Experiment: 1-ethyl-3-methylimidazolium acetate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22100 and AO356_22105 are separated by 169 nucleotides AO356_22105 and AO356_22110 overlap by 4 nucleotides AO356_22110 and AO356_22115 are separated by 59 nucleotides AO356_22115 and AO356_22120 are separated by 176 nucleotides
AO356_22100: AO356_22100 - adenylosuccinate lyase, at 4,947,248 to 4,948,618
_22100
AO356_22105: AO356_22105 - DNA repair protein, at 4,948,788 to 4,949,411
_22105
AO356_22110: AO356_22110 - tRNA 2-thiouridylase, at 4,949,408 to 4,950,532
_22110
AO356_22115: AO356_22115 - NUDIX hydrolase, at 4,950,592 to 4,951,038
_22115
AO356_22120: AO356_22120 - hypothetical protein, at 4,951,215 to 4,952,096
_22120
Position (kb)
4949
4950
4951 Strain fitness (log2 ratio)
-1
0
1
2 at 4948.664 kb on - strand at 4948.664 kb on - strand at 4948.707 kb on + strand at 4948.707 kb on + strand at 4948.707 kb on + strand at 4948.713 kb on + strand at 4948.714 kb on - strand at 4948.721 kb on + strand at 4948.722 kb on - strand at 4948.722 kb on - strand at 4948.722 kb on - strand at 4948.732 kb on - strand at 4948.732 kb on - strand at 4948.732 kb on - strand at 4948.734 kb on + strand at 4948.735 kb on - strand at 4948.735 kb on - strand at 4948.735 kb on - strand at 4948.735 kb on - strand at 4948.735 kb on - strand at 4948.735 kb on - strand at 4948.789 kb on + strand at 4948.789 kb on + strand at 4948.789 kb on + strand at 4948.790 kb on - strand at 4949.156 kb on - strand, within AO356_22105 at 4950.641 kb on + strand, within AO356_22115 at 4950.641 kb on + strand, within AO356_22115 at 4950.642 kb on - strand, within AO356_22115 at 4950.642 kb on - strand, within AO356_22115 at 4950.750 kb on - strand, within AO356_22115 at 4950.797 kb on + strand, within AO356_22115 at 4950.797 kb on + strand, within AO356_22115 at 4950.797 kb on + strand, within AO356_22115 at 4950.797 kb on + strand, within AO356_22115 at 4950.797 kb on + strand, within AO356_22115 at 4950.797 kb on + strand, within AO356_22115 at 4950.798 kb on - strand, within AO356_22115 at 4950.798 kb on - strand, within AO356_22115 at 4950.798 kb on - strand, within AO356_22115 at 4950.798 kb on - strand, within AO356_22115 at 4950.824 kb on + strand, within AO356_22115 at 4950.825 kb on - strand, within AO356_22115 at 4950.825 kb on - strand, within AO356_22115 at 4950.825 kb on - strand, within AO356_22115 at 4950.825 kb on - strand, within AO356_22115 at 4950.830 kb on + strand, within AO356_22115 at 4950.830 kb on + strand, within AO356_22115 at 4950.830 kb on + strand, within AO356_22115 at 4950.830 kb on + strand, within AO356_22115 at 4950.830 kb on + strand, within AO356_22115 at 4950.831 kb on - strand, within AO356_22115 at 4950.838 kb on + strand, within AO356_22115 at 4950.839 kb on - strand, within AO356_22115 at 4951.050 kb on - strand at 4951.050 kb on - strand at 4951.119 kb on + strand at 4951.119 kb on + strand at 4951.119 kb on + strand at 4951.120 kb on - strand at 4951.120 kb on - strand at 4951.120 kb on - strand at 4951.161 kb on + strand at 4951.161 kb on + strand at 4951.161 kb on + strand at 4951.161 kb on + strand