Experiment: antimonate 0.005 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2602 and Pf6N2E2_2603 are separated by 249 nucleotides Pf6N2E2_2603 and Pf6N2E2_2604 are separated by 83 nucleotides Pf6N2E2_2604 and Pf6N2E2_2605 are separated by 83 nucleotides Pf6N2E2_2605 and Pf6N2E2_2606 overlap by 18 nucleotides
Pf6N2E2_2602 - Flagellin protein FlaA, at 3,059,480 to 3,060,331
_2602
Pf6N2E2_2603 - hypothetical protein, at 3,060,581 to 3,060,763
_2603
Pf6N2E2_2604 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 3,060,847 to 3,062,139
_2604
Pf6N2E2_2605 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180), at 3,062,223 to 3,063,104
_2605
Pf6N2E2_2606 - hypothetical protein, at 3,063,087 to 3,063,332
_2606
Position (kb)
3060
3061
3062
3063 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3059.899 kb on + strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3060.059 kb on - strand, within Pf6N2E2_2602 at 3060.059 kb on - strand, within Pf6N2E2_2602 at 3060.059 kb on - strand, within Pf6N2E2_2602 at 3060.118 kb on + strand, within Pf6N2E2_2602 at 3060.118 kb on + strand, within Pf6N2E2_2602 at 3060.119 kb on - strand, within Pf6N2E2_2602 at 3060.119 kb on - strand, within Pf6N2E2_2602 at 3060.119 kb on - strand, within Pf6N2E2_2602 at 3060.172 kb on + strand, within Pf6N2E2_2602 at 3060.173 kb on - strand, within Pf6N2E2_2602 at 3060.317 kb on + strand at 3060.323 kb on + strand at 3060.393 kb on + strand at 3060.394 kb on - strand at 3060.407 kb on - strand at 3060.409 kb on + strand at 3060.409 kb on + strand at 3060.409 kb on + strand at 3060.410 kb on - strand at 3060.410 kb on - strand at 3060.410 kb on - strand at 3060.413 kb on + strand at 3060.414 kb on - strand at 3060.414 kb on - strand at 3060.437 kb on + strand at 3060.437 kb on + strand at 3060.505 kb on - strand at 3060.622 kb on - strand, within Pf6N2E2_2603 at 3060.622 kb on - strand, within Pf6N2E2_2603 at 3060.622 kb on - strand, within Pf6N2E2_2603 at 3060.631 kb on - strand, within Pf6N2E2_2603 at 3060.671 kb on + strand, within Pf6N2E2_2603 at 3060.671 kb on + strand, within Pf6N2E2_2603 at 3060.672 kb on - strand, within Pf6N2E2_2603 at 3060.672 kb on - strand, within Pf6N2E2_2603 at 3060.672 kb on - strand, within Pf6N2E2_2603 at 3060.672 kb on - strand, within Pf6N2E2_2603 at 3060.672 kb on - strand, within Pf6N2E2_2603 at 3060.686 kb on + strand, within Pf6N2E2_2603 at 3060.686 kb on + strand, within Pf6N2E2_2603 at 3060.687 kb on - strand, within Pf6N2E2_2603 at 3060.687 kb on - strand, within Pf6N2E2_2603 at 3060.687 kb on - strand, within Pf6N2E2_2603 at 3060.687 kb on - strand, within Pf6N2E2_2603 at 3060.687 kb on - strand, within Pf6N2E2_2603 at 3060.858 kb on - strand at 3060.858 kb on - strand at 3060.962 kb on + strand at 3060.962 kb on + strand at 3060.963 kb on - strand at 3061.174 kb on + strand, within Pf6N2E2_2604 at 3061.174 kb on + strand, within Pf6N2E2_2604 at 3061.175 kb on - strand, within