Experiment: 1-ethyl-3-methylimidazolium acetate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_02225 and AO356_02230 are separated by 63 nucleotides AO356_02230 and AO356_02235 overlap by 8 nucleotides AO356_02235 and AO356_02240 are separated by 94 nucleotides AO356_02240 and AO356_02245 overlap by 4 nucleotides
AO356_02225: AO356_02225 - multidrug ABC transporter substrate-binding protein, at 477,187 to 478,431
_02225
AO356_02230: AO356_02230 - ABC transporter, at 478,495 to 479,193
_02230
AO356_02235: AO356_02235 - cell division protein FtsX, at 479,186 to 480,436
_02235
AO356_02240: AO356_02240 - pilus assembly protein PilZ, at 480,531 to 481,127
_02240
AO356_02245: AO356_02245 - glycerophosphodiester phosphodiesterase, at 481,124 to 481,846
_02245
Position (kb)
479
480
481 Strain fitness (log2 ratio)
-1
0
1
2 at 480.497 kb on - strand at 480.497 kb on - strand at 480.497 kb on - strand at 480.497 kb on - strand at 480.542 kb on - strand at 480.542 kb on - strand at 480.542 kb on - strand at 480.542 kb on - strand at 480.565 kb on - strand at 480.750 kb on + strand, within AO356_02240 at 480.917 kb on - strand, within AO356_02240 at 481.047 kb on + strand, within AO356_02240 at 481.048 kb on - strand, within AO356_02240 at 481.106 kb on + strand at 481.107 kb on - strand at 481.107 kb on - strand at 481.107 kb on - strand at 481.107 kb on - strand at 481.112 kb on + strand at 481.136 kb on + strand at 481.136 kb on + strand at 481.136 kb on + strand at 481.137 kb on - strand at 481.137 kb on - strand at 481.137 kb on - strand at 481.137 kb on - strand at 481.137 kb on - strand at 481.212 kb on + strand, within AO356_02245 at 481.213 kb on - strand, within AO356_02245 at 481.343 kb on + strand, within AO356_02245 at 481.343 kb on + strand, within AO356_02245 at 481.343 kb on + strand, within AO356_02245 at 481.343 kb on + strand, within AO356_02245 at 481.344 kb on - strand, within AO356_02245 at 481.344 kb on - strand, within AO356_02245
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 5 mM remove 480,497 - +1.8 480,497 - +0.7 480,497 - +0.7 480,497 - -1.2 480,542 - +0.6 480,542 - -1.1 480,542 - +1.0 480,542 - +0.4 480,565 - -0.1 480,750 + AO356_02240 0.37 -0.7 480,917 - AO356_02240 0.65 +0.8 481,047 + AO356_02240 0.86 +0.2 481,048 - AO356_02240 0.87 +0.2 481,106 + -0.1 481,107 - -0.5 481,107 - +0.7 481,107 - +0.5 481,107 - +0.6 481,112 + +0.7 481,136 + -0.3 481,136 + +0.1 481,136 + -0.7 481,137 - +0.7 481,137 - +0.2 481,137 - +0.3 481,137 - +0.1 481,137 - +0.6 481,212 + AO356_02245 0.12 +0.4 481,213 - AO356_02245 0.12 -0.6 481,343 + AO356_02245 0.30 +0.6 481,343 + AO356_02245 0.30 +0.4 481,343 + AO356_02245 0.30 +0.0 481,343 + AO356_02245 0.30 +0.5 481,344 - AO356_02245 0.30 +1.1 481,344 - AO356_02245 0.30 +0.8
Or see this region's nucleotide sequence