at 4951.161 kb on + strand at 4951.161 kb on + strand at 4951.162 kb on - strand at 4951.162 kb on - strand at 4951.162 kb on - strand at 4951.162 kb on - strand at 4951.162 kb on - strand at 4951.217 kb on - strand at 4951.230 kb on + strand at 4951.259 kb on - strand at 4951.259 kb on - strand at 4951.259 kb on - strand at 4951.292 kb on + strand at 4951.293 kb on - strand at 4951.301 kb on + strand at 4951.301 kb on + strand at 4951.302 kb on - strand at 4951.302 kb on - strand at 4951.323 kb on - strand, within AO356_22120 at 4951.432 kb on + strand, within AO356_22120 at 4951.433 kb on - strand, within AO356_22120 at 4951.433 kb on - strand, within AO356_22120 at 4951.509 kb on + strand, within AO356_22120 at 4951.509 kb on + strand, within AO356_22120 at 4951.509 kb on + strand, within AO356_22120 at 4951.529 kb on - strand, within AO356_22120
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 5 mM remove 4,948,664 - -0.5 4,948,664 - -1.3 4,948,707 + -0.6 4,948,707 + -0.3 4,948,707 + +0.3 4,948,713 + +0.1 4,948,714 - +0.7 4,948,721 + +0.9 4,948,722 - -0.0 4,948,722 - +0.0 4,948,722 - +1.2 4,948,732 - -0.3 4,948,732 - -1.0 4,948,732 - +0.2 4,948,734 + -0.4 4,948,735 - -0.9 4,948,735 - -0.5 4,948,735 - +0.3 4,948,735 - +0.4 4,948,735 - -0.2 4,948,735 - -0.3 4,948,789 + -0.4 4,948,789 + -0.7 4,948,789 + +0.3 4,948,790 - +0.2 4,949,156 - AO356_22105 0.59 -0.1 4,950,641 + AO356_22115 0.11 +0.2 4,950,641 + AO356_22115 0.11 +0.0 4,950,642 - AO356_22115 0.11 +1.6 4,950,642 - AO356_22115 0.11 +0.5 4,950,750 - AO356_22115 0.35 -0.4 4,950,797 + AO356_22115 0.46 +0.7 4,950,797 + AO356_22115 0.46 -0.0 4,950,797 + AO356_22115 0.46 -1.1 4,950,797 + AO356_22115 0.46 -0.4 4,950,797 + AO356_22115 0.46 +1.0 4,950,797 + AO356_22115 0.46 +0.5 4,950,798 - AO356_22115 0.46 -0.1 4,950,798 - AO356_22115 0.46 +1.6 4,950,798 - AO356_22115 0.46 -0.1 4,950,798 - AO356_22115 0.46 -1.0 4,950,824 + AO356_22115 0.52 -0.1 4,950,825 - AO356_22115 0.52 -0.1 4,950,825 - AO356_22115 0.52 -0.1 4,950,825 - AO356_22115 0.52 +0.7 4,950,825 - AO356_22115 0.52 -0.4 4,950,830 + AO356_22115 0.53 -0.5 4,950,830 + AO356_22115 0.53 -0.9 4,950,830 + AO356_22115 0.53 -0.3 4,950,830 + AO356_22115 0.53 -0.1 4,950,830 + AO356_22115 0.53 -0.4 4,950,831 - AO356_22115 0.53 +0.5 4,950,838 + AO356_22115 0.55 +0.6 4,950,839 - AO356_22115 0.55 +0.3 4,951,050 - +0.4 4,951,050 - +0.5 4,951,119 + -0.6 4,951,119 + +1.6 4,951,119 + +0.3 4,951,120 - -0.3 4,951,120 - +2.5 4,951,120 - +0.5 4,951,161 + +0.4 4,951,161 + -0.5 4,951,161 + -0.4 4,951,161 + -0.7 4,951,161 + -0.2 4,951,161 + -0.2 4,951,162 - -0.2 4,951,162 - +0.3 4,951,162 - -0.5 4,951,162 - +0.7 4,951,162 - +0.3 4,951,217 - -0.7 4,951,230 + -0.1 4,951,259 - -1.3 4,951,259 - +0.5 4,951,259 - +1.6 4,951,292 + -0.3 4,951,293 - -0.6 4,951,301 + -1.6 4,951,301 + -0.1 4,951,302 - +0.1 4,951,302 - +0.9 4,951,323 - AO356_22120 0.12 -0.9 4,951,432 + AO356_22120 0.25 +0.6 4,951,433 - AO356_22120 0.25 -0.8 4,951,433 - AO356_22120 0.25 -0.6 4,951,509 + AO356_22120 0.33 +0.9 4,951,509 + AO356_22120 0.33 +0.7 4,951,509 + AO356_22120 0.33 +0.1 4,951,529 - AO356_22120 0.36 +0.8
Or see this region's nucleotide sequence