Experiment: nitrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3317 and Dshi_3318 are separated by 156 nucleotides Dshi_3318 and Dshi_3319 are separated by 54 nucleotides Dshi_3319 and Dshi_3320 are separated by 13 nucleotides
Dshi_3317: Dshi_3317 - phosphoserine aminotransferase (RefSeq), at 3,479,701 to 3,480,855
_3317
Dshi_3318: Dshi_3318 - D-3-phosphoglycerate dehydrogenase (RefSeq), at 3,481,012 to 3,482,607
_3318
Dshi_3319: Dshi_3319 - metallophosphoesterase (RefSeq), at 3,482,662 to 3,483,396
_3319
Dshi_3320: Dshi_3320 - hypothetical protein (RefSeq), at 3,483,410 to 3,483,616
_3320
Position (kb) 3481
3482
3483 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 3480.955 kb on - strand at 3482.110 kb on + strand, within Dshi_3318 at 3482.508 kb on + strand at 3482.566 kb on - strand at 3483.120 kb on - strand, within Dshi_3319 at 3483.165 kb on + strand, within Dshi_3319 at 3483.175 kb on - strand, within Dshi_3319 at 3483.250 kb on - strand, within Dshi_3319 at 3483.259 kb on + strand, within Dshi_3319 at 3483.341 kb on - strand at 3483.341 kb on - strand at 3483.416 kb on - strand at 3483.555 kb on - strand, within Dshi_3320
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate (N) remove 3,480,955 - -2.6 3,482,110 + Dshi_3318 0.69 -4.1 3,482,508 + -0.3 3,482,566 - -0.5 3,483,120 - Dshi_3319 0.62 +0.0 3,483,165 + Dshi_3319 0.68 +0.7 3,483,175 - Dshi_3319 0.70 +0.1 3,483,250 - Dshi_3319 0.80 +0.1 3,483,259 + Dshi_3319 0.81 +0.6 3,483,341 - -0.1 3,483,341 - +0.3 3,483,416 - -0.2 3,483,555 - Dshi_3320 0.70 -1.2
Or see this region's nucleotide sequence