Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_01220

Experiment: 1-ethyl-3-methylimidazolium acetate 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_01215 and AO356_01220 overlap by 29 nucleotidesAO356_01220 and AO356_01225 are separated by 192 nucleotides AO356_01215: AO356_01215 - cellulose biosynthesis protein, at 278,652 to 279,830 _01215 AO356_01220: AO356_01220 - hypothetical protein, at 279,802 to 280,593 _01220 AO356_01225: AO356_01225 - alpha/beta hydrolase, at 280,786 to 281,724 _01225 Position (kb) 279 280 281Strain fitness (log2 ratio) -2 -1 0 1 2at 279.174 kb on + strand, within AO356_01215at 279.432 kb on + strand, within AO356_01215at 279.433 kb on - strand, within AO356_01215at 279.465 kb on + strand, within AO356_01215at 279.465 kb on + strand, within AO356_01215at 279.466 kb on - strand, within AO356_01215at 279.466 kb on - strand, within AO356_01215at 279.466 kb on - strand, within AO356_01215at 279.471 kb on + strand, within AO356_01215at 279.471 kb on + strand, within AO356_01215at 279.471 kb on + strand, within AO356_01215at 279.472 kb on - strand, within AO356_01215at 279.472 kb on - strand, within AO356_01215at 279.597 kb on - strand, within AO356_01215at 279.660 kb on + strand, within AO356_01215at 279.720 kb on - strandat 279.777 kb on - strandat 279.945 kb on - strand, within AO356_01220at 280.202 kb on + strand, within AO356_01220at 280.203 kb on - strand, within AO356_01220at 280.203 kb on - strand, within AO356_01220at 280.203 kb on - strand, within AO356_01220at 280.203 kb on - strand, within AO356_01220at 280.206 kb on + strand, within AO356_01220at 280.206 kb on + strand, within AO356_01220at 280.207 kb on - strand, within AO356_01220at 280.207 kb on - strand, within AO356_01220at 280.207 kb on - strand, within AO356_01220at 280.207 kb on - strand, within AO356_01220at 280.207 kb on - strand, within AO356_01220at 280.207 kb on - strand, within AO356_01220at 280.207 kb on - strand, within AO356_01220at 280.207 kb on - strand, within AO356_01220at 280.207 kb on - strand, within AO356_01220at 280.207 kb on - strand, within AO356_01220at 280.299 kb on + strand, within AO356_01220at 280.300 kb on - strand, within AO356_01220at 280.301 kb on + strand, within AO356_01220at 280.301 kb on + strand, within AO356_01220at 280.302 kb on - strand, within AO356_01220at 280.302 kb on - strand, within AO356_01220at 280.302 kb on - strand, within AO356_01220at 280.326 kb on - strand, within AO356_01220at 280.370 kb on - strand, within AO356_01220at 280.430 kb on + strand, within AO356_01220at 280.430 kb on + strand, within AO356_01220at 280.431 kb on - strand, within AO356_01220at 280.431 kb on - strand, within AO356_01220at 280.474 kb on + strand, within AO356_01220at 280.474 kb on + strand, within AO356_01220at 280.474 kb on + strand, within AO356_01220at 280.474 kb on + strand, within AO356_01220at 280.475 kb on - strand, within AO356_01220at 280.475 kb on - strand, within AO356_01220at 280.475 kb on - strand, within AO356_01220at 280.749 kb on + strandat 280.799 kb on + strandat 280.800 kb on - strandat 280.891 kb on - strand, within AO356_01225at 281.059 kb on + strand, within AO356_01225at 281.060 kb on - strand, within AO356_01225at 281.060 kb on - strand, within AO356_01225at 281.201 kb on - strand, within AO356_01225at 281.201 kb on - strand, within AO356_01225at 281.269 kb on + strand, within AO356_01225at 281.269 kb on + strand, within AO356_01225at 281.269 kb on + strand, within AO356_01225at 281.269 kb on + strand, within AO356_01225at 281.270 kb on - strand, within AO356_01225at 281.418 kb on + strand, within AO356_01225at 281.418 kb on + strand, within AO356_01225at 281.419 kb on - strand, within AO356_01225at 281.419 kb on - strand, within AO356_01225at 281.419 kb on - strand, within AO356_01225at 281.419 kb on - strand, within AO356_01225at 281.524 kb on + strand, within AO356_01225

