Experiment: nitrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0998 and Dshi_0999 are separated by 52 nucleotides Dshi_0999 and Dshi_1000 are separated by 3 nucleotides Dshi_1000 and Dshi_1001 are separated by 91 nucleotides
Dshi_0998: Dshi_0998 - adenine deaminase (RefSeq), at 1,030,013 to 1,031,707
_0998
Dshi_0999: Dshi_0999 - Bile acid:sodium symporter (RefSeq), at 1,031,760 to 1,032,641
_0999
Dshi_1000: Dshi_1000 - AMP-dependent synthetase and ligase (RefSeq), at 1,032,645 to 1,034,366
_1000
Dshi_1001: Dshi_1001 - D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding (RefSeq), at 1,034,458 to 1,035,408
_1001
Position (kb)
1032
1033
1034
1035 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1031.648 kb on + strand at 1031.675 kb on + strand at 1031.678 kb on - strand at 1031.758 kb on + strand at 1031.867 kb on + strand, within Dshi_0999 at 1031.917 kb on - strand, within Dshi_0999 at 1031.947 kb on + strand, within Dshi_0999 at 1031.965 kb on - strand, within Dshi_0999 at 1031.965 kb on - strand, within Dshi_0999 at 1032.032 kb on + strand, within Dshi_0999 at 1032.040 kb on - strand, within Dshi_0999 at 1032.078 kb on - strand, within Dshi_0999 at 1032.098 kb on + strand, within Dshi_0999 at 1032.186 kb on - strand, within Dshi_0999 at 1032.194 kb on + strand, within Dshi_0999 at 1032.273 kb on - strand, within Dshi_0999 at 1032.321 kb on - strand, within Dshi_0999 at 1032.323 kb on + strand, within Dshi_0999 at 1032.366 kb on - strand, within Dshi_0999 at 1032.386 kb on + strand, within Dshi_0999 at 1032.415 kb on - strand, within Dshi_0999 at 1032.460 kb on - strand, within Dshi_0999 at 1032.534 kb on - strand, within Dshi_0999 at 1032.639 kb on - strand at 1032.648 kb on + strand at 1032.656 kb on - strand at 1032.753 kb on + strand at 1032.771 kb on - strand at 1032.839 kb on - strand, within Dshi_1000 at 1032.867 kb on + strand, within Dshi_1000 at 1032.894 kb on + strand, within Dshi_1000 at 1032.962 kb on - strand, within Dshi_1000 at 1032.963 kb on - strand, within Dshi_1000 at 1032.965 kb on - strand, within Dshi_1000 at 1033.212 kb on - strand, within Dshi_1000 at 1033.251 kb on + strand, within Dshi_1000 at 1033.252 kb on + strand, within Dshi_1000 at 1033.401 kb on + strand, within Dshi_1000 at 1033.426 kb on + strand, within Dshi_1000 at 1033.447 kb on + strand, within Dshi_1000 at 1033.649 kb on - strand, within Dshi_1000 at 1033.694 kb on - strand, within Dshi_1000 at 1033.985 kb on - strand, within Dshi_1000 at 1034.014 kb on + strand, within Dshi_1000 at 1034.032 kb on + strand, within Dshi_1000 at 1034.053 kb on + strand, within Dshi_1000 at 1034.085 kb on + strand, within Dshi_1000 at 1034.156 kb on - strand, within Dshi_1000 at 1034.269 kb on - strand at 1034.406 kb on + strand