Pf6N2E2_2604 at 3061.175 kb on - strand, within Pf6N2E2_2604 at 3061.217 kb on + strand, within Pf6N2E2_2604 at 3061.217 kb on + strand, within Pf6N2E2_2604 at 3061.218 kb on - strand, within Pf6N2E2_2604 at 3061.218 kb on - strand, within Pf6N2E2_2604 at 3061.218 kb on - strand, within Pf6N2E2_2604 at 3061.296 kb on - strand, within Pf6N2E2_2604 at 3061.347 kb on + strand, within Pf6N2E2_2604 at 3061.348 kb on - strand, within Pf6N2E2_2604 at 3061.348 kb on - strand, within Pf6N2E2_2604 at 3061.348 kb on - strand, within Pf6N2E2_2604 at 3061.348 kb on - strand, within Pf6N2E2_2604 at 3061.414 kb on - strand, within Pf6N2E2_2604 at 3061.498 kb on - strand, within Pf6N2E2_2604 at 3061.682 kb on + strand, within Pf6N2E2_2604 at 3061.682 kb on + strand, within Pf6N2E2_2604 at 3061.683 kb on - strand, within Pf6N2E2_2604 at 3061.754 kb on + strand, within Pf6N2E2_2604 at 3061.754 kb on + strand, within Pf6N2E2_2604 at 3061.754 kb on + strand, within Pf6N2E2_2604 at 3061.911 kb on - strand, within Pf6N2E2_2604 at 3061.911 kb on - strand, within Pf6N2E2_2604 at 3061.911 kb on - strand, within Pf6N2E2_2604 at 3061.998 kb on + strand, within Pf6N2E2_2604 at 3061.999 kb on - strand, within Pf6N2E2_2604 at 3062.107 kb on + strand at 3062.108 kb on - strand at 3062.170 kb on + strand at 3062.183 kb on + strand at 3062.263 kb on - strand at 3062.337 kb on - strand, within Pf6N2E2_2605 at 3062.337 kb on - strand, within Pf6N2E2_2605 at 3062.785 kb on + strand, within Pf6N2E2_2605 at 3062.786 kb on - strand, within Pf6N2E2_2605 at 3062.786 kb on - strand, within Pf6N2E2_2605 at 3062.795 kb on - strand, within Pf6N2E2_2605 at 3062.795 kb on - strand, within Pf6N2E2_2605 at 3062.800 kb on + strand, within Pf6N2E2_2605 at 3062.801 kb on - strand, within Pf6N2E2_2605 at 3062.822 kb on + strand, within Pf6N2E2_2605 at 3062.823 kb on - strand, within Pf6N2E2_2605 at 3062.823 kb on - strand, within Pf6N2E2_2605 at 3062.823 kb on - strand, within Pf6N2E2_2605 at 3062.844 kb on - strand, within Pf6N2E2_2605 at 3063.094 kb on + strand at 3063.095 kb on - strand at 3063.095 kb on - strand at 3063.095 kb on - strand at 3063.095 kb on - strand at 3063.095 kb on - strand at 3063.122 kb on - strand, within Pf6N2E2_2606 at 3063.122 kb on - strand, within Pf6N2E2_2606 at 3063.138 kb on + strand, within Pf6N2E2_2606 at 3063.139 kb on - strand, within Pf6N2E2_2606
Per-strain Table
Position Strand Gene LocusTag Fraction antimonate 0.005 M remove 3,059,899 + Pf6N2E2_2602 0.49 -1.2 3,059,900 - Pf6N2E2_2602 0.49 +0.3 3,059,900 - Pf6N2E2_2602 0.49 +0.2 3,059,900 - Pf6N2E2_2602 0.49 +1.3 3,059,900 - Pf6N2E2_2602 0.49 -0.7 3,059,900 - Pf6N2E2_2602 0.49 +0.1 3,059,900 - Pf6N2E2_2602 0.49 +0.1 3,060,059 - Pf6N2E2_2602 0.68 -2.4 3,060,059 - Pf6N2E2_2602 0.68 -0.5 3,060,059 - Pf6N2E2_2602 0.68 +0.5 3,060,118 + Pf6N2E2_2602 0.75 +0.6 3,060,118 + Pf6N2E2_2602 0.75 +0.2 3,060,119 - Pf6N2E2_2602 0.75 -1.1 3,060,119 - Pf6N2E2_2602 0.75 +0.2 