Experiment: 1-ethyl-3-methylimidazolium acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_14930 and AO356_14935 are separated by 46 nucleotides AO356_14935 and AO356_14940 are separated by 16 nucleotides AO356_14940 and AO356_14945 are separated by 248 nucleotides AO356_14945 and AO356_14950 overlap by 4 nucleotides
AO356_14930: AO356_14930 - diaminohydroxyphosphoribosylaminopyrimidine deaminase, at 3,378,161 to 3,379,294
_14930
AO356_14935: AO356_14935 - riboflavin synthase subunit alpha, at 3,379,341 to 3,380,018
_14935
AO356_14940: AO356_14940 - 3,4-dihydroxy-2-butanone 4-phosphate synthase, at 3,380,035 to 3,381,126
_14940
AO356_14945: AO356_14945 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,381,375 to 3,381,851
_14945
AO356_14950: AO356_14950 - antitermination protein NusB, at 3,381,848 to 3,382,348
_14950
Position (kb)
3380
3381
3382 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3379.290 kb on + strand at 3379.290 kb on + strand at 3379.291 kb on - strand at 3379.291 kb on - strand at 3379.291 kb on - strand at 3379.291 kb on - strand at 3379.292 kb on + strand at 3379.292 kb on + strand at 3379.292 kb on + strand at 3379.292 kb on + strand at 3379.923 kb on - strand, within AO356_14935 at 3379.923 kb on - strand, within AO356_14935 at 3380.046 kb on + strand at 3380.046 kb on + strand at 3380.046 kb on + strand at 3380.047 kb on - strand at 3380.047 kb on - strand at 3380.047 kb on - strand at 3380.047 kb on - strand at 3380.357 kb on + strand, within AO356_14940 at 3380.358 kb on - strand, within AO356_14940 at 3380.646 kb on + strand, within AO356_14940 at 3380.646 kb on + strand, within AO356_14940 at 3380.647 kb on - strand, within AO356_14940 at 3380.647 kb on - strand, within AO356_14940 at 3380.647 kb on - strand, within AO356_14940 at 3380.647 kb on - strand, within AO356_14940 at 3380.757 kb on + strand, within AO356_14940 at 3380.762 kb on + strand, within AO356_14940 at 3380.762 kb on + strand, within AO356_14940 at 3380.762 kb on + strand, within AO356_14940 at 3380.762 kb on + strand, within AO356_14940 at 3380.762 kb on + strand, within AO356_14940 at 3380.762 kb on + strand, within AO356_14940 at 3380.762 kb on + strand, within AO356_14940 at 3380.763 kb on - strand, within AO356_14940 at 3380.763 kb on - strand, within AO356_14940 at 3380.763 kb on - strand, within AO356_14940 at 3380.989 kb on + strand, within AO356_14940 at 3380.989 kb on + strand, within AO356_14940 at 3380.989 kb on + strand, within AO356_14940 at 3380.989 kb on + strand, within AO356_14940 at 3381.072 kb on + strand at 3381.104 kb on + strand at 3381.104 kb on + strand at 3381.109 kb on + strand at 3381.109 kb on + strand at 3381.109 kb on + strand at 3381.124 kb on + strand at 3381.158 kb on + strand at 3381.158 kb on + strand at 3381.158 kb on + strand at 3381.158 kb on + strand at 3381.164 kb on + strand at 3381.168 kb on + strand at 3381.168 kb on + strand at 3381.168 kb on + strand at 3381.168 kb on + strand at 3381.168 kb on + strand at 3381.168 kb on + strand at 3381.235 kb on + strand at 3381.317 kb on + strand at 3381.317 kb on + strand at 3381.317 kb on + strand at 3381.320 kb on + strand at 3381.340 kb on + strand at 3381.340 kb on + strand at 3381.340 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 10 mM remove 3,379,290 + +0.5 3,379,290 + +1.1 3,379,291 - +0.5 3,379,291 - +0.0 3,379,291 - +0.2 3,379,291 - -0.1 3,379,292 + +0.3 3,379,292 + -0.1 3,379,292 + -0.7 3,379,292 + -0.2 3,379,923 - AO356_14935 0.86 -0.9 3,379,923 - AO356_14935 0.86 -1.5 3,380,046 + -2.5 3,380,046 + -1.6 3,380,046 + -0.0 3,380,047 - -2.3 3,380,047 - -0.8 3,380,047 - -2.1 3,380,047 - -0.5 3,380,357 + AO356_14940 0.29 -1.2 3,380,358 - AO356_14940 0.30 -0.3 3,380,646 + AO356_14940 0.56 -0.3 3,380,646 + AO356_14940 0.56 -0.6 3,380,647 - AO356_14940 0.56 -2.8 3,380,647 - AO356_14940 0.56 -1.0 3,380,647 - AO356_14940 0.56 -0.5 3,380,647 - AO356_14940 0.56 -1.3 3,380,757 + AO356_14940 0.66 +0.1 3,380,762 + AO356_14940 0.67 -2.3 3,380,762 + AO356_14940 0.67 -1.4 3,380,762 + AO356_14940 0.67 -2.1 3,380,762 + AO356_14940 0.67 -1.8 3,380,762 + AO356_14940 0.67 -1.7 3,380,762 + AO356_14940 0.67 -0.4 3,380,762 + AO356_14940 0.67 -0.7 3,380,763 - AO356_14940 0.67 +1.0 3,380,763 - AO356_14940 0.67 -0.3 3,380,763 - AO356_14940 0.67 -0.5 3,380,989 + AO356_14940 0.87 -1.3 3,380,989 + AO356_14940 0.87 -0.9 3,380,989 + AO356_14940 0.87 -1.0 3,380,989 + AO356_14940 0.87 -0.8 3,381,072 + -1.5 3,381,104 + +1.2 3,381,104 + +1.4 3,381,109 + +0.5 3,381,109 + -0.3 3,381,109 + -0.1 3,381,124 + -0.5 3,381,158 + +0.1 3,381,158 + -0.5 3,381,158 + -1.9 3,381,158 + +1.3 3,381,164 + -1.0 3,381,168 + -0.4 3,381,168 + +0.6 3,381,168 + +0.8 3,381,168 + -0.6 3,381,168 + +0.5 3,381,168 + +0.2 3,381,235 + -0.5 3,381,317 + -0.1 3,381,317 + -0.3 3,381,317 + +2.0 3,381,320 + +0.2 3,381,340 + -1.5 3,381,340 + +0.1 3,381,340 + -0.4
Or see this region's nucleotide sequence