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 5 mM
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279,174 + AO356_01215 0.44 -1.4
279,432 + AO356_01215 0.66 -0.2
279,433 - AO356_01215 0.66 -0.9
279,465 + AO356_01215 0.69 +0.7
279,465 + AO356_01215 0.69 +1.0
279,466 - AO356_01215 0.69 -0.3
279,466 - AO356_01215 0.69 -1.8
279,466 - AO356_01215 0.69 +0.2
279,471 + AO356_01215 0.69 +0.5
279,471 + AO356_01215 0.69 +0.8
279,471 + AO356_01215 0.69 +0.5
279,472 - AO356_01215 0.70 -1.9
279,472 - AO356_01215 0.70 -1.5
279,597 - AO356_01215 0.80 +0.6
279,660 + AO356_01215 0.85 -0.0
279,720 - +0.5
279,777 - -0.2
279,945 - AO356_01220 0.18 -0.3
280,202 + AO356_01220 0.51 -0.3
280,203 - AO356_01220 0.51 -0.3
280,203 - AO356_01220 0.51 +0.4
280,203 - AO356_01220 0.51 +0.1
280,203 - AO356_01220 0.51 +1.2
280,206 + AO356_01220 0.51 -0.3
280,206 + AO356_01220 0.51 +0.7
280,207 - AO356_01220 0.51 +1.9
280,207 - AO356_01220 0.51 +0.3
280,207 - AO356_01220 0.51 +0.1
280,207 - AO356_01220 0.51 +0.6
280,207 - AO356_01220 0.51 +0.3
280,207 - AO356_01220 0.51 -0.7
280,207 - AO356_01220 0.51 -0.5
280,207 - AO356_01220 0.51 +1.5
280,207 - AO356_01220 0.51 -0.3
280,207 - AO356_01220 0.51 -0.6
280,299 + AO356_01220 0.63 -0.1
280,300 - AO356_01220 0.63 +0.0
280,301 + AO356_01220 0.63 -0.9
280,301 + AO356_01220 0.63 -0.5
280,302 - AO356_01220 0.63 +0.1
280,302 - AO356_01220 0.63 +0.6
280,302 - AO356_01220 0.63 -0.4
280,326 - AO356_01220 0.66 +0.3
280,370 - AO356_01220 0.72 +0.7
280,430 + AO356_01220 0.79 +0.5
280,430 + AO356_01220 0.79 +1.2
280,431 - AO356_01220 0.79 +0.6
280,431 - AO356_01220 0.79 +0.9
280,474 + AO356_01220 0.85 +0.5
280,474 + AO356_01220 0.85 +0.6
280,474 + AO356_01220 0.85 +1.2
280,474 + AO356_01220 0.85 -0.2
280,475 - AO356_01220 0.85 -0.6
280,475 - AO356_01220 0.85 -0.4
280,475 - AO356_01220 0.85 +0.5
280,749 + +0.5
280,799 + -0.8
280,800 - +0.1
280,891 - AO356_01225 0.11 -1.2
281,059 + AO356_01225 0.29 -0.5
281,060 - AO356_01225 0.29 -0.6
281,060 - AO356_01225 0.29 -0.1
281,201 - AO356_01225 0.44 +1.1
281,201 - AO356_01225 0.44 +1.7
281,269 + AO356_01225 0.51 -0.4
281,269 + AO356_01225 0.51 -1.2
281,269 + AO356_01225 0.51 +0.2
281,269 + AO356_01225 0.51 +0.5
281,270 - AO356_01225 0.52 +1.2
281,418 + AO356_01225 0.67 +0.1
281,418 + AO356_01225 0.67 -0.1
281,419 - AO356_01225 0.67 -0.2
281,419 - AO356_01225 0.67 +0.1
281,419 - AO356_01225 0.67 +0.8
281,419 - AO356_01225 0.67 -0.1
281,524 + AO356_01225 0.79 -0.1

Or see this region's nucleotide sequence