Experiment: Fusidic 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2764 and Shew_2765 are separated by 182 nucleotides Shew_2765 and Shew_2766 are separated by 371 nucleotides Shew_2766 and motB are separated by 254 nucleotides
Shew_2764: Shew_2764 - DNA-binding transcriptional activator GcvA (RefSeq), at 3,269,102 to 3,270,013
_2764
Shew_2765: Shew_2765 - hypothetical protein (RefSeq), at 3,270,196 to 3,270,426
_2765
Shew_2766: Shew_2766 - thiamine biosynthesis protein ThiI (RefSeq), at 3,270,798 to 3,272,255
_2766
Shew_2767: motB - flagellar motor protein MotB (RefSeq), at 3,272,510 to 3,273,454
motB
Position (kb)
3270
3271
3272
3273 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3270.528 kb on + strand at 3270.528 kb on + strand at 3270.528 kb on + strand at 3270.536 kb on - strand at 3270.987 kb on + strand, within Shew_2766 at 3271.109 kb on + strand, within Shew_2766 at 3271.134 kb on + strand, within Shew_2766 at 3271.797 kb on - strand, within Shew_2766 at 3272.128 kb on + strand at 3272.530 kb on + strand at 3272.799 kb on + strand, within motB at 3272.799 kb on + strand, within motB at 3272.799 kb on + strand, within motB at 3272.807 kb on - strand, within motB at 3272.846 kb on - strand, within motB
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.03125 mg/ml remove 3,270,528 + +0.5 3,270,528 + -0.0 3,270,528 + -0.4 3,270,536 - -0.1 3,270,987 + Shew_2766 0.13 -0.1 3,271,109 + Shew_2766 0.21 -0.3 3,271,134 + Shew_2766 0.23 -0.3 3,271,797 - Shew_2766 0.69 -0.8 3,272,128 + -3.2 3,272,530 + -0.1 3,272,799 + motB Shew_2767 0.31 -0.2 3,272,799 + motB Shew_2767 0.31 +0.1 3,272,799 + motB Shew_2767 0.31 -0.1 3,272,807 - motB Shew_2767 0.31 +0.9 3,272,846 - motB Shew_2767 0.36 +0.8
Or see this region's nucleotide sequence