Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2431 and Shew_2432 overlap by 7 nucleotides Shew_2432 and Shew_2433 are separated by 84 nucleotides Shew_2433 and Shew_2434 overlap by 8 nucleotides
Shew_2431: Shew_2431 - TPR repeat-containing protein (RefSeq), at 2,856,557 to 2,858,638
_2431
Shew_2432: Shew_2432 - hypothetical protein (RefSeq), at 2,858,632 to 2,860,272
_2432
Shew_2433: Shew_2433 - RNA polymerase sigma factor (RefSeq), at 2,860,357 to 2,860,920
_2433
Shew_2434: Shew_2434 - hypothetical protein (RefSeq), at 2,860,913 to 2,861,617
_2434
Position (kb)
2858
2859
2860
2861 Strain fitness (log2 ratio)
-1
0
1 at 2857.826 kb on - strand, within Shew_2431 at 2857.958 kb on + strand, within Shew_2431 at 2859.083 kb on - strand, within Shew_2432 at 2859.233 kb on + strand, within Shew_2432 at 2859.233 kb on + strand, within Shew_2432 at 2859.350 kb on + strand, within Shew_2432 at 2859.360 kb on + strand, within Shew_2432 at 2859.368 kb on - strand, within Shew_2432 at 2859.688 kb on - strand, within Shew_2432 at 2859.876 kb on - strand, within Shew_2432 at 2860.113 kb on - strand at 2860.120 kb on - strand at 2861.144 kb on + strand, within Shew_2434
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 2,857,826 - Shew_2431 0.61 -0.2 2,857,958 + Shew_2431 0.67 -0.2 2,859,083 - Shew_2432 0.27 -0.5 2,859,233 + Shew_2432 0.37 -0.4 2,859,233 + Shew_2432 0.37 -1.7 2,859,350 + Shew_2432 0.44 -0.2 2,859,360 + Shew_2432 0.44 +0.6 2,859,368 - Shew_2432 0.45 -1.1 2,859,688 - Shew_2432 0.64 +0.3 2,859,876 - Shew_2432 0.76 -0.3 2,860,113 - -0.3 2,860,120 - -0.2 2,861,144 + Shew_2434 0.33 -0.5
Or see this region's nucleotide sequence