Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1058 and Shew_1059 are separated by 266 nucleotides Shew_1059 and Shew_1060 are separated by 66 nucleotides Shew_1060 and Shew_1061 are separated by 146 nucleotides
Shew_1058: Shew_1058 - holo-acyl-carrier-protein synthase (RefSeq), at 1,220,313 to 1,220,714
_1058
Shew_1059: Shew_1059 - hypothetical protein (RefSeq), at 1,220,981 to 1,221,523
_1059
Shew_1060: Shew_1060 - hypothetical protein (RefSeq), at 1,221,590 to 1,222,873
_1060
Shew_1061: Shew_1061 - cytochrome c assembly protein (RefSeq), at 1,223,020 to 1,223,808
_1061
Position (kb)
1221
1222
1223 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1220.644 kb on + strand, within Shew_1058 at 1221.033 kb on - strand at 1221.293 kb on - strand, within Shew_1059 at 1221.431 kb on - strand, within Shew_1059 at 1221.491 kb on + strand at 1221.602 kb on + strand at 1221.635 kb on - strand at 1221.673 kb on + strand at 1221.713 kb on - strand at 1222.110 kb on + strand, within Shew_1060 at 1222.168 kb on + strand, within Shew_1060 at 1222.481 kb on - strand, within Shew_1060 at 1222.553 kb on + strand, within Shew_1060 at 1222.986 kb on - strand at 1223.012 kb on + strand at 1223.093 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 1,220,644 + Shew_1058 0.82 +0.8 1,221,033 - +0.2 1,221,293 - Shew_1059 0.57 +0.1 1,221,431 - Shew_1059 0.83 +0.3 1,221,491 + +0.1 1,221,602 + -0.1 1,221,635 - +0.0 1,221,673 + -0.4 1,221,713 - -0.8 1,222,110 + Shew_1060 0.40 -0.0 1,222,168 + Shew_1060 0.45 -0.6 1,222,481 - Shew_1060 0.69 -0.7 1,222,553 + Shew_1060 0.75 -0.3 1,222,986 - -0.1 1,223,012 + -0.4 1,223,093 + -3.0
Or see this region's nucleotide sequence