3,060,119 - Pf6N2E2_2602 0.75 -0.5 3,060,172 + Pf6N2E2_2602 0.81 +1.2 3,060,173 - Pf6N2E2_2602 0.81 +0.7 3,060,317 + -0.0 3,060,323 + +0.8 3,060,393 + +0.9 3,060,394 - +0.4 3,060,407 - +0.1 3,060,409 + +0.0 3,060,409 + -0.2 3,060,409 + +0.7 3,060,410 - +1.0 3,060,410 - -0.8 3,060,410 - -0.1 3,060,413 + +0.8 3,060,414 - +0.8 3,060,414 - -0.5 3,060,437 + +0.4 3,060,437 + -0.3 3,060,505 - -0.4 3,060,622 - Pf6N2E2_2603 0.22 +0.6 3,060,622 - Pf6N2E2_2603 0.22 +0.4 3,060,622 - Pf6N2E2_2603 0.22 +0.2 3,060,631 - Pf6N2E2_2603 0.27 -0.6 3,060,671 + Pf6N2E2_2603 0.49 -1.2 3,060,671 + Pf6N2E2_2603 0.49 +1.1 3,060,672 - Pf6N2E2_2603 0.50 +0.2 3,060,672 - Pf6N2E2_2603 0.50 +0.1 3,060,672 - Pf6N2E2_2603 0.50 -0.6 3,060,672 - Pf6N2E2_2603 0.50 -0.5 3,060,672 - Pf6N2E2_2603 0.50 +1.0 3,060,686 + Pf6N2E2_2603 0.57 -1.0 3,060,686 + Pf6N2E2_2603 0.57 +0.2 3,060,687 - Pf6N2E2_2603 0.58 +1.0 3,060,687 - Pf6N2E2_2603 0.58 +0.2 3,060,687 - Pf6N2E2_2603 0.58 -0.0 3,060,687 - Pf6N2E2_2603 0.58 +0.4 3,060,687 - Pf6N2E2_2603 0.58 +0.3 3,060,858 - +1.0 3,060,858 - -0.3 3,060,962 + +0.1 3,060,962 + +1.0 3,060,963 - +0.1 3,061,174 + Pf6N2E2_2604 0.25 -1.3 3,061,174 + Pf6N2E2_2604 0.25 -0.5 3,061,175 - Pf6N2E2_2604 0.25 -0.3 3,061,175 - Pf6N2E2_2604 0.25 -0.4 3,061,217 + Pf6N2E2_2604 0.29 +0.1 3,061,217 + Pf6N2E2_2604 0.29 -1.0 3,061,218 - Pf6N2E2_2604 0.29 +0.0 3,061,218 - Pf6N2E2_2604 0.29 +0.2 3,061,218 - Pf6N2E2_2604 0.29 -0.6 3,061,296 - Pf6N2E2_2604 0.35 -0.1 3,061,347 + Pf6N2E2_2604 0.39 +0.8 3,061,348 - Pf6N2E2_2604 0.39 +0.7 3,061,348 - Pf6N2E2_2604 0.39 -1.4 3,061,348 - Pf6N2E2_2604 0.39 +0.6 3,061,348 - Pf6N2E2_2604 0.39 -0.6 3,061,414 - Pf6N2E2_2604 0.44 +0.1 3,061,498 - Pf6N2E2_2604 0.50 -0.9 3,061,682 + Pf6N2E2_2604 0.65 +1.1 3,061,682 + Pf6N2E2_2604 0.65 -0.8 3,061,683 - Pf6N2E2_2604 0.65 -0.6 3,061,754 + Pf6N2E2_2604 0.70 +0.2 3,061,754 + Pf6N2E2_2604 0.70 -0.3 3,061,754 + Pf6N2E2_2604 0.70 +1.1 3,061,911 - Pf6N2E2_2604 0.82 +0.4 3,061,911 - Pf6N2E2_2604 0.82 +0.5 3,061,911 - Pf6N2E2_2604 0.82 -0.5 3,061,998 + Pf6N2E2_2604 0.89 +0.1 3,061,999 - Pf6N2E2_2604 0.89 +0.5 3,062,107 + -0.8 3,062,108 - +0.6 3,062,170 + +0.0 3,062,183 + +2.1 3,062,263 - +0.4 3,062,337 - Pf6N2E2_2605 0.13 +1.2 3,062,337 - Pf6N2E2_2605 0.13 +1.8 3,062,785 + Pf6N2E2_2605 0.64 +2.0 3,062,786 - Pf6N2E2_2605 0.64 +0.4 3,062,786 - Pf6N2E2_2605 0.64 +1.6 3,062,795 - Pf6N2E2_2605 0.65 -0.3 3,062,795 - Pf6N2E2_2605 0.65 +0.7 3,062,800 + Pf6N2E2_2605 0.65 +1.4 3,062,801 - Pf6N2E2_2605 0.66 +0.4 3,062,822 + Pf6N2E2_2605 0.68 +0.2 3,062,823 - Pf6N2E2_2605 0.68 +1.8 3,062,823 - Pf6N2E2_2605 0.68 +1.1 3,062,823 - Pf6N2E2_2605 0.68 +0.4 3,062,844 - Pf6N2E2_2605 0.70 +2.8 3,063,094 + +1.0 3,063,095 - +2.8 3,063,095 - +1.8 3,063,095 - +1.4 3,063,095 - +1.1 3,063,095 - +0.2 3,063,122 - Pf6N2E2_2606 0.14 +1.3 3,063,122 - Pf6N2E2_2606 0.14 +0.9 3,063,138 + Pf6N2E2_2606 0.21 +1.1 3,063,139 - Pf6N2E2_2606 0.21 +0.1
Or see this region's nucleotide sequence