Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04230 and MMP_RS04235 are separated by 334 nucleotides MMP_RS04235 and MMP_RS04240 are separated by 132 nucleotides
MMP_RS04230: MMP_RS04230 - hypothetical protein, at 803,702 to 805,855
_RS04230
MMP_RS04235: MMP_RS04235 - hypothetical protein, at 806,190 to 807,299
_RS04235
MMP_RS04240: MMP_RS04240 - hypothetical protein, at 807,432 to 808,670
_RS04240
Position (kb) 806
807
808 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 805.200 kb on + strand, within MMP_RS04230 at 805.200 kb on + strand, within MMP_RS04230 at 805.234 kb on + strand, within MMP_RS04230 at 805.255 kb on - strand, within MMP_RS04230 at 805.305 kb on + strand, within MMP_RS04230 at 805.311 kb on + strand, within MMP_RS04230 at 805.316 kb on + strand, within MMP_RS04230 at 805.319 kb on - strand, within MMP_RS04230 at 805.320 kb on + strand, within MMP_RS04230 at 805.320 kb on + strand, within MMP_RS04230 at 805.320 kb on + strand, within MMP_RS04230 at 805.321 kb on - strand, within MMP_RS04230 at 805.321 kb on - strand, within MMP_RS04230 at 805.322 kb on + strand, within MMP_RS04230 at 805.322 kb on + strand, within MMP_RS04230 at 805.322 kb on + strand, within MMP_RS04230 at 805.352 kb on - strand, within MMP_RS04230 at 805.353 kb on + strand, within MMP_RS04230 at 805.353 kb on + strand, within MMP_RS04230 at 805.357 kb on - strand, within MMP_RS04230 at 805.358 kb on + strand, within MMP_RS04230 at 805.362 kb on - strand, within MMP_RS04230 at 805.362 kb on - strand, within MMP_RS04230 at 805.363 kb on + strand, within MMP_RS04230 at 805.363 kb on + strand, within MMP_RS04230 at 805.406 kb on - strand, within MMP_RS04230 at 805.452 kb on - strand, within MMP_RS04230 at 805.452 kb on - strand, within MMP_RS04230 at 805.452 kb on - strand, within MMP_RS04230 at 805.453 kb on + strand, within MMP_RS04230 at 805.454 kb on - strand, within MMP_RS04230 at 805.537 kb on + strand, within MMP_RS04230 at 805.541 kb on + strand, within MMP_RS04230 at 805.551 kb on + strand, within MMP_RS04230 at 805.551 kb on + strand, within MMP_RS04230 at 805.585 kb on - strand, within MMP_RS04230 at 805.644 kb on - strand at 805.645 kb on + strand at 805.648 kb on - strand at 805.706 kb on + strand at 805.761 kb on - strand at 805.761 kb on - strand at 805.761 kb on - strand at 805.761 kb on - strand at 805.762 kb on + strand at 805.776 kb on + strand at 805.777 kb on - strand at 805.777 kb on - strand at 805.811 kb on - strand at 805.873 kb on + strand at 805.887 kb on + strand at 805.891 kb on - strand at 805.892 kb on + strand at 805.892 kb on + strand at 805.892 kb on + strand at 805.895 kb on - strand at 805.895 kb on - strand at 805.895 kb on - strand at 805.895 kb on - strand at 805.895 kb on - strand at 805.895 kb on - strand at 805.895 kb on - strand at 805.895 kb on - strand at 805.896 kb