Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03705 and MMP_RS03710 are separated by 157 nucleotides MMP_RS03710 and MMP_RS03715 are separated by 216 nucleotides MMP_RS03715 and MMP_RS03720 are separated by 156 nucleotides
MMP_RS03705: MMP_RS03705 - (5-formylfuran-3-yl)methyl phosphate synthase, at 699,229 to 699,939
_RS03705
MMP_RS03710: MMP_RS03710 - tRNA-Arg, at 700,097 to 700,170
_RS03710
MMP_RS03715: MMP_RS03715 - class III signal peptide-containing protein, at 700,387 to 700,983
_RS03715
MMP_RS03720: MMP_RS03720 - cation-transporting P-type ATPase, at 701,140 to 703,920
_RS03720
Position (kb) 700
701 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 699.462 kb on + strand at 699.462 kb on + strand at 699.795 kb on - strand at 699.819 kb on + strand at 699.827 kb on - strand at 699.827 kb on - strand at 699.827 kb on - strand at 699.864 kb on - strand at 699.955 kb on + strand at 699.968 kb on + strand at 699.977 kb on + strand at 700.050 kb on - strand at 700.051 kb on + strand at 700.051 kb on + strand at 700.177 kb on - strand at 700.290 kb on - strand at 700.290 kb on - strand at 700.298 kb on + strand at 700.298 kb on + strand at 700.307 kb on + strand at 700.310 kb on + strand at 700.317 kb on + strand at 700.351 kb on - strand at 700.354 kb on + strand at 700.358 kb on - strand at 700.358 kb on - strand at 700.359 kb on + strand at 700.359 kb on + strand at 700.362 kb on - strand at 700.362 kb on - strand at 700.363 kb on + strand at 700.363 kb on + strand at 700.364 kb on - strand at 700.364 kb on - strand at 700.364 kb on - strand at 700.364 kb on - strand at 700.364 kb on - strand at 700.365 kb on + strand at 700.365 kb on + strand at 700.365 kb on + strand at 700.428 kb on + strand at 700.456 kb on - strand, within MMP_RS03715 at 700.648 kb on - strand, within MMP_RS03715 at 700.648 kb on - strand, within MMP_RS03715 at 700.648 kb on - strand, within MMP_RS03715 at 700.649 kb on + strand, within MMP_RS03715 at 700.649 kb on + strand, within MMP_RS03715 at 700.649 kb on + strand, within MMP_RS03715 at 700.649 kb on + strand, within MMP_RS03715 at 700.653 kb on - strand, within MMP_RS03715 at 700.654 kb on + strand, within MMP_RS03715 at 700.654 kb on + strand, within MMP_RS03715 at 700.656 kb on - strand, within MMP_RS03715 at 700.674 kb on + strand, within MMP_RS03715 at 700.698 kb on + strand, within MMP_RS03715 at 700.698 kb on + strand, within MMP_RS03715 at 700.698 kb on + strand, within MMP_RS03715 at 700.811 kb on + strand, within MMP_RS03715 at 700.838 kb on - strand, within MMP_RS03715 at 700.934 kb on + strand at 700.938 kb on + strand at 700.938 kb on + strand at 700.944 kb on + strand at 701.022 kb on + strand at 701.022 kb on + strand at 701.056 kb on + strand at 701.060 kb on - strand at 701.064 kb on - strand at 701.073 kb on - strand at 701.337 kb on + strand at 701.480 kb on - strand, within MMP_RS03720 at 701.553 kb on - strand, within MMP_RS03720 at 701.553 kb on - strand, within MMP_RS03720 at 701.554 kb on + strand, within MMP_RS03720 at 701.612 kb on + strand, within MMP_RS03720 at 701.618 kb on - strand, within MMP_RS03720 at 701.891 kb on - strand, within MMP_RS03720 at 701.896 kb on + strand, within MMP_RS03720 at 701.906 kb on - strand, within MMP_RS03720 at 701.906 kb on - strand, within MMP_RS03720 at 701.918 kb on - strand, within MMP_RS03720
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 699,462 + -1.0 699,462 + -1.1 699,795 - +1.2 699,819 + -1.7 699,827 - -0.3 699,827 - -0.2 699,827 - -1.0 699,864 - -0.0 699,955 + +1.0 699,968 + -0.4 699,977 + -1.8 700,050 - -1.6 700,051 + -1.0 700,051 + -0.0 700,177 - +0.3 700,290 - -0.4 700,290 - +0.2 700,298 + +1.6 700,298 + -0.3 700,307 + -0.3 700,310 + +0.1 700,317 + +0.2 700,351 - -0.2 700,354 + -0.0 700,358 - -1.4 700,358 - -0.1 700,359 + -1.5 700,359 + -0.7 700,362 - -1.4 700,362 - +0.1 700,363 + -0.6 700,363 + +1.8 700,364 - +1.0 700,364 - -0.3 700,364 - -0.1 700,364 - -1.4 700,364 - -0.5 700,365 + -0.3 700,365 + -0.5 700,365 + -0.8 700,428 + -0.1 700,456 - MMP_RS03715 0.12 +0.8 700,648 - MMP_RS03715 0.44 +0.3 700,648 - MMP_RS03715 0.44 -0.2 700,648 - MMP_RS03715 0.44 -0.1 700,649 + MMP_RS03715 0.44 +0.9 700,649 + MMP_RS03715 0.44 -0.9 700,649 + MMP_RS03715 0.44 +0.0 700,649 + MMP_RS03715 0.44 -0.1 700,653 - MMP_RS03715 0.45 +3.7 700,654 + MMP_RS03715 0.45 +0.2 700,654 + MMP_RS03715 0.45 -1.0 700,656 - MMP_RS03715 0.45 +0.5 700,674 + MMP_RS03715 0.48 -0.8 700,698 + MMP_RS03715 0.52 +0.6 700,698 + MMP_RS03715 0.52 +0.9 700,698 + MMP_RS03715 0.52 +0.5 700,811 + MMP_RS03715 0.71 -0.4 700,838 - MMP_RS03715 0.76 -1.0 700,934 + +0.4 700,938 + -1.1 700,938 + -0.8 700,944 + +0.2 701,022 + -0.9 701,022 + +0.5 701,056 + +1.2 701,060 - -0.0 701,064 - -0.4 701,073 - +0.2 701,337 + -0.3 701,480 - MMP_RS03720 0.12 -0.4 701,553 - MMP_RS03720 0.15 -0.3 701,553 - MMP_RS03720 0.15 -1.6 701,554 + MMP_RS03720 0.15 -0.3 701,612 + MMP_RS03720 0.17 +0.2 701,618 - MMP_RS03720 0.17 -0.5 701,891 - MMP_RS03720 0.27 -0.6 701,896 + MMP_RS03720 0.27 -0.3 701,906 - MMP_RS03720 0.28 -0.6 701,906 - MMP_RS03720 0.28 +2.8 701,918 - MMP_RS03720 0.28 +0.8
Or see this region's nucleotide sequence