Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3311 and Shew_3312 are separated by 30 nucleotides Shew_3312 and Shew_3313 are separated by 2 nucleotides Shew_3313 and Shew_3314 overlap by 4 nucleotides Shew_3314 and Shew_3315 overlap by 8 nucleotides Shew_3315 and Shew_3316 are separated by 100 nucleotides
Shew_3311: Shew_3311 - RNA polymerase factor sigma-54 (RefSeq), at 3,951,593 to 3,953,080
_3311
Shew_3312: Shew_3312 - sigma 54 modulation protein/ribosomal protein S30EA (RefSeq), at 3,953,111 to 3,953,398
_3312
Shew_3313: Shew_3313 - PTS IIA-like nitrogen-regulatory protein PtsN (RefSeq), at 3,953,401 to 3,953,844
_3313
Shew_3314: Shew_3314 - hypothetical protein (RefSeq), at 3,953,841 to 3,954,689
_3314
Shew_3315: Shew_3315 - phosphotransferase system, phosphocarrier protein HPr (RefSeq), at 3,954,682 to 3,954,957
_3315
Shew_3316: Shew_3316 - magnesium transporter (RefSeq), at 3,955,058 to 3,956,425
_3316
Position (kb)
3953
3954
3955 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6
7 at 3953.051 kb on - strand at 3953.217 kb on - strand, within Shew_3312 at 3953.569 kb on + strand, within Shew_3313 at 3953.569 kb on + strand, within Shew_3313 at 3954.150 kb on - strand, within Shew_3314 at 3954.661 kb on - strand at 3954.696 kb on - strand at 3954.761 kb on - strand, within Shew_3315 at 3955.078 kb on - strand at 3955.217 kb on + strand, within Shew_3316 at 3955.371 kb on - strand, within Shew_3316 at 3955.406 kb on + strand, within Shew_3316
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 3,953,051 - +7.0 3,953,217 - Shew_3312 0.37 +2.4 3,953,569 + Shew_3313 0.38 +2.5 3,953,569 + Shew_3313 0.38 +2.1 3,954,150 - Shew_3314 0.36 +0.7 3,954,661 - -0.4 3,954,696 - -0.8 3,954,761 - Shew_3315 0.29 +1.4 3,955,078 - +2.7 3,955,217 + Shew_3316 0.12 +2.9 3,955,371 - Shew_3316 0.23 +2.9 3,955,406 + Shew_3316 0.25 +2.2
Or see this region's nucleotide sequence