Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gpsA and Shew_3804 are separated by 6 nucleotides Shew_3804 and Shew_3805 are separated by 207 nucleotides Shew_3805 and Shew_3806 are separated by 163 nucleotides
Shew_3803: gpsA - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase (RefSeq), at 4,526,514 to 4,527,533
gpsA
Shew_3804: Shew_3804 - preprotein translocase subunit SecB (RefSeq), at 4,527,540 to 4,528,028
_3804
Shew_3805: Shew_3805 - rhodanese domain-containing protein (RefSeq), at 4,528,236 to 4,528,748
_3805
Shew_3806: Shew_3806 - phosphoglyceromutase (RefSeq), at 4,528,912 to 4,530,456
_3806
Position (kb) 4528
4529 Strain fitness (log2 ratio) -1
0
1 at 4528.196 kb on - strand at 4528.307 kb on - strand, within Shew_3805 at 4528.307 kb on - strand, within Shew_3805 at 4528.361 kb on - strand, within Shew_3805 at 4528.371 kb on - strand, within Shew_3805 at 4528.489 kb on - strand, within Shew_3805 at 4528.611 kb on - strand, within Shew_3805 at 4528.715 kb on + strand at 4528.807 kb on + strand at 4528.810 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 4,528,196 - -0.1 4,528,307 - Shew_3805 0.14 -0.7 4,528,307 - Shew_3805 0.14 -0.5 4,528,361 - Shew_3805 0.24 -0.7 4,528,371 - Shew_3805 0.26 +0.0 4,528,489 - Shew_3805 0.49 -0.4 4,528,611 - Shew_3805 0.73 -0.2 4,528,715 + +0.0 4,528,807 + -0.3 4,528,810 - +0.2
Or see this region's nucleotide sequence