Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1500 and Shew_1501 are separated by 84 nucleotides Shew_1501 and Shew_1502 are separated by 212 nucleotides Shew_1502 and Shew_1503 are separated by 170 nucleotides
Shew_1500: Shew_1500 - signal transduction protein (RefSeq), at 1,721,417 to 1,721,821
_1500
Shew_1501: Shew_1501 - glutaredoxin (RefSeq), at 1,721,906 to 1,722,262
_1501
Shew_1502: Shew_1502 - hypothetical protein (RefSeq), at 1,722,475 to 1,725,834
_1502
Shew_1503: Shew_1503 - oxidoreductase FAD-binding subunit (RefSeq), at 1,726,005 to 1,726,997
_1503
Position (kb)
1722
1723
1724
1725
1726 Strain fitness (log2 ratio)
-1
0
1 at 1721.587 kb on - strand, within Shew_1500 at 1721.721 kb on - strand, within Shew_1500 at 1722.563 kb on + strand at 1723.016 kb on - strand, within Shew_1502 at 1723.016 kb on - strand, within Shew_1502 at 1723.245 kb on + strand, within Shew_1502 at 1723.253 kb on - strand, within Shew_1502 at 1723.655 kb on - strand, within Shew_1502 at 1723.655 kb on - strand, within Shew_1502 at 1724.257 kb on - strand, within Shew_1502 at 1724.295 kb on - strand, within Shew_1502 at 1724.594 kb on + strand, within Shew_1502 at 1725.425 kb on - strand, within Shew_1502 at 1725.501 kb on - strand at 1725.892 kb on + strand at 1726.012 kb on + strand at 1726.067 kb on - strand at 1726.067 kb on - strand at 1726.183 kb on + strand, within Shew_1503
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,721,587 - Shew_1500 0.42 +0.6 1,721,721 - Shew_1500 0.75 +0.6 1,722,563 + -0.3 1,723,016 - Shew_1502 0.16 -0.2 1,723,016 - Shew_1502 0.16 -0.5 1,723,245 + Shew_1502 0.23 -0.5 1,723,253 - Shew_1502 0.23 -0.1 1,723,655 - Shew_1502 0.35 -0.4 1,723,655 - Shew_1502 0.35 -0.4 1,724,257 - Shew_1502 0.53 -0.4 1,724,295 - Shew_1502 0.54 -0.7 1,724,594 + Shew_1502 0.63 +0.0 1,725,425 - Shew_1502 0.88 +0.1 1,725,501 - -0.4 1,725,892 + -0.1 1,726,012 + +0.4 1,726,067 - +0.1 1,726,067 - +0.2 1,726,183 + Shew_1503 0.18 -0.0
Or see this region's nucleotide sequence