Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0711 and Dshi_0712 are separated by 90 nucleotides Dshi_0712 and Dshi_0713 are separated by 105 nucleotides Dshi_0713 and Dshi_0714 overlap by 20 nucleotides
Dshi_0710: Dshi_0710 - hypothetical protein (RefSeq), at 718,730 to 719,377
_0710
Dshi_0711: Dshi_0711 - transcriptional regulator, LysR family (RefSeq), at 719,323 to 720,249
_0711
Dshi_0712: Dshi_0712 - Lysine exporter protein (LYSE/YGGA) (RefSeq), at 720,340 to 720,966
_0712
Dshi_0713: Dshi_0713 - Poly(3-hydroxybutyrate) depolymerase-like protein (RefSeq), at 721,072 to 721,935
_0713
Dshi_0714: Dshi_0714 - protein of unknown function DUF6 transmembrane (RefSeq), at 721,916 to 722,884
_0714
Position (kb) 720
721 Strain fitness (log2 ratio) -1
0
1 at 719.396 kb on + strand at 719.396 kb on + strand at 719.422 kb on + strand, within Dshi_0711 at 719.638 kb on - strand, within Dshi_0711 at 719.681 kb on - strand, within Dshi_0711 at 719.787 kb on - strand, within Dshi_0711 at 719.787 kb on - strand, within Dshi_0711 at 719.880 kb on - strand, within Dshi_0711 at 719.936 kb on - strand, within Dshi_0711 at 719.938 kb on + strand, within Dshi_0711 at 719.982 kb on + strand, within Dshi_0711 at 720.083 kb on + strand, within Dshi_0711 at 720.091 kb on - strand, within Dshi_0711 at 720.134 kb on + strand, within Dshi_0711 at 720.168 kb on + strand at 720.330 kb on + strand at 720.330 kb on + strand at 720.338 kb on - strand at 720.353 kb on + strand at 720.355 kb on - strand at 720.368 kb on + strand at 720.455 kb on + strand, within Dshi_0712 at 720.474 kb on + strand, within Dshi_0712 at 720.608 kb on + strand, within Dshi_0712 at 720.648 kb on - strand, within Dshi_0712 at 720.680 kb on + strand, within Dshi_0712 at 720.685 kb on + strand, within Dshi_0712 at 720.705 kb on + strand, within Dshi_0712 at 720.840 kb on - strand, within Dshi_0712 at 720.865 kb on + strand, within Dshi_0712 at 720.892 kb on + strand, within Dshi_0712 at 720.906 kb on - strand at 720.964 kb on - strand at 720.995 kb on + strand at 720.995 kb on + strand at 721.101 kb on - strand at 721.178 kb on + strand, within Dshi_0713 at 721.265 kb on - strand, within Dshi_0713 at 721.453 kb on + strand, within Dshi_0713 at 721.470 kb on + strand, within Dshi_0713 at 721.473 kb on + strand, within Dshi_0713 at 721.539 kb on + strand, within Dshi_0713 at 721.710 kb on + strand, within Dshi_0713 at 721.715 kb on + strand, within Dshi_0713 at 721.795 kb on - strand, within Dshi_0713 at 721.831 kb on - strand, within Dshi_0713 at 721.889 kb on + strand at 721.904 kb on + strand at 721.908 kb on + strand at 721.923 kb on + strand at 721.943 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 719,396 + -0.3 719,396 + +0.8 719,422 + Dshi_0711 0.11 -0.4 719,638 - Dshi_0711 0.34 -0.0 719,681 - Dshi_0711 0.39 -0.6 719,787 - Dshi_0711 0.50 +0.5 719,787 - Dshi_0711 0.50 +0.5 719,880 - Dshi_0711 0.60 -0.1 719,936 - Dshi_0711 0.66 +0.4 719,938 + Dshi_0711 0.66 -1.2 719,982 + Dshi_0711 0.71 +0.0 720,083 + Dshi_0711 0.82 +0.0 720,091 - Dshi_0711 0.83 +0.1 720,134 + Dshi_0711 0.87 -0.1 720,168 + +0.1 720,330 + +0.4 720,330 + +1.1 720,338 - -0.1 720,353 + +1.2 720,355 - +1.1 720,368 + -0.1 720,455 + Dshi_0712 0.18 +0.1 720,474 + Dshi_0712 0.21 +0.5 720,608 + Dshi_0712 0.43 +1.1 720,648 - Dshi_0712 0.49 -0.4 720,680 + Dshi_0712 0.54 -0.6 720,685 + Dshi_0712 0.55 -1.7 720,705 + Dshi_0712 0.58 -0.5 720,840 - Dshi_0712 0.80 -1.0 720,865 + Dshi_0712 0.84 -0.8 720,892 + Dshi_0712 0.88 +0.1 720,906 - -1.2 720,964 - -0.0 720,995 + -0.5 720,995 + -0.6 721,101 - +1.1 721,178 + Dshi_0713 0.12 -0.0 721,265 - Dshi_0713 0.22 +0.6 721,453 + Dshi_0713 0.44 +1.1 721,470 + Dshi_0713 0.46 +1.3 721,473 + Dshi_0713 0.46 -0.0 721,539 + Dshi_0713 0.54 +0.8 721,710 + Dshi_0713 0.74 -0.1 721,715 + Dshi_0713 0.74 -0.1 721,795 - Dshi_0713 0.84 -0.2 721,831 - Dshi_0713 0.88 +0.4 721,889 + -0.0 721,904 + -0.2 721,908 + -1.3 721,923 + -0.1 721,943 + -0.5
Or see this region's nucleotide sequence