Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2607 and Shew_2608 are separated by 123 nucleotides Shew_2608 and Shew_2609 are separated by 60 nucleotides Shew_2609 and Shew_2610 overlap by 4 nucleotides
Shew_2607: Shew_2607 - putative branched-chain amino acid ABC transporter (RefSeq), at 3,073,024 to 3,074,268
_2607
Shew_2608: Shew_2608 - inner-membrane translocator (RefSeq), at 3,074,392 to 3,075,465
_2608
Shew_2609: Shew_2609 - inner-membrane translocator (RefSeq), at 3,075,526 to 3,076,419
_2609
Shew_2610: Shew_2610 - AMP-dependent synthetase and ligase (RefSeq), at 3,076,416 to 3,078,383
_2610
Position (kb)
3074
3075
3076 Strain fitness (log2 ratio)
-1
0
1 at 3073.733 kb on + strand, within Shew_2607 at 3073.754 kb on + strand, within Shew_2607 at 3073.853 kb on - strand, within Shew_2607 at 3074.176 kb on - strand at 3074.243 kb on + strand at 3074.383 kb on + strand at 3074.413 kb on - strand at 3074.413 kb on - strand at 3074.423 kb on + strand at 3074.518 kb on - strand, within Shew_2608 at 3074.545 kb on + strand, within Shew_2608 at 3074.718 kb on - strand, within Shew_2608 at 3075.007 kb on - strand, within Shew_2608 at 3075.344 kb on + strand, within Shew_2608 at 3075.421 kb on - strand at 3075.524 kb on + strand at 3075.611 kb on + strand at 3075.763 kb on + strand, within Shew_2609 at 3076.118 kb on - strand, within Shew_2609 at 3076.343 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,073,733 + Shew_2607 0.57 -0.0 3,073,754 + Shew_2607 0.59 +0.2 3,073,853 - Shew_2607 0.67 +0.1 3,074,176 - +0.1 3,074,243 + +0.1 3,074,383 + +0.2 3,074,413 - +0.0 3,074,413 - +0.0 3,074,423 + +0.6 3,074,518 - Shew_2608 0.12 -0.1 3,074,545 + Shew_2608 0.14 -0.8 3,074,718 - Shew_2608 0.30 -0.8 3,075,007 - Shew_2608 0.57 +0.0 3,075,344 + Shew_2608 0.89 -0.1 3,075,421 - -0.5 3,075,524 + +0.0 3,075,611 + -0.0 3,075,763 + Shew_2609 0.27 -0.1 3,076,118 - Shew_2609 0.66 +0.3 3,076,343 + +0.2
Or see this region's nucleotide sequence