3228.629 kb on + strand at 3228.629 kb on + strand at 3228.654 kb on + strand at 3230.181 kb on + strand, within Dshi_3060 at 3230.316 kb on + strand at 3230.324 kb on - strand at 3230.478 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Urea (N) remove 3,227,685 - Dshi_3058 0.65 -1.0 3,227,768 - Dshi_3058 0.68 -0.0 3,227,943 - Dshi_3058 0.74 -0.2 3,228,014 - Dshi_3058 0.77 -0.1 3,228,128 - Dshi_3058 0.81 -0.7 3,228,138 - Dshi_3058 0.82 -0.3 3,228,219 - Dshi_3058 0.85 -0.3 3,228,293 - Dshi_3058 0.88 -0.6 3,228,312 - Dshi_3058 0.88 -0.6 3,228,362 - -0.7 3,228,557 - -0.5 3,228,574 + -1.3 3,228,611 + +0.3 3,228,629 + +0.9 3,228,629 + -0.4 3,228,654 + -0.8 3,230,181 + Dshi_3060 0.52 -0.3 3,230,316 + -0.8 3,230,324 - +0.1 3,230,478 + -0.5
Or see this region's nucleotide sequence