at 1034.435 kb on + strand at 1034.435 kb on + strand at 1034.440 kb on + strand at 1034.443 kb on - strand at 1034.480 kb on - strand at 1034.593 kb on - strand, within Dshi_1001 at 1034.635 kb on + strand, within Dshi_1001 at 1034.672 kb on + strand, within Dshi_1001 at 1034.730 kb on + strand, within Dshi_1001 at 1034.737 kb on - strand, within Dshi_1001 at 1034.803 kb on + strand, within Dshi_1001 at 1034.866 kb on - strand, within Dshi_1001 at 1034.866 kb on - strand, within Dshi_1001 at 1034.926 kb on - strand, within Dshi_1001 at 1035.042 kb on + strand, within Dshi_1001 at 1035.075 kb on + strand, within Dshi_1001 at 1035.175 kb on + strand, within Dshi_1001 at 1035.188 kb on - strand, within Dshi_1001
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate (N) remove 1,031,648 + -0.2 1,031,675 + -0.3 1,031,678 - -0.6 1,031,758 + -0.9 1,031,867 + Dshi_0999 0.12 -0.1 1,031,917 - Dshi_0999 0.18 -1.5 1,031,947 + Dshi_0999 0.21 -0.1 1,031,965 - Dshi_0999 0.23 -1.8 1,031,965 - Dshi_0999 0.23 -0.9 1,032,032 + Dshi_0999 0.31 -0.5 1,032,040 - Dshi_0999 0.32 -0.3 1,032,078 - Dshi_0999 0.36 -0.3 1,032,098 + Dshi_0999 0.38 +1.4 1,032,186 - Dshi_0999 0.48 -0.3 1,032,194 + Dshi_0999 0.49 +0.5 1,032,273 - Dshi_0999 0.58 +0.2 1,032,321 - Dshi_0999 0.64 -0.3 1,032,323 + Dshi_0999 0.64 -0.5 1,032,366 - Dshi_0999 0.69 -0.7 1,032,386 + Dshi_0999 0.71 +0.0 1,032,415 - Dshi_0999 0.74 -0.4 1,032,460 - Dshi_0999 0.79 +0.4 1,032,534 - Dshi_0999 0.88 +0.5 1,032,639 - +1.6 1,032,648 + +1.7 1,032,656 - -0.3 1,032,753 + +0.1 1,032,771 - +0.4 1,032,839 - Dshi_1000 0.11 -3.3 1,032,867 + Dshi_1000 0.13 +0.6 1,032,894 + Dshi_1000 0.14 -1.5 1,032,962 - Dshi_1000 0.18 -0.1 1,032,963 - Dshi_1000 0.18 -0.7 1,032,965 - Dshi_1000 0.19 -1.7 1,033,212 - Dshi_1000 0.33 +0.6 1,033,251 + Dshi_1000 0.35 -1.6 1,033,252 + Dshi_1000 0.35 +0.6 1,033,401 + Dshi_1000 0.44 -0.5 1,033,426 + Dshi_1000 0.45 +0.7 1,033,447 + Dshi_1000 0.47 -0.8 1,033,649 - Dshi_1000 0.58 -0.7 1,033,694 - Dshi_1000 0.61 -1.5 1,033,985 - Dshi_1000 0.78 -1.1 1,034,014 + Dshi_1000 0.80 -0.4 1,034,032 + Dshi_1000 0.81 -1.1 1,034,053 + Dshi_1000 0.82 -0.4 1,034,085 + Dshi_1000 0.84 -0.5 1,034,156 - Dshi_1000 0.88 -0.8 1,034,269 - +0.4 1,034,406 + -0.0 1,034,435 + -0.3 1,034,435 + -0.5 1,034,440 + -0.4 1,034,443 - -1.1 1,034,480 - -0.1 1,034,593 - Dshi_1001 0.14 -0.1 1,034,635 + Dshi_1001 0.19 +0.1 1,034,672 + Dshi_1001 0.23 +1.3 1,034,730 + Dshi_1001 0.29 +0.5 1,034,737 - Dshi_1001 0.29 +0.5 1,034,803 + Dshi_1001 0.36 -0.6 1,034,866 - Dshi_1001 0.43 -0.5 1,034,866 - Dshi_1001 0.43 +0.5 1,034,926 - Dshi_1001 0.49 +0.3 1,035,042 + Dshi_1001 0.61 -0.3 1,035,075 + Dshi_1001 0.65 -0.7 1,035,175 + Dshi_1001 0.75 -2.0 1,035,188 - Dshi_1001 0.77 +1.8
Or see this region's nucleotide sequence