Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05155 and MMP_RS05160 are separated by 231 nucleotides MMP_RS05160 and MMP_RS05165 are separated by 297 nucleotides MMP_RS05165 and MMP_RS05170 are separated by 84 nucleotides MMP_RS05170 and MMP_RS05175 overlap by 4 nucleotides
MMP_RS05155: MMP_RS05155 - hypothetical protein, at 982,388 to 982,651
_RS05155
MMP_RS05160: MMP_RS05160 - cupredoxin family copper-binding protein, at 982,883 to 983,239
_RS05160
MMP_RS05165: MMP_RS05165 - right-handed parallel beta-helix repeat-containing protein, at 983,537 to 984,193
_RS05165
MMP_RS05170: MMP_RS05170 - lysine exporter LysO family protein, at 984,278 to 984,865
_RS05170
MMP_RS05175: MMP_RS05175 - hypothetical protein, at 984,862 to 985,110
_RS05175
Position (kb) 983
984
985 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 982.611 kb on + strand, within MMP_RS05155 at 982.611 kb on + strand, within MMP_RS05155 at 982.611 kb on + strand, within MMP_RS05155 at 982.666 kb on - strand at 982.667 kb on + strand at 982.667 kb on + strand at 982.727 kb on + strand at 982.773 kb on + strand at 982.830 kb on - strand at 982.852 kb on - strand at 982.874 kb on + strand at 982.914 kb on - strand at 982.915 kb on + strand at 982.928 kb on + strand, within MMP_RS05160 at 982.928 kb on + strand, within MMP_RS05160 at 982.928 kb on + strand, within MMP_RS05160 at 982.932 kb on - strand, within MMP_RS05160 at 982.936 kb on + strand, within MMP_RS05160 at 982.940 kb on + strand, within MMP_RS05160 at 982.940 kb on + strand, within MMP_RS05160 at 982.941 kb on - strand, within MMP_RS05160 at 982.942 kb on + strand, within MMP_RS05160 at 982.942 kb on + strand, within MMP_RS05160 at 982.942 kb on + strand, within MMP_RS05160 at 982.949 kb on - strand, within MMP_RS05160 at 982.963 kb on - strand, within MMP_RS05160 at 982.964 kb on + strand, within MMP_RS05160 at 983.007 kb on + strand, within MMP_RS05160 at 983.024 kb on - strand, within MMP_RS05160 at 983.024 kb on - strand, within MMP_RS05160 at 983.156 kb on + strand, within MMP_RS05160 at 983.217 kb on + strand at 983.219 kb on - strand at 983.220 kb on + strand at 983.220 kb on + strand at 983.265 kb on + strand at 983.265 kb on + strand at 983.285 kb on - strand at 983.291 kb on - strand at 983.313 kb on - strand at 983.314 kb on + strand at 983.319 kb on + strand at 983.324 kb on + strand at 983.341 kb on - strand at 983.342 kb on + strand at 983.412 kb on - strand at 983.583 kb on - strand at 983.595 kb on - strand at 983.646 kb on - strand, within MMP_RS05165 at 983.646 kb on - strand, within MMP_RS05165 at 983.646 kb on - strand, within MMP_RS05165 at 983.646 kb on - strand, within MMP_RS05165 at 983.647 kb on + strand, within MMP_RS05165 at 983.686 kb on - strand, within MMP_RS05165 at 983.686 kb on - strand, within MMP_RS05165 at 983.698 kb on + strand, within MMP_RS05165 at 983.698 kb on + strand, within MMP_RS05165 at 983.700 kb on - strand, within MMP_RS05165 at 983.701 kb on + strand, within MMP_RS05165 at 983.717 kb on - strand, within MMP_RS05165 at 983.754 kb on - strand, within MMP_RS05165 at 983.798 