Experiment: Fusidic 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2155 and Shew_2156 are separated by 126 nucleotides Shew_2156 and Shew_2157 are separated by 40 nucleotides Shew_2157 and Shew_2158 are separated by 118 nucleotides
Shew_2155: Shew_2155 - 4-hydroxyphenylpyruvate dioxygenase (RefSeq), at 2,499,904 to 2,500,944
_2155
Shew_2156: Shew_2156 - lysine exporter protein LysE/YggA (RefSeq), at 2,501,071 to 2,501,688
_2156
Shew_2157: Shew_2157 - hypothetical protein (RefSeq), at 2,501,729 to 2,503,096
_2157
Shew_2158: Shew_2158 - gamma-glutamyltransferase (RefSeq), at 2,503,215 to 2,504,945
_2158
Position (kb)
2501
2502
2503
2504 Strain fitness (log2 ratio)
-1
0
1 at 2501.139 kb on - strand, within Shew_2156 at 2501.177 kb on - strand, within Shew_2156 at 2501.450 kb on - strand, within Shew_2156 at 2502.218 kb on + strand, within Shew_2157 at 2502.525 kb on - strand, within Shew_2157 at 2502.904 kb on - strand, within Shew_2157 at 2502.983 kb on + strand at 2503.117 kb on - strand at 2503.265 kb on + strand at 2503.351 kb on - strand at 2503.463 kb on + strand, within Shew_2158 at 2503.595 kb on + strand, within Shew_2158 at 2503.919 kb on - strand, within Shew_2158
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.03125 mg/ml remove 2,501,139 - Shew_2156 0.11 -0.1 2,501,177 - Shew_2156 0.17 -0.4 2,501,450 - Shew_2156 0.61 +0.4 2,502,218 + Shew_2157 0.36 -0.3 2,502,525 - Shew_2157 0.58 +0.1 2,502,904 - Shew_2157 0.86 -1.1 2,502,983 + +0.3 2,503,117 - +0.1 2,503,265 + +0.1 2,503,351 - -0.8 2,503,463 + Shew_2158 0.14 -0.1 2,503,595 + Shew_2158 0.22 -0.1 2,503,919 - Shew_2158 0.41 +0.2
Or see this region's nucleotide sequence