Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1811 and Dshi_1812 are separated by 64 nucleotides Dshi_1812 and Dshi_1813 are separated by 73 nucleotides Dshi_1813 and Dshi_R0034 are separated by 122 nucleotides Dshi_R0034 and Dshi_R0035 are separated by 106 nucleotides Dshi_R0035 and Dshi_1814 are separated by 341 nucleotides
Dshi_1811: Dshi_1811 - dihydrolipoamide dehydrogenase (RefSeq), at 1,887,561 to 1,888,958
_1811
Dshi_1812: Dshi_1812 - protein of unknown function DUF924 (RefSeq), at 1,889,023 to 1,889,616
_1812
Dshi_1813: Dshi_1813 - major facilitator superfamily MFS_1 (RefSeq), at 1,889,690 to 1,890,979
_1813
Dshi_R0034: Dshi_R0034 - tRNA-Asp (RefSeq), at 1,891,102 to 1,891,178
_R0034
Dshi_R0035: Dshi_R0035 - tRNA-Asp (RefSeq), at 1,891,285 to 1,891,361
_R0035
Dshi_1814: Dshi_1814 - hypothetical protein (RefSeq), at 1,891,703 to 1,892,863
_1814
Position (kb)
1889
1890
1891 Strain fitness (log2 ratio)
-2
-1
0
1 at 1888.916 kb on + strand at 1888.921 kb on + strand at 1889.047 kb on + strand at 1889.111 kb on - strand, within Dshi_1812 at 1889.119 kb on - strand, within Dshi_1812 at 1889.438 kb on - strand, within Dshi_1812 at 1889.540 kb on - strand, within Dshi_1812 at 1889.600 kb on + strand at 1889.622 kb on - strand at 1889.892 kb on - strand, within Dshi_1813 at 1889.925 kb on + strand, within Dshi_1813 at 1890.063 kb on + strand, within Dshi_1813 at 1890.278 kb on - strand, within Dshi_1813 at 1890.321 kb on + strand, within Dshi_1813 at 1890.531 kb on + strand, within Dshi_1813 at 1890.539 kb on - strand, within Dshi_1813 at 1890.560 kb on + strand, within Dshi_1813 at 1890.573 kb on - strand, within Dshi_1813 at 1890.591 kb on - strand, within Dshi_1813 at 1890.607 kb on - strand, within Dshi_1813 at 1890.642 kb on + strand, within Dshi_1813 at 1890.650 kb on - strand, within Dshi_1813 at 1890.650 kb on - strand, within Dshi_1813 at 1890.801 kb on + strand, within Dshi_1813 at 1890.869 kb on + strand at 1890.869 kb on + strand at 1890.959 kb on - strand at 1890.960 kb on - strand at 1890.960 kb on - strand at 1890.982 kb on - strand at 1891.246 kb on + strand at 1891.251 kb on - strand at 1891.483 kb on - strand at 1891.594 kb on + strand at 1891.594 kb on + strand at 1891.604 kb on + strand at 1891.607 kb on - strand at 1891.628 kb on + strand at 1891.628 kb on + strand at 1891.636 kb on - strand at 1891.636 kb on - strand at 1891.684 kb on + strand at 1891.685 kb on - strand at 1891.692 kb on + strand at 1891.692 kb on - strand at 1891.734 kb on + strand at 1891.740 kb on - strand at 1891.825 kb on + strand, within Dshi_1814 at 1891.831 kb on - strand, within Dshi_1814 at 1891.869 kb on - strand, within Dshi_1814 at 1891.960 kb on - strand, within Dshi_1814
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 1,888,916 + +0.6 1,888,921 + +1.5 1,889,047 + -0.4 1,889,111 - Dshi_1812 0.15 +0.5 1,889,119 - Dshi_1812 0.16 +0.7 1,889,438 - Dshi_1812 0.70 +0.7 1,889,540 - Dshi_1812 0.87 +1.4 1,889,600 + -0.2 1,889,622 - -0.5 1,889,892 - Dshi_1813 0.16 -1.6 1,889,925 + Dshi_1813 0.18 -0.5 1,890,063 + Dshi_1813 0.29 -0.4 1,890,278 - Dshi_1813 0.46 -0.5 1,890,321 + Dshi_1813 0.49 -0.4 1,890,531 + Dshi_1813 0.65 -1.3 1,890,539 - Dshi_1813 0.66 -0.9 1,890,560 + Dshi_1813 0.67 +0.2 1,890,573 - Dshi_1813 0.68 +0.1 1,890,591 - Dshi_1813 0.70 -0.4 1,890,607 - Dshi_1813 0.71 -1.4 1,890,642 + Dshi_1813 0.74 +0.2 1,890,650 - Dshi_1813 0.74 +0.0 1,890,650 - Dshi_1813 0.74 +0.2 1,890,801 + Dshi_1813 0.86 -1.9 1,890,869 + -0.1 1,890,869 + +0.3 1,890,959 - -0.5 1,890,960 - -0.0 1,890,960 - -0.4 1,890,982 - +0.4 1,891,246 + -0.5 1,891,251 - +0.4 1,891,483 - -0.5 1,891,594 + -0.7 1,891,594 + +1.0 1,891,604 + -0.3 1,891,607 - -1.4 1,891,628 + -0.7 1,891,628 + +0.4 1,891,636 - +0.5 1,891,636 - -0.6 1,891,684 + -0.3 1,891,685 - -0.8 1,891,692 + -0.5 1,891,692 - +0.0 1,891,734 + -1.1 1,891,740 - -1.3 1,891,825 + Dshi_1814 0.11 -0.6 1,891,831 - Dshi_1814 0.11 -0.3 1,891,869 - Dshi_1814 0.14 +1.2 1,891,960 - Dshi_1814 0.22 +0.5
Or see this region's nucleotide sequence