kb on + strand, within MMP_RS05165 at 983.801 kb on + strand, within MMP_RS05165 at 983.804 kb on + strand, within MMP_RS05165 at 983.826 kb on - strand, within MMP_RS05165 at 983.844 kb on - strand, within MMP_RS05165 at 983.977 kb on + strand, within MMP_RS05165 at 983.988 kb on - strand, within MMP_RS05165 at 983.990 kb on - strand, within MMP_RS05165 at 983.990 kb on - strand, within MMP_RS05165 at 983.991 kb on + strand, within MMP_RS05165 at 984.011 kb on - strand, within MMP_RS05165 at 984.011 kb on - strand, within MMP_RS05165 at 984.012 kb on + strand, within MMP_RS05165 at 984.012 kb on + strand, within MMP_RS05165 at 984.012 kb on + strand, within MMP_RS05165 at 984.012 kb on + strand, within MMP_RS05165 at 984.015 kb on - strand, within MMP_RS05165 at 984.015 kb on - strand, within MMP_RS05165 at 984.015 kb on - strand, within MMP_RS05165 at 984.015 kb on - strand, within MMP_RS05165 at 984.016 kb on + strand, within MMP_RS05165 at 984.016 kb on + strand, within MMP_RS05165 at 984.016 kb on + strand, within MMP_RS05165 at 984.017 kb on - strand, within MMP_RS05165 at 984.020 kb on + strand, within MMP_RS05165 at 984.020 kb on + strand, within MMP_RS05165 at 984.083 kb on - strand, within MMP_RS05165 at 984.084 kb on + strand, within MMP_RS05165 at 984.093 kb on - strand, within MMP_RS05165 at 984.093 kb on - strand, within MMP_RS05165 at 984.159 kb on - strand at 984.160 kb on + strand at 984.272 kb on - strand at 984.362 kb on - strand, within MMP_RS05170 at 984.478 kb on - strand, within MMP_RS05170 at 984.479 kb on + strand, within MMP_RS05170 at 984.479 kb on + strand, within MMP_RS05170 at 984.540 kb on - strand, within MMP_RS05170 at 984.667 kb on - strand, within MMP_RS05170 at 984.667 kb on - strand, within MMP_RS05170 at 984.766 kb on + strand, within MMP_RS05170 at 984.766 kb on + strand, within MMP_RS05170 at 984.805 kb on - strand, within MMP_RS05170 at 984.820 kb on - strand at 984.821 kb on + strand at 984.873 kb on - strand at 984.906 kb on - strand, within MMP_RS05175 at 984.914 kb on + strand, within MMP_RS05175 at 984.940 kb on - strand, within MMP_RS05175 at 984.941 kb on + strand, within MMP_RS05175 at 984.941 kb on + strand, within MMP_RS05175 at 985.079 kb on + strand, within MMP_RS05175 at 985.096 kb on + strand at 985.097 kb on - strand at 985.098 kb on + strand at 985.098 kb on + strand at 985.110 kb on - strand at 985.142 kb on - strand at 985.158 kb on + strand at 985.159 kb on - strand at 985.162 kb on - strand at 985.162 kb on - strand at 985.164 kb on - strand at 985.165 kb on + strand at 985.165 kb on + strand at 985.166 kb on - strand at 985.167 kb on + strand at 985.175 kb on + strand at 985.175 kb on + strand at 985.186 kb on - strand at 985.187 kb on + strand at 985.189 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 982,611 + MMP_RS05155 0.84 -0.1 982,611 + MMP_RS05155 0.84 +0.5 982,611 + MMP_RS05155 0.84 -0.4 982,666 - +0.1 982,667 + -0.4 982,667 + -0.1 982,727 + -0.5 982,773 + +0.2 982,830 - +1.2 982,852 - +0.8 982,874 + +0.8 982,914 - -0.2 982,915 + +0.2 982,928 + MMP_RS05160 0.13 +0.9 982,928 + MMP_RS05160 0.13 -0.3 982,928 + MMP_RS05160 0.13 -0.0 982,932 - MMP_RS05160 0.14 +1.2 982,936 + MMP_RS05160 0.15 -0.0 982,940 + MMP_RS05160 0.16 +0.9 982,940 + MMP_RS05160 0.16 +0.2 982,941 - MMP_RS05160 0.16 -0.5 982,942 + MMP_RS05160 0.17 -0.3 982,942 + MMP_RS05160 0.17 +0.3 982,942 + MMP_RS05160 0.17 -0.9 982,949 - MMP_RS05160 0.18 -0.4 982,963 - MMP_RS05160 0.22 -0.4 982,964 + MMP_RS05160 0.23 -0.3 983,007 + MMP_RS05160 0.35 -0.3 983,024 - MMP_RS05160 0.39 -0.4 983,024 - MMP_RS05160 0.39 +0.7 983,156 + MMP_RS05160 0.76 -0.2 983,217 + -2.4 983,219 - +0.0 983,220 + -0.0 983,220 + +0.1 983,265 + +2.0 983,265 + -0.2 983,285 - +0.1 983,291 - -0.0 983,313 - +0.4 983,314 + +1.4 983,319 + +1.6 983,324 + -0.4 983,341 - -0.3 983,342 + +0.8 983,412 - +0.2 983,583 - -0.3 983,595 - +0.1 983,646 - MMP_RS05165 0.17 +0.2 983,646 - MMP_RS05165 0.17 -0.4 983,646 - MMP_RS05165 0.17 -2.0 983,646 - MMP_RS05165 0.17 -1.0 983,647 + MMP_RS05165 0.17 -0.5 983,686 - MMP_RS05165 0.23 +2.2 983,686 - MMP_RS05165 0.23 -1.2 983,698 + MMP_RS05165 0.25 -0.1 983,698 + MMP_RS05165 0.25 -0.2 983,700 - MMP_RS05165 0.25 -0.3 983,701 + MMP_RS05165 0.25 -1.0 983,717 - MMP_RS05165 0.27 -0.1 983,754 - MMP_RS05165 0.33 -0.5 983,798 + MMP_RS05165 0.40 +0.1 983,801 + MMP_RS05165 0.40 -0.4 983,804 + MMP_RS05165 0.41 -0.5 983,826 - MMP_RS05165 0.44 -0.9 983,844 - MMP_RS05165 0.47 -0.5 983,977 + MMP_RS05165 0.67 +0.3 983,988 - MMP_RS05165 0.69 -0.0 983,990 - MMP_RS05165 0.69 -0.0 983,990 - MMP_RS05165 0.69 -0.2 983,991 + MMP_RS05165 0.69 -0.5 984,011 - MMP_RS05165 0.72 -0.2 984,011 - MMP_RS05165 0.72 -0.2 984,012 + MMP_RS05165 0.72 -1.6 984,012 + MMP_RS05165 0.72 -0.6 984,012 + MMP_RS05165 0.72 +0.1 984,012 + MMP_RS05165 0.72 -0.1 984,015 - MMP_RS05165 0.73 -1.2 984,015 - MMP_RS05165 0.73 +0.0 984,015 - MMP_RS05165 0.73 -0.1 984,015 - MMP_RS05165 0.73 -0.8 984,016 + MMP_RS05165 0.73 -0.2 984,016 + MMP_RS05165 0.73 +0.4 984,016 + MMP_RS05165 0.73 -0.4 984,017 - MMP_RS05165 0.73 -0.4 984,020 + MMP_RS05165 0.74 +1.1 984,020 + MMP_RS05165 0.74 +0.2 984,083 - MMP_RS05165 0.83 -0.2 984,084 + MMP_RS05165 0.83 -0.1 984,093 - MMP_RS05165 0.85 +0.2 984,093 - MMP_RS05165 0.85 +1.0 984,159 - -0.7 984,160 + -0.4 984,272 - +0.5 984,362 - MMP_RS05170 0.14 +0.1 984,478 - MMP_RS05170 0.34 -1.2 984,479 + MMP_RS05170 0.34 -1.2 984,479 + MMP_RS05170 0.34 -0.4 984,540 - MMP_RS05170 0.45 +2.8 984,667 - MMP_RS05170 0.66 +0.8 984,667 - MMP_RS05170 0.66 +0.1 984,766 + MMP_RS05170 0.83 -1.0 984,766 + MMP_RS05170 0.83 +1.5 984,805 - MMP_RS05170 0.90 -1.3 984,820 - -0.1 984,821 + +0.9 984,873 - -0.6 984,906 - MMP_RS05175 0.18 +0.4 984,914 + MMP_RS05175 0.21 +1.0 984,940 - MMP_RS05175 0.31 -0.5 984,941 + MMP_RS05175 0.32 -0.3 984,941 + MMP_RS05175 0.32 -0.2 985,079 + MMP_RS05175 0.87 +0.3 985,096 + +0.1 985,097 - -0.0 985,098 + -0.0 985,098 + -1.0 985,110 - -0.2 985,142 - -0.4 985,158 + +1.0 985,159 - +0.2 985,162 - +1.2 985,162 - -0.4 985,164 - -1.0 985,165 + -0.6 985,165 + +0.0 985,166 - -1.0 985,167 + -0.0 985,175 + +2.5 985,175 + -0.1 985,186 - -0.2 985,187 + +0.4 985,189 + +0.7
Or see this region's nucleotide sequence