Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_25225

Experiment: 1-ethyl-3-methylimidazolium acetate 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_25215 and AO356_25220 overlap by 8 nucleotidesAO356_25220 and AO356_25225 are separated by 10 nucleotidesAO356_25225 and AO356_25230 are separated by 25 nucleotides AO356_25215: AO356_25215 - nitrate reductase, at 5,708,501 to 5,709,256 _25215 AO356_25220: AO356_25220 - nitrate reductase, at 5,709,249 to 5,709,929 _25220 AO356_25225: AO356_25225 - peptidylprolyl isomerase, at 5,709,940 to 5,710,893 _25225 AO356_25230: AO356_25230 - cyclic pyranopterin phosphate synthase MoaA, at 5,710,919 to 5,711,911 _25230 Position (kb) 5709 5710 5711Strain fitness (log2 ratio) -1 0 1at 5708.960 kb on + strand, within AO356_25215at 5709.282 kb on + strandat 5709.282 kb on + strandat 5709.288 kb on + strandat 5709.288 kb on + strandat 5709.289 kb on - strandat 5709.345 kb on + strand, within AO356_25220at 5709.345 kb on + strand, within AO356_25220at 5709.345 kb on + strand, within AO356_25220at 5709.478 kb on - strand, within AO356_25220at 5709.478 kb on - strand, within AO356_25220at 5710.694 kb on - strand, within AO356_25225at 5710.816 kb on + strandat 5710.817 kb on - strandat 5711.007 kb on - strandat 5711.007 kb on - strandat 5711.160 kb on + strand, within AO356_25230at 5711.161 kb on - strand, within AO356_25230at 5711.450 kb on + strand, within AO356_25230at 5711.450 kb on + strand, within AO356_25230at 5711.618 kb on + strand, within AO356_25230at 5711.619 kb on - strand, within AO356_25230at 5711.677 kb on + strand, within AO356_25230

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 5 mM
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5,708,960 + AO356_25215 0.61 -0.3
5,709,282 + +1.0
5,709,282 + +0.7
5,709,288 + -0.4
5,709,288 + -0.3
5,709,289 - -0.6
5,709,345 + AO356_25220 0.14 -1.4
5,709,345 + AO356_25220 0.14 +0.8
5,709,345 + AO356_25220 0.14 -0.0
5,709,478 - AO356_25220 0.34 -0.8
5,709,478 - AO356_25220 0.34 -0.2
5,710,694 - AO356_25225 0.79 +1.0
5,710,816 + +0.4
5,710,817 - -0.2
5,711,007 - -1.7
5,711,007 - -0.0
5,711,160 + AO356_25230 0.24 -0.4
5,711,161 - AO356_25230 0.24 +0.5
5,711,450 + AO356_25230 0.53 +0.6
5,711,450 + AO356_25230 0.53 +1.1
5,711,618 + AO356_25230 0.70 -0.4
5,711,619 - AO356_25230 0.70 +0.2
5,711,677 + AO356_25230 0.76 -0.1

Or see this region's nucleotide sequence