Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1305 and Dshi_1306 are separated by 1 nucleotides Dshi_1306 and Dshi_1307 are separated by 341 nucleotides Dshi_1307 and Dshi_1308 overlap by 10 nucleotides Dshi_1308 and Dshi_1309 are separated by 63 nucleotides
Dshi_1305: Dshi_1305 - 17 kDa surface antigen (RefSeq), at 1,359,760 to 1,359,978
_1305
Dshi_1306: Dshi_1306 - hypothetical protein (RefSeq), at 1,359,980 to 1,360,588
_1306
Dshi_1307: Dshi_1307 - NADH-ubiquinone/plastoquinone oxidoreductase chain 3 (RefSeq), at 1,360,930 to 1,361,295
_1307
Dshi_1308: Dshi_1308 - NADH-quinone oxidoreductase, B subunit (RefSeq), at 1,361,286 to 1,361,819
_1308
Dshi_1309: Dshi_1309 - NADH (or F420H2) dehydrogenase, subunit C (RefSeq), at 1,361,883 to 1,362,530
_1309
Position (kb)
1360
1361
1362 Strain fitness (log2 ratio)
-1
0
1 at 1360.249 kb on + strand, within Dshi_1306 at 1360.257 kb on - strand, within Dshi_1306 at 1360.262 kb on + strand, within Dshi_1306 at 1360.289 kb on + strand, within Dshi_1306 at 1360.308 kb on - strand, within Dshi_1306 at 1360.332 kb on - strand, within Dshi_1306 at 1360.489 kb on + strand, within Dshi_1306 at 1360.559 kb on - strand at 1360.562 kb on - strand at 1360.634 kb on - strand at 1360.653 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 1,360,249 + Dshi_1306 0.44 +0.5 1,360,257 - Dshi_1306 0.45 -0.1 1,360,262 + Dshi_1306 0.46 -0.8 1,360,289 + Dshi_1306 0.51 -1.7 1,360,308 - Dshi_1306 0.54 +0.3 1,360,332 - Dshi_1306 0.58 -0.1 1,360,489 + Dshi_1306 0.84 -1.7 1,360,559 - -0.3 1,360,562 - -0.0 1,360,634 - -0.9 1,360,653 - +0.1
Or see this region's nucleotide sequence