Experiment: Fusidic 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1427 and Shew_1428 are separated by 28 nucleotides Shew_1428 and Shew_1429 are separated by 74 nucleotides Shew_1429 and Shew_1430 are separated by 12 nucleotides
Shew_1427: Shew_1427 - 3'(2'),5'-bisphosphate nucleotidase (RefSeq), at 1,643,151 to 1,643,945
_1427
Shew_1428: Shew_1428 - fructokinase (RefSeq), at 1,643,974 to 1,644,864
_1428
Shew_1429: Shew_1429 - hypothetical protein (RefSeq), at 1,644,939 to 1,645,679
_1429
Shew_1430: Shew_1430 - hypothetical protein (RefSeq), at 1,645,692 to 1,646,360
_1430
Position (kb) 1643
1644
1645 Strain fitness (log2 ratio) -1
0
1
2 at 1643.006 kb on - strand at 1643.031 kb on - strand at 1643.292 kb on + strand, within Shew_1427 at 1643.691 kb on - strand, within Shew_1427 at 1643.714 kb on - strand, within Shew_1427 at 1643.875 kb on + strand at 1644.477 kb on + strand, within Shew_1428 at 1644.920 kb on - strand at 1645.179 kb on + strand, within Shew_1429 at 1645.602 kb on - strand, within Shew_1429 at 1645.744 kb on + strand at 1645.744 kb on + strand at 1645.752 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.03125 mg/ml remove 1,643,006 - -1.0 1,643,031 - +0.3 1,643,292 + Shew_1427 0.18 -0.7 1,643,691 - Shew_1427 0.68 -0.9 1,643,714 - Shew_1427 0.71 -0.0 1,643,875 + -0.6 1,644,477 + Shew_1428 0.56 -0.6 1,644,920 - +0.1 1,645,179 + Shew_1429 0.32 -0.1 1,645,602 - Shew_1429 0.89 -0.2 1,645,744 + +0.4 1,645,744 + +0.1 1,645,752 - +2.4
Or see this region's nucleotide sequence