Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1858 and Shew_1859 are separated by 254 nucleotides Shew_1859 and Shew_1860 are separated by 139 nucleotides Shew_1860 and Shew_1861 are separated by 6 nucleotides
Shew_1858: Shew_1858 - DegT/DnrJ/EryC1/StrS aminotransferase (RefSeq), at 2,137,104 to 2,138,291
_1858
Shew_1859: Shew_1859 - carbonate dehydratase (RefSeq), at 2,138,546 to 2,139,157
_1859
Shew_1860: Shew_1860 - alpha/beta hydrolase fold (RefSeq), at 2,139,297 to 2,140,130
_1860
Shew_1861: Shew_1861 - peptidase M15B and M15C, D,D-carboxypeptidase VanY/endolysin (RefSeq), at 2,140,137 to 2,140,889
_1861
Position (kb)
2138
2139
2140 Strain fitness (log2 ratio)
-1
0
1 at 2138.930 kb on + strand, within Shew_1859 at 2139.227 kb on + strand at 2139.448 kb on + strand, within Shew_1860 at 2139.450 kb on - strand, within Shew_1860 at 2139.712 kb on + strand, within Shew_1860 at 2139.886 kb on - strand, within Shew_1860 at 2139.902 kb on - strand, within Shew_1860 at 2140.028 kb on - strand, within Shew_1860
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 2,138,930 + Shew_1859 0.63 -0.5 2,139,227 + +0.1 2,139,448 + Shew_1860 0.18 -0.1 2,139,450 - Shew_1860 0.18 -0.2 2,139,712 + Shew_1860 0.50 +0.3 2,139,886 - Shew_1860 0.71 -0.1 2,139,902 - Shew_1860 0.73 +0.4 2,140,028 - Shew_1860 0.88 -0.0
Or see this region's nucleotide sequence