Experiment: D-Raffinose 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS05310 and LRK54_RS05315 are separated by 377 nucleotides LRK54_RS05315 and LRK54_RS05320 are separated by 174 nucleotides LRK54_RS05320 and LRK54_RS05325 are separated by 91 nucleotides
LRK54_RS05310: LRK54_RS05310 - homoserine O-succinyltransferase, at 1,140,836 to 1,141,858
_RS05310
LRK54_RS05315: LRK54_RS05315 - cysteine synthase A, at 1,142,236 to 1,143,255
_RS05315
LRK54_RS05320: LRK54_RS05320 - 2OG-Fe dioxygenase family protein, at 1,143,430 to 1,144,152
_RS05320
LRK54_RS05325: LRK54_RS05325 - DUF1304 domain-containing protein, at 1,144,244 to 1,144,606
_RS05325
Position (kb)
1142
1143
1144 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1141.410 kb on + strand, within LRK54_RS05310 at 1141.411 kb on - strand, within LRK54_RS05310 at 1141.411 kb on - strand, within LRK54_RS05310 at 1141.701 kb on + strand, within LRK54_RS05310 at 1141.701 kb on + strand, within LRK54_RS05310 at 1141.701 kb on + strand, within LRK54_RS05310 at 1141.701 kb on + strand, within LRK54_RS05310 at 1141.701 kb on + strand, within LRK54_RS05310 at 1141.701 kb on + strand, within LRK54_RS05310 at 1141.701 kb on + strand, within LRK54_RS05310 at 1141.702 kb on - strand, within LRK54_RS05310 at 1141.702 kb on - strand, within LRK54_RS05310 at 1141.702 kb on - strand, within LRK54_RS05310 at 1141.702 kb on - strand, within LRK54_RS05310 at 1141.702 kb on - strand, within LRK54_RS05310 at 1141.702 kb on - strand, within LRK54_RS05310 at 1141.702 kb on - strand, within LRK54_RS05310 at 1141.734 kb on + strand, within LRK54_RS05310 at 1141.734 kb on + strand, within LRK54_RS05310 at 1141.893 kb on - strand at 1142.114 kb on + strand at 1142.120 kb on + strand at 1142.120 kb on + strand at 1142.121 kb on - strand at 1142.121 kb on - strand at 1142.122 kb on + strand at 1142.122 kb on + strand at 1142.122 kb on + strand at 1142.122 kb on + strand at 1142.122 kb on + strand at 1142.122 kb on + strand at 1142.122 kb on + strand at 1142.124 kb on + strand at 1142.124 kb on + strand at 1142.124 kb on + strand at 1142.125 kb on - strand at 1142.125 kb on - strand at 1142.125 kb on - strand at 1142.125 kb on - strand at 1142.125 kb on - strand at 1142.131 kb on - strand at 1142.614 kb on + strand, within LRK54_RS05315 at 1142.614 kb on + strand, within LRK54_RS05315 at 1142.890 kb on + strand, within LRK54_RS05315 at 1142.890 kb on + strand, within LRK54_RS05315 at 1142.890 kb on + strand, within LRK54_RS05315 at 1142.890 kb on + strand, within LRK54_RS05315 at 1142.890 kb on + strand, within LRK54_RS05315 at 1142.890 kb on + strand, within LRK54_RS05315 at 1142.890 kb on + strand, within LRK54_RS05315 at 1142.890 kb on + strand, within LRK54_RS05315 at 1142.890 kb on + strand, within LRK54_RS05315 at 1142.890 kb on + strand, within LRK54_RS05315 at 1142.891 kb on - strand, within LRK54_RS05315 at 1142.891 kb on - strand, within LRK54_RS05315 at 1142.891 kb on - strand, within LRK54_RS05315 at 1142.891 kb on - strand, within LRK54_RS05315 at 1142.891 kb on - strand, within LRK54_RS05315 at 1143.253 kb on + strand at 1143.253 kb on + strand at 1143.254 kb on - strand at 1143.254 kb on - strand at 1143.286 kb on + strand at 1143.286 kb on + strand at 1143.286 kb on + strand at 1143.286 kb on + strand at 1143.286 kb on + strand at 1143.286 kb on + strand at 1143.286 kb on + strand at 1143.332 kb on + strand at 1143.332 kb on + strand at 1143.332 kb on + strand at 1143.332 kb on + strand at 1143.332 kb on + strand at 1143.332 kb on + strand at 1143.332 kb on + strand at 1143.333 kb on - strand at 1143.418 kb on + strand at 1143.589 kb on + strand, within LRK54_RS05320 at 1143.589 kb on + strand, within LRK54_RS05320 at 1143.589 kb on + strand, within LRK54_RS05320 at 1143.589 kb on + strand, within LRK54_RS05320 at 1143.589 kb on + strand, within LRK54_RS05320 at 1143.589 kb on + strand, within LRK54_RS05320 at 1143.589 kb on + strand, within LRK54_RS05320 at 1143.589 kb on + strand, within LRK54_RS05320 at 1143.589 kb on + strand, within LRK54_RS05320 at 1143.589 kb on + strand, within LRK54_RS05320 at 1143.589 kb on + strand, within LRK54_RS05320 at 1143.589 kb on + strand, within LRK54_RS05320 at 1143.589 kb on + strand, within LRK54_RS05320 at 1143.589 kb on + strand, within LRK54_RS05320 at 1143.590 kb on - strand, within LRK54_RS05320 at 1143.590 kb on - strand, within LRK54_RS05320 at 1143.590 kb on - strand, within LRK54_RS05320 at 1143.590 kb on - strand, within LRK54_RS05320 at 1143.590 kb on - strand, within LRK54_RS05320 at 1143.590 kb on - strand, within LRK54_RS05320 at 1143.590 kb on - strand, within LRK54_RS05320 at 1143.590 kb on - strand, within LRK54_RS05320 at 1143.590 kb on - strand, within LRK54_RS05320 at 1143.590 kb on - strand, within LRK54_RS05320 at 1143.590 kb on - strand, within LRK54_RS05320 at 1143.590 kb on - strand, within LRK54_RS05320 at 1143.590 kb on - strand, within LRK54_RS05320 at 1143.590 kb on - strand, within LRK54_RS05320 at 1143.590 kb on - strand, within LRK54_RS05320 at 1143.590 kb on - strand, within LRK54_RS05320 at 1143.685 kb on + strand, within LRK54_RS05320 at 1143.685 kb on + strand, within LRK54_RS05320 at 1143.685 kb on + strand, within LRK54_RS05320 at 1143.685 kb on + strand, within LRK54_RS05320 at 1143.685 kb on + strand, within LRK54_RS05320 at 1143.685 kb on + strand, within LRK54_RS05320 at 1143.686 kb on - strand, within LRK54_RS05320 at 1143.686 kb on - strand, within LRK54_RS05320 at 1143.700 kb on + strand, within LRK54_RS05320 at 1143.700 kb on + strand, within LRK54_RS05320 at 1143.700 kb on + strand, within LRK54_RS05320 at 1143.700 kb on + strand, within LRK54_RS05320 at 1143.700 kb on + strand, within LRK54_RS05320 