on + strand at 805.896 kb on + strand at 805.897 kb on - strand at 805.909 kb on + strand at 805.916 kb on + strand at 805.916 kb on + strand at 805.922 kb on - strand at 805.927 kb on - strand at 805.928 kb on + strand at 805.966 kb on - strand at 805.967 kb on + strand at 805.970 kb on - strand at 805.971 kb on + strand at 805.978 kb on + strand at 806.007 kb on - strand at 806.007 kb on - strand at 806.016 kb on + strand at 806.028 kb on + strand at 806.050 kb on - strand at 806.050 kb on - strand at 806.055 kb on + strand at 806.114 kb on - strand at 806.115 kb on + strand at 806.115 kb on + strand at 806.120 kb on - strand at 806.138 kb on - strand at 806.139 kb on + strand at 806.142 kb on - strand at 806.155 kb on - strand at 806.158 kb on - strand at 806.159 kb on + strand at 806.166 kb on + strand at 806.167 kb on - strand at 806.168 kb on + strand at 806.170 kb on - strand at 806.250 kb on + strand at 806.266 kb on + strand at 806.266 kb on + strand at 806.289 kb on + strand at 806.324 kb on + strand, within MMP_RS04235 at 806.337 kb on + strand, within MMP_RS04235 at 806.369 kb on - strand, within MMP_RS04235 at 806.501 kb on - strand, within MMP_RS04235 at 806.502 kb on + strand, within MMP_RS04235 at 806.533 kb on + strand, within MMP_RS04235 at 806.651 kb on - strand, within MMP_RS04235 at 806.652 kb on + strand, within MMP_RS04235 at 806.667 kb on + strand, within MMP_RS04235 at 806.694 kb on - strand, within MMP_RS04235 at 806.702 kb on + strand, within MMP_RS04235 at 806.711 kb on + strand, within MMP_RS04235 at 806.761 kb on - strand, within MMP_RS04235 at 806.761 kb on - strand, within MMP_RS04235 at 806.789 kb on + strand, within MMP_RS04235 at 806.791 kb on - strand, within MMP_RS04235 at 806.792 kb on + strand, within MMP_RS04235 at 806.853 kb on - strand, within MMP_RS04235 at 806.856 kb on - strand, within MMP_RS04235 at 806.862 kb on - strand, within MMP_RS04235 at 806.862 kb on - strand, within MMP_RS04235 at 806.863 kb on + strand, within MMP_RS04235 at 806.863 kb on + strand, within MMP_RS04235 at 806.863 kb on + strand, within MMP_RS04235 at 806.863 kb on + strand, within MMP_RS04235 at 806.883 kb on - strand, within MMP_RS04235 at 806.887 kb on - strand, within MMP_RS04235 at 806.974 kb on - strand, within MMP_RS04235 at 807.007 kb on - strand, within MMP_RS04235 at 807.007 kb on - strand, within MMP_RS04235 at 807.010 kb on + strand, within MMP_RS04235 at 807.010 kb on + strand, within MMP_RS04235 at 807.015 kb on - strand, within MMP_RS04235 at 807.015 kb on - strand, within MMP_RS04235 at 807.015 kb on - strand, within MMP_RS04235 at 807.016 kb on + strand, within MMP_RS04235 at 807.016 kb on + strand, within MMP_RS04235 at 807.016 kb on + strand, within MMP_RS04235 at 807.016 kb on + strand, within MMP_RS04235 at 807.017 kb on - strand, within MMP_RS04235 at 807.017 kb on - strand, within MMP_RS04235 at 807.043 kb on - strand, within MMP_RS04235 at 807.043 kb on - strand, within MMP_RS04235 at 807.044 kb on + strand, within MMP_RS04235 at 807.044 kb on + strand, within MMP_RS04235 at 807.133 kb on - strand, within MMP_RS04235 at 807.194 kb on - strand at 807.194 kb on - strand at 807.194 kb on - strand at 807.194 kb on - strand at 807.194 kb on - strand at 807.195 kb on + strand at 807.195 kb on + strand at 807.195 kb on + strand at 807.196 kb on - strand at 807.196 kb on - strand at 807.197 kb on + strand at 807.197 kb on + strand at 807.197 kb on + strand at 807.197 kb on + strand at 807.197 kb on + strand at 807.198 kb on - strand at 807.198 kb on - strand at 807.199 kb on + strand at 807.199 kb on + strand at 807.227 kb on - strand at 807.227 kb on - strand at 807.268 kb on - strand at 807.287 kb on + strand at 807.287 kb on + strand at 807.308 kb on - strand at 807.558 kb on - strand, within MMP_RS04240 at 807.559 kb on + strand, within MMP_RS04240 at 807.677 kb on + strand, within MMP_RS04240 at 807.806 kb on + strand, within MMP_RS04240 at 807.806 kb on + strand, within MMP_RS04240 at 807.807 kb on - strand, within MMP_RS04240 at 807.807 kb on - strand, within MMP_RS04240 at 807.808 kb on + strand, within MMP_RS04240 at 807.808 kb on + strand, within MMP_RS04240 at 807.808 kb on + strand, within MMP_RS04240 at 807.810 kb on - strand, within MMP_RS04240 at 807.811 kb on + strand, within MMP_RS04240 at 807.839 kb on - strand, within MMP_RS04240 at 807.866 kb on - strand, within MMP_RS04240 at 807.918 kb on + strand, within MMP_RS04240 at 807.918 kb on + strand, within MMP_RS04240 at 807.939 kb on - strand, within MMP_RS04240 at 807.955 kb on - strand, within MMP_RS04240 at 807.956 kb on + strand, within MMP_RS04240 at 807.972 kb on - strand, within MMP_RS04240 at 807.973 kb on + strand, within MMP_RS04240 at 807.985 kb on + strand, within MMP_RS04240 at 807.985 kb on + strand, within MMP_RS04240 at 808.056 kb on - strand, within MMP_RS04240 at 808.057 kb on + strand, within MMP_RS04240 at 808.064 kb on + strand, within MMP_RS04240 at 808.101 kb on + strand, within MMP_RS04240 at 808.145 kb on - strand, within MMP_RS04240 at 808.146 kb on + strand, within MMP_RS04240 at 808.148 kb on + strand, within MMP_RS04240 at 808.152 kb on - strand, within MMP_RS04240 at 808.152 kb on - strand, within MMP_RS04240 at 808.152 kb on - strand, within MMP_RS04240 at 808.152 kb on - strand, within MMP_RS04240 at 808.152 kb on - strand, within MMP_RS04240 at 808.153 kb on + strand, within MMP_RS04240 at 808.153 kb on + strand, within MMP_RS04240 at 808.174 kb on - strand, within MMP_RS04240 at 808.181 kb on + strand, within MMP_RS04240 at 808.181 kb on + strand, within MMP_RS04240 at 808.238 kb on - strand, within MMP_RS04240 at 808.238 kb on - strand, within MMP_RS04240 at 808.263 kb on + strand, within MMP_RS04240 at 808.263 kb on + strand, within MMP_RS04240 at 808.267 kb on + strand, within MMP_RS04240 at 808.271 kb on + strand, within MMP_RS04240
