Experiment: H2 McCas- cells grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03695 and MMP_RS03700 are separated by 12 nucleotides MMP_RS03700 and MMP_RS03705 are separated by 204 nucleotides
MMP_RS03695: MMP_RS03695 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 696,475 to 697,758
_RS03695
MMP_RS03700: MMP_RS03700 - sodium:proton antiporter, at 697,771 to 699,024
_RS03700
MMP_RS03705: MMP_RS03705 - (5-formylfuran-3-yl)methyl phosphate synthase, at 699,229 to 699,939
_RS03705
Position (kb) 697
698
699
700 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8
9
10
11 at 696.787 kb on + strand at 696.857 kb on + strand at 696.884 kb on - strand at 696.973 kb on - strand at 696.974 kb on + strand at 697.175 kb on + strand at 697.317 kb on + strand at 697.372 kb on + strand at 697.382 kb on + strand at 697.402 kb on - strand at 697.556 kb on + strand at 697.614 kb on - strand at 697.889 kb on - strand at 697.932 kb on - strand, within MMP_RS03700 at 697.940 kb on - strand, within MMP_RS03700 at 697.941 kb on + strand, within MMP_RS03700 at 698.235 kb on - strand, within MMP_RS03700 at 698.256 kb on + strand, within MMP_RS03700 at 698.256 kb on + strand, within MMP_RS03700 at 698.257 kb on - strand, within MMP_RS03700 at 698.363 kb on + strand, within MMP_RS03700 at 698.363 kb on + strand, within MMP_RS03700 at 698.551 kb on - strand, within MMP_RS03700 at 698.616 kb on - strand, within MMP_RS03700 at 698.617 kb on + strand, within MMP_RS03700 at 698.617 kb on + strand, within MMP_RS03700 at 698.617 kb on + strand, within MMP_RS03700 at 698.654 kb on - strand, within MMP_RS03700 at 698.658 kb on - strand, within MMP_RS03700 at 698.676 kb on - strand, within MMP_RS03700 at 698.676 kb on - strand, within MMP_RS03700 at 698.677 kb on + strand, within MMP_RS03700 at 698.690 kb on - strand, within MMP_RS03700 at 698.690 kb on - strand, within MMP_RS03700 at 698.691 kb on + strand, within MMP_RS03700 at 698.691 kb on + strand, within MMP_RS03700 at 698.691 kb on + strand, within MMP_RS03700 at 698.691 kb on + strand, within MMP_RS03700 at 698.692 kb on - strand, within MMP_RS03700 at 698.693 kb on + strand, within MMP_RS03700 at 698.693 kb on + strand, within MMP_RS03700 at 698.694 kb on - strand, within MMP_RS03700 at 698.768 kb on + strand, within MMP_RS03700 at 698.903 kb on + strand at 698.913 kb on - strand at 698.945 kb on + strand at 698.945 kb on + strand at 698.965 kb on - strand at 698.999 kb on - strand at 699.038 kb on + strand at 699.041 kb on - strand at 699.047 kb on + strand at 699.048 kb on - strand at 699.048 kb on - strand at 699.049 kb on + strand at 699.075 kb on - strand at 699.106 kb on - strand at 699.106 kb on - strand at 699.113 kb on - strand at 699.134 kb on + strand at 699.171 kb on - strand at 699.176 kb on + strand at 699.176 kb on + strand at 699.182 kb on + strand at 699.186 kb on + strand at 699.186 kb on + strand at 699.197 kb on - strand at 699.298 kb on + strand at 699.350 kb on - strand at 699.462 kb on + strand at 699.462 kb on + strand at 699.795 kb on - strand at 699.819 kb on + strand at 699.827 kb on - strand at 699.827 kb on - strand at 699.827 kb on - strand at 699.864 kb on - strand at 699.955 kb on + strand at 699.968 kb on + strand at 699.977 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells grown in liquid medium with neomycin remove 696,787 + +0.2 696,857 + +1.6 696,884 - -0.3 696,973 - -0.4 696,974 + +1.0 697,175 + +2.6 697,317 + -0.8 697,372 + +1.0 697,382 + +2.6 697,402 - -1.0 697,556 + +0.2 697,614 - +0.2 697,889 - -0.8 697,932 - MMP_RS03700 0.13 +0.6 697,940 - MMP_RS03700 0.13 -0.6 697,941 + MMP_RS03700 0.14 +1.6 698,235 - MMP_RS03700 0.37 -0.0 698,256 + MMP_RS03700 0.39 +1.3 698,256 + MMP_RS03700 0.39 -0.0 698,257 - MMP_RS03700 0.39 +3.3 698,363 + MMP_RS03700 0.47 +3.0 698,363 + MMP_RS03700 0.47 -1.4 698,551 - MMP_RS03700 0.62 +6.2 698,616 - MMP_RS03700 0.67 +5.7 698,617 + MMP_RS03700 0.67 +10.8 698,617 + MMP_RS03700 0.67 +11.0 698,617 + MMP_RS03700 0.67 +8.9 698,654 - MMP_RS03700 0.70 +6.8 698,658 - MMP_RS03700 0.71 +5.4 698,676 - MMP_RS03700 0.72 +5.9 698,676 - MMP_RS03700 0.72 +5.2 698,677 + MMP_RS03700 0.72 +7.2 698,690 - MMP_RS03700 0.73 +5.6 698,690 - MMP_RS03700 0.73 +5.0 698,691 + MMP_RS03700 0.73 +7.3 698,691 + MMP_RS03700 0.73 +7.4 698,691 + MMP_RS03700 0.73 +6.7 698,691 + MMP_RS03700 0.73 +6.5 698,692 - MMP_RS03700 0.73 +4.8 698,693 + MMP_RS03700 0.74 +7.2 698,693 + MMP_RS03700 0.74 +7.0 698,694 - MMP_RS03700 0.74 +5.8 698,768 + MMP_RS03700 0.80 +7.6 698,903 + +8.9 698,913 - +5.2 698,945 + +8.7 698,945 + +10.4 698,965 - +4.4 698,999 - +7.0 699,038 + +7.3 699,041 - -0.6 699,047 + +8.4 699,048 - +0.6 699,048 - +3.0 699,049 + +8.1 699,075 - +1.4 699,106 - +1.5 699,106 - +1.7 699,113 - +1.6 699,134 + +4.5 699,171 - +0.7 699,176 + -1.3 699,176 + -0.3 699,182 + -0.4 699,186 + +1.6 699,186 + -0.4 699,197 - +1.4 699,298 + +2.6 699,350 - -0.3 699,462 + -1.0 699,462 + -1.1 699,795 - +1.2 699,819 + -1.7 699,827 - -0.3 699,827 - -0.2 699,827 - -1.0 699,864 - -0.0 699,955 + +1.0 699,968 + -0.4 699,977 + -1.8
Or see this region's nucleotide sequence