at 1143.700 kb on + strand, within LRK54_RS05320 at 1143.700 kb on + strand, within LRK54_RS05320 at 1143.700 kb on + strand, within LRK54_RS05320 at 1143.700 kb on + strand, within LRK54_RS05320 at 1143.701 kb on - strand, within LRK54_RS05320 at 1143.701 kb on - strand, within LRK54_RS05320 at 1143.701 kb on - strand, within LRK54_RS05320 at 1143.701 kb on - strand, within LRK54_RS05320 at 1143.701 kb on - strand, within LRK54_RS05320 at 1143.701 kb on - strand, within LRK54_RS05320 at 1143.928 kb on + strand, within LRK54_RS05320 at 1143.928 kb on + strand, within LRK54_RS05320 at 1143.929 kb on - strand, within LRK54_RS05320 at 1143.929 kb on - strand, within LRK54_RS05320 at 1144.130 kb on + strand at 1144.130 kb on + strand at 1144.130 kb on + strand at 1144.130 kb on + strand at 1144.131 kb on - strand at 1144.131 kb on - strand at 1144.131 kb on - strand at 1144.131 kb on - strand at 1144.184 kb on + strand at 1144.184 kb on + strand at 1144.184 kb on + strand at 1144.184 kb on + strand at 1144.184 kb on + strand at 1144.185 kb on - strand at 1144.185 kb on - strand at 1144.185 kb on - strand at 1144.185 kb on - strand at 1144.185 kb on - strand at 1144.208 kb on + strand at 1144.209 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Raffinose 20 mM remove 1,141,410 + LRK54_RS05310 0.56 -1.7 1,141,411 - LRK54_RS05310 0.56 -1.1 1,141,411 - LRK54_RS05310 0.56 -1.4 1,141,701 + LRK54_RS05310 0.85 -0.0 1,141,701 + LRK54_RS05310 0.85 -2.0 1,141,701 + LRK54_RS05310 0.85 -1.0 1,141,701 + LRK54_RS05310 0.85 -1.0 1,141,701 + LRK54_RS05310 0.85 -1.3 1,141,701 + LRK54_RS05310 0.85 +0.6 1,141,701 + LRK54_RS05310 0.85 -1.7 1,141,702 - LRK54_RS05310 0.85 -1.3 1,141,702 - LRK54_RS05310 0.85 -0.4 1,141,702 - LRK54_RS05310 0.85 -1.3 1,141,702 - LRK54_RS05310 0.85 -0.6 1,141,702 - LRK54_RS05310 0.85 -1.5 1,141,702 - LRK54_RS05310 0.85 -2.1 1,141,702 - LRK54_RS05310 0.85 -1.8 1,141,734 + LRK54_RS05310 0.88 -1.1 1,141,734 + LRK54_RS05310 0.88 -2.8 1,141,893 - -0.5 1,142,114 + +1.5 1,142,120 + -0.3 1,142,120 + -0.7 1,142,121 - +0.1 1,142,121 - -0.5 1,142,122 + -1.1 1,142,122 + -0.2 1,142,122 + -0.3 1,142,122 + -1.0 1,142,122 + -0.6 1,142,122 + +1.1 1,142,122 + -0.1 1,142,124 + +1.0 1,142,124 + +0.8 1,142,124 + +1.2 1,142,125 - -1.2 1,142,125 - +0.6 1,142,125 - -0.7 1,142,125 - +0.8 1,142,125 - -0.4 1,142,131 - -0.1 1,142,614 + LRK54_RS05315 0.37 -0.5 1,142,614 + LRK54_RS05315 0.37 +0.6 1,142,890 + LRK54_RS05315 0.64 +0.2 1,142,890 + LRK54_RS05315 0.64 -0.0 1,142,890 + LRK54_RS05315 0.64 +0.0 1,142,890 + LRK54_RS05315 0.64 +0.8 1,142,890 + LRK54_RS05315 0.64 +0.8 1,142,890 + LRK54_RS05315 0.64 -0.0 1,142,890 + LRK54_RS05315 0.64 +0.7 1,142,890 + LRK54_RS05315 0.64 -1.5 1,142,890 + LRK54_RS05315 0.64 +1.8 1,142,890 + LRK54_RS05315 0.64 -0.4 1,142,891 - LRK54_RS05315 0.64 -1.4 1,142,891 - LRK54_RS05315 0.64 +1.0 1,142,891 - LRK54_RS05315 0.64 +1.0 1,142,891 - LRK54_RS05315 0.64 +0.5 1,142,891 - LRK54_RS05315 0.64 +1.0 1,143,253 + -1.0 1,143,253 + +0.5 1,143,254 - -0.5 1,143,254 - +0.9 1,143,286 + +0.5 1,143,286 + +0.2 1,143,286 + -0.3 1,143,286 + +1.0 1,143,286 + -0.1 1,143,286 + +0.2 1,143,286 + -0.8 1,143,332 + -0.2 1,143,332 + -0.9 1,143,332 + -0.1 1,143,332 + -1.0 1,143,332 + -0.3 1,143,332 + -0.8 1,143,332 + +0.1 1,143,333 - +0.3 1,143,418 + -0.3 1,143,589 + LRK54_RS05320 0.22 +2.2 1,143,589 + LRK54_RS05320 0.22 -0.4 1,143,589 + LRK54_RS05320 0.22 -0.5 1,143,589 + LRK54_RS05320 0.22 +0.6 1,143,589 + LRK54_RS05320 0.22 +0.5 1,143,589 + LRK54_RS05320 0.22 -1.4 1,143,589 + LRK54_RS05320 0.22 -0.1 1,143,589 + LRK54_RS05320 0.22 -0.1 1,143,589 + LRK54_RS05320 0.22 -0.2 1,143,589 + LRK54_RS05320 0.22 -0.1 1,143,589 + LRK54_RS05320 0.22 -0.4 1,143,589 + LRK54_RS05320 0.22 -0.7 1,143,589 + LRK54_RS05320 0.22 +0.3 1,143,589 + LRK54_RS05320 0.22 +0.4 1,143,590 - LRK54_RS05320 0.22 -0.7 1,143,590 - LRK54_RS05320 0.22 -0.1 1,143,590 - LRK54_RS05320 0.22 -0.5 1,143,590 - LRK54_RS05320 0.22 -0.4 1,143,590 - LRK54_RS05320 0.22 -0.5 1,143,590 - LRK54_RS05320 0.22 +0.4 1,143,590 - LRK54_RS05320 0.22 -0.5 1,143,590 - LRK54_RS05320 0.22 +0.9 1,143,590 - LRK54_RS05320 0.22 +1.8 1,143,590 - LRK54_RS05320 0.22 -0.2 1,143,590 - LRK54_RS05320 0.22 -1.2 1,143,590 - LRK54_RS05320 0.22 -0.5 1,143,590 - LRK54_RS05320 0.22 +1.2 1,143,590 - LRK54_RS05320 0.22 -1.0 1,143,590 - LRK54_RS05320 0.22 +1.0 1,143,590 - LRK54_RS05320 0.22 -0.5 1,143,685 + LRK54_RS05320 0.35 +0.2 1,143,685 + LRK54_RS05320 0.35 +0.6 1,143,685 + LRK54_RS05320 0.35 +0.2 1,143,685 + LRK54_RS05320 0.35 +0.7 1,143,685 + LRK54_RS05320 0.35 +0.2 1,143,685 + LRK54_RS05320 0.35 -0.8 1,143,686 - LRK54_RS05320 0.35 -0.3 1,143,686 - LRK54_RS05320 0.35 +0.6 1,143,700 + LRK54_RS05320 0.37 +0.1 1,143,700 + LRK54_RS05320 0.37 -0.2 1,143,700 + LRK54_RS05320 0.37 +0.2 1,143,700 + LRK54_RS05320 0.37 +1.8 1,143,700 + LRK54_RS05320 0.37 -0.8 1,143,700 + LRK54_RS05320 0.37 -1.5 1,143,700 + LRK54_RS05320 0.37 -0.2 1,143,700 + LRK54_RS05320 0.37 +0.8 1,143,700 + LRK54_RS05320 0.37 +1.4 1,143,701 - LRK54_RS05320 0.37 +0.2 1,143,701 - LRK54_RS05320 0.37 +2.1 1,143,701 - LRK54_RS05320 0.37 -0.6 1,143,701 - LRK54_RS05320 0.37 -1.9 1,143,701 - LRK54_RS05320 0.37 +1.5 1,143,701 - LRK54_RS05320 0.37 +1.0 1,143,928 + LRK54_RS05320 0.69 -0.4 1,143,928 + LRK54_RS05320 0.69 -0.1 1,143,929 - LRK54_RS05320 0.69 -0.3 1,143,929 - LRK54_RS05320 0.69 +0.8 1,144,130 + -0.6 1,144,130 + -0.8 1,144,130 + +0.1 1,144,130 + -0.2 1,144,131 - -0.6 1,144,131 - +1.6 1,144,131 - -0.3 1,144,131 - +0.8 1,144,184 + -0.1 1,144,184 + +0.2 1,144,184 + -1.2 1,144,184 + -0.3 1,144,184 + -0.6 1,144,185 - +0.1 1,144,185 - +0.5 1,144,185 - -0.2 1,144,185 - +1.3 1,144,185 - +0.4 1,144,208 + -2.4 1,144,209 - +1.0
Or see this region's nucleotide sequence