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 805,200 + MMP_RS04230 0.70 -0.7 805,200 + MMP_RS04230 0.70 -1.2 805,234 + MMP_RS04230 0.71 -0.7 805,255 - MMP_RS04230 0.72 -0.1 805,305 + MMP_RS04230 0.74 -0.7 805,311 + MMP_RS04230 0.75 -0.1 805,316 + MMP_RS04230 0.75 -0.7 805,319 - MMP_RS04230 0.75 -0.2 805,320 + MMP_RS04230 0.75 +1.0 805,320 + MMP_RS04230 0.75 -0.4 805,320 + MMP_RS04230 0.75 +0.3 805,321 - MMP_RS04230 0.75 -0.9 805,321 - MMP_RS04230 0.75 +0.1 805,322 + MMP_RS04230 0.75 +0.0 805,322 + MMP_RS04230 0.75 +0.4 805,322 + MMP_RS04230 0.75 +0.3 805,352 - MMP_RS04230 0.77 -0.1 805,353 + MMP_RS04230 0.77 -0.9 805,353 + MMP_RS04230 0.77 -0.5 805,357 - MMP_RS04230 0.77 +0.4 805,358 + MMP_RS04230 0.77 +0.3 805,362 - MMP_RS04230 0.77 -0.7 805,362 - MMP_RS04230 0.77 +1.5 805,363 + MMP_RS04230 0.77 -0.1 805,363 + MMP_RS04230 0.77 +0.5 805,406 - MMP_RS04230 0.79 +0.8 805,452 - MMP_RS04230 0.81 +1.0 805,452 - MMP_RS04230 0.81 -0.6 805,452 - MMP_RS04230 0.81 +0.6 805,453 + MMP_RS04230 0.81 +1.1 805,454 - MMP_RS04230 0.81 +2.1 805,537 + MMP_RS04230 0.85 -0.5 805,541 + MMP_RS04230 0.85 -0.1 805,551 + MMP_RS04230 0.86 -1.0 805,551 + MMP_RS04230 0.86 -0.6 805,585 - MMP_RS04230 0.87 -0.6 805,644 - -0.2 805,645 + -0.1 805,648 - -0.6 805,706 + -0.6 805,761 - -0.4 805,761 - +0.3 805,761 - +0.2 805,761 - -0.9 805,762 + -0.2 805,776 + +0.5 805,777 - -0.4 805,777 - -0.9 805,811 - +0.2 805,873 + -0.1 805,887 + +0.1 805,891 - +0.6 805,892 + -1.4 805,892 + -0.2 805,892 + -0.8 805,895 - -0.5 805,895 - -0.5 805,895 - -0.2 805,895 - +0.6 805,895 - -1.2 805,895 - +1.2 805,895 - +0.3 805,895 - -1.1 805,896 + -0.6 805,896 + -0.3 805,897 - +1.2 805,909 + +1.5 805,916 + -0.3 805,916 + -0.6 805,922 - +1.0 805,927 - -0.7 805,928 + +0.1 805,966 - +0.1 805,967 + -0.6 805,970 - +0.0 805,971 + -0.7 805,978 + -0.5 806,007 - +0.0 806,007 - -0.5 806,016 + +0.1 806,028 + -0.4 806,050 - -0.3 806,050 - +0.5 806,055 + -0.0 806,114 - +0.0 806,115 + +3.4 806,115 + -0.9 806,120 - +0.1 806,138 - -0.9 806,139 + +0.2 806,142 - +0.5 806,155 - -0.4 806,158 - -0.4 806,159 + -0.3 806,166 + -1.3 806,167 - -1.2 806,168 + -0.8 806,170 - +1.2 806,250 + -0.9 806,266 + -0.0 806,266 + +0.0 806,289 + +1.5 806,324 + MMP_RS04235 0.12 -0.2 806,337 + MMP_RS04235 0.13 -2.9 806,369 - MMP_RS04235 0.16 -2.5 806,501 - MMP_RS04235 0.28 +0.2 806,502 + MMP_RS04235 0.28 +0.1 806,533 + MMP_RS04235 0.31 +0.6 806,651 - MMP_RS04235 0.42 +2.5 806,652 + MMP_RS04235 0.42 -2.4 806,667 + MMP_RS04235 0.43 +0.2 806,694 - MMP_RS04235 0.45 +1.0 806,702 + MMP_RS04235 0.46 +0.6 806,711 + MMP_RS04235 0.47 -0.1 806,761 - MMP_RS04235 0.51 +0.1 806,761 - MMP_RS04235 0.51 -0.2 806,789 + MMP_RS04235 0.54 -0.1 806,791 - MMP_RS04235 0.54 +0.1 806,792 + MMP_RS04235 0.54 +0.1 806,853 - MMP_RS04235 0.60 -1.0 806,856 - MMP_RS04235 0.60 -0.2 806,862 - MMP_RS04235 0.61 +0.1 806,862 - MMP_RS04235 0.61 -0.1 806,863 + MMP_RS04235 0.61 -0.6 806,863 + MMP_RS04235 0.61 -0.7 806,863 + MMP_RS04235 0.61 -0.6 806,863 + MMP_RS04235 0.61 +3.0 806,883 - MMP_RS04235 0.62 -1.3 806,887 - MMP_RS04235 0.63 -0.2 806,974 - MMP_RS04235 0.71 +0.1 807,007 - MMP_RS04235 0.74 +1.4 807,007 - MMP_RS04235 0.74 -0.3 807,010 + MMP_RS04235 0.74 -0.5 807,010 + MMP_RS04235 0.74 -1.5 807,015 - MMP_RS04235 0.74 -0.1 807,015 - MMP_RS04235 0.74 -1.6 807,015 - MMP_RS04235 0.74 -1.3 807,016 + MMP_RS04235 0.74 -0.7 807,016 + MMP_RS04235 0.74 -1.3 807,016 + MMP_RS04235 0.74 -1.3 807,016 + MMP_RS04235 0.74 -0.2 807,017 - MMP_RS04235 0.75 -0.3 807,017 - MMP_RS04235 0.75 +0.6 807,043 - MMP_RS04235 0.77 -1.0 807,043 - MMP_RS04235 0.77 -0.9 807,044 + MMP_RS04235 0.77 -1.4 807,044 + MMP_RS04235 0.77 -0.3 807,133 - MMP_RS04235 0.85 -0.1 807,194 - +0.4 807,194 - +0.1 807,194 - +0.1 807,194 - +1.2 807,194 - +0.3 807,195 + -0.5 807,195 + +1.5 807,195 + +1.1 807,196 - +0.3 807,196 - +0.6 807,197 + -0.8 807,197 + -0.5 807,197 + -0.2 807,197 + -0.8 807,197 + -0.5 807,198 - -0.9 807,198 - -1.2 807,199 + +0.4 807,199 + -0.1 807,227 - -1.6 807,227 - +0.7 807,268 - +1.0 807,287 + +0.2 807,287 + +0.1 807,308 - -0.8 807,558 - MMP_RS04240 0.10 +0.2 807,559 + MMP_RS04240 0.10 +0.7 807,677 + MMP_RS04240 0.20 +0.6 807,806 + MMP_RS04240 0.30 -0.1 807,806 + MMP_RS04240 0.30 +5.7 807,807 - MMP_RS04240 0.30 -0.9 807,807 - MMP_RS04240 0.30 +0.2 807,808 + MMP_RS04240 0.30 -0.2 807,808 + MMP_RS04240 0.30 +3.0 807,808 + MMP_RS04240 0.30 -1.2 807,810 - MMP_RS04240 0.31 +0.6 807,811 + MMP_RS04240 0.31 -0.2 807,839 - MMP_RS04240 0.33 -0.1 807,866 - MMP_RS04240 0.35 -1.0 807,918 + MMP_RS04240 0.39 -0.4 807,918 + MMP_RS04240 0.39 -0.7 807,939 - MMP_RS04240 0.41 -0.0 807,955 - MMP_RS04240 0.42 +0.4 807,956 + MMP_RS04240 0.42 +0.1 807,972 - MMP_RS04240 0.44 +1.2 807,973 + MMP_RS04240 0.44 -0.8 807,985 + MMP_RS04240 0.45 -0.5 807,985 + MMP_RS04240 0.45 -0.9 808,056 - MMP_RS04240 0.50 -0.6 808,057 + MMP_RS04240 0.50 -0.4 808,064 + MMP_RS04240 0.51 -0.4 808,101 + MMP_RS04240 0.54 -0.1 808,145 - MMP_RS04240 0.58 +0.6 808,146 + MMP_RS04240 0.58 -0.5 808,148 + MMP_RS04240 0.58 -1.6 808,152 - MMP_RS04240 0.58 +1.0 808,152 - MMP_RS04240 0.58 -0.4 808,152 - MMP_RS04240 0.58 +0.1 808,152 - MMP_RS04240 0.58 -0.5 808,152 - MMP_RS04240 0.58 -0.3 808,153 + MMP_RS04240 0.58 -0.8 808,153 + MMP_RS04240 0.58 +0.2 808,174 - MMP_RS04240 0.60 +1.3 808,181 + MMP_RS04240 0.60 +0.6 808,181 + MMP_RS04240 0.60 +0.8 808,238 - MMP_RS04240 0.65 +0.1 808,238 - MMP_RS04240 0.65 +0.0 808,263 + MMP_RS04240 0.67 -0.2 808,263 + MMP_RS04240 0.67 -0.4 808,267 + MMP_RS04240 0.67 -0.3 808,271 + MMP_RS04240 0.68 +0.2
Or see this region